Publication number: 20230203577
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Application
Filed:
August 24, 2022
Publication date:
June 29, 2023
Inventors:
Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas
Publication number: 20220106641
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Application
Filed:
September 13, 2021
Publication date:
April 7, 2022
Inventors:
Phillip BELGRADER, Zachary BENT, Rajiv BHARADWAJ, Vijay Kumar Sreenivasa GOPALAN, Josephine HARADA, Christopher HINDSON, Mohammad Rahimi LENJI, Michael Ybarra LUCERO, Geoffrey McDERMOTT, Elliott MEER, Tarjei Sigurd MIKKELSEN, Christopher Joachim O'KEEFFE, Katherine PFEIFFER, Andrew D. PRICE, Paul RYVKIN, Serge SAXONOV, John R. STUELPNAGEL, Jessica Michele TERRY, Tobias Daniel WHEELER, Indira WU, Solongo Batjargal ZIRALDO, Stephane Claude BOUTET, Sarah TAYLOR, Niranjan SRINIVAS
Publication number: 20210130892
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Application
Filed:
October 29, 2020
Publication date:
May 6, 2021
Inventors:
Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas
Publication number: 20200140944
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Application
Filed:
November 22, 2019
Publication date:
May 7, 2020
Inventors:
Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohamad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo
Publication number: 20200002763
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Application
Filed:
June 12, 2019
Publication date:
January 2, 2020
Inventors:
Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas