Abstract: A solid type nano-pore sequencer is arrayed easily. In a liquid column separation device including first and second base materials, a liquid feeding unit supplies fluids to a gap between the two base materials. Hydrophilic and hydrophobic regions are disposed on a surface of the second base material, and the hydrophilic region is disposed to oppose a predetermined position of the first base material where a substance to be measured is made to pass through. Also, a flow passage space includes at least one inlet and outlet through which fluids can flow in and out. A representative length of the hydrophilic region is made larger than a distance between the first and second base materials, and thereby a liquid column capable of connecting the first base material and the second base material is formed by making two or more immiscible fluids to flow by the liquid feed unit.

Abstract: Provided is a method for generating a trained model for classifying blocking event data representing nanopore blocking events in a biomolecule measurement device. The method includes generating a first trained model by executing machine learning of a training model using first teacher data, the first teacher data includes teacher blocking event data and a teacher label, the teacher label indicates whether the teacher blocking event data is classified as Good data or bad data, and the first trained model is configured to classify the blocking event data into good data or bad data. In addition, a method for determining a base sequence a biomolecule and a biomolecule measurement device are provided.

Abstract: A biomolecule is more easily and reliably reciprocated in a nanopore. An adapter molecule that directly or indirectly binds to a biomolecule to be analyzed comprises a three-dimensional structure formation domain consisting of a single-stranded nucleotide.

Abstract: In a base sequence determination system that performs a complementary strand synthesis reaction of a template DNA strand using DNA polymerase, a complementary strand synthesized by DNA polymerase is degraded by exonuclease in a solution.

Abstract: An apparatus for storing a thin film device, the apparatus including: a thin film device 3 having an insulating thin film containing Si and having a thickness of 100 nm or less; a solution in contact with the thin film; and a container having a tank that seals the solution, wherein the solution is a solution that satisfies any of the following conditions (1) to (3). (1) A solution containing water in a volume ratio of 0% or more to 30% or less (2) A solution cooled and maintained at a temperature equal to or higher than a solidification point and lower than 15° C. (3) A solution that contains a salt with a concentration of 1 mol/L or more and a saturation concentration or less and is cooled and maintained to a temperature equal to or higher than a solidification point and lower than 25° C.

Abstract: An electrophoresis device of the present disclosure includes an electrophoresis path of a sample, a dispersion element for dispersing light from the sample within the electrophoresis path, a photodetector for detecting the light dispersed by the dispersion element, and a computation unit for determining a spectrum of the light on the basis of a signal from the photodetector, and is characterized in that the computation unit corrects the spectrum using correction factors determined for each migration condition or fluorescent dye.

Abstract: An electrophoresis system includes a plurality of capillaries in which electrophoresis of a sample is performed, a light source that irradiates detection positions of the capillaries with light, a light detector that detects light generated by the irradiation with the light by the light source that depends on components contained in the sample, and a buffer storage unit into which one end of the plurality of capillaries are inserted at a time of the electrophoresis of the sample and in which a buffer is stored. A computer controls an electrophoresis condition of each of the plurality of capillaries such that arrival times for the components that move in the plurality of capillaries to reach the detection positions are shifted from each other. As a result, it is possible to detect fluorescence signals at different timings in each of the plurality of capillaries.

Abstract: A double-stranded DNA to be analyzed is analyzed without subjecting it to a modification treatment. An adapter molecule to be bound to the double-stranded DNA to be analyzed has a double-stranded nucleic acid region having base sequences complementary to each other, a pair of single-stranded nucleic acid regions having base sequences non-complementary to each other, and a block molecule placed in one of the single-stranded nucleic acid regions.

Abstract: In an electrophoresis apparatus using a capillary, electrophoresis using a single capillary sometimes requires replacement of a sieving matrix. Replacement with a different sieving matrix has conventionally required cleaning with sieving matrix cleaning liquid, which has increased costs and time required. An electrophoresis apparatus according to the present invention comprises an anodic capillary head provided at a distal end of the capillary, a sieving matrix container filled with a sieving matrix used for electrophoresis, and a filling mechanism for filling the capillary with the sieving matrix via the sieving matrix container. The filling mechanism fills the capillary, which is already filled with the sieving matrix, with a sieving matrix different from the already-filled sieving matrix without using sieving matrix cleaning liquid.

Abstract: An object of the present invention is to provide a biomolecule measuring device that can decrease the influence of crosstalk between chambers. A biomolecule measuring device according to the present invention supplies, to electrodes equipped on chambers, voltages modulated differently to each other.

Abstract: The present invention aims to provide a biological sample analysis device which achieves an improvement in accuracy of an analysis result, a reduction in reagent cost, and shortening of a required time. The biological sample analysis device according to the present invention compares first measurement data acquired by measuring a biological sample and second measurement data acquired by measuring a reference sample and determines that when the difference between the two exceeds a threshold value, it is necessary to remeasure the reference sample before remeasuring the biological sample and reacquire a reference value (refer to FIG. 3A).

Abstract: Provided are a first tank; a second tank; a thin film having a nanopore, which communicates the first tank to the second tank, and disposed between the first and second tanks; a first electrode provided in the first tank; and a second electrode provided in the second tank. A wall surface of the nanopore has an ion adsorption preventing structure to prevent desorption/adsorption of an ion contained in a solution filling the first tank and/or the second tank, and a voltage is applied between the first and second electrodes to measure an ion current flowing through the nanopore.

Abstract: A biological sample analyzer using a nanopore, said analyzer comprising: a first chamber that storing a solvent; a baseboard provided with a nanopore through which a biological sample passes; a second chamber which is positioned adjacently to the first chamber via the baseboard and stores the solvent; a first electrode formed in the first chamber; a second electrode formed in the second chamber; a detector detecting the biological sample which has passed through the nanopore; and a stirrer stirring the solvent in the first chamber.

Abstract: In an analysis method and an analysis system for detecting fluorescences from each of a plurality of light-emitting points in a plurality of wavelength bands in order to identify fluorescence emissions of a plurality of types of fluorophores from the plurality of light-emitting points, spatial crosstalk and spectral crosstalk are present between the plurality of light-emitting points and between the plurality of wavelength bands, and then performance of the identification is reduced. The spatial crosstalk and the spectral crosstalk are eliminated and concentrations of each of the plurality of types of fluorophores at each of the plurality of light-emitting points are derived by inputting all detection signals in the plurality of wavelength bands for the plurality of light-emitting points to a predetermined arithmetic operation expression.

Abstract: A multicapillary electrophoresis device includes: a capillary array formed by arranging a plurality of capillaries; a light source irradiating the capillaries with excitation light; a photodetector detecting fluorescence from a sample in the capillaries; and an arithmetic control unit calculating a signal intensity of the fluorescence according to a signal from the photodetector. The arithmetic control unit is configured so as to correct the signal intensity according to a correction index determined for each of the combinations of any of the capillaries and a fluorophore labeled to the sample.

Abstract: A biopolymer analysis device includes an insulating thin film that is made of an inorganic material, a first liquid tank and a second liquid tank that are separated by the thin film, a plurality of first electrodes that is arranged in the first liquid tank, and a second electrode that is disposed in the second liquid tank. A water-repellent liquid and a plurality of liquid droplets are introduced into the first liquid tank, the plurality of first electrodes is configured to be able to convey the plurality of droplets introduced into the first liquid tank by electro wetting on dielectric by applying a certain voltage, and the plurality of droplets is conveyed to portions coming into contact with the plurality of first electrodes, and is insulated from each other by the water-repellent liquid.

Abstract: The purpose of the present invention is to provide a method for treating biomolecules and a method for analyzing biomolecules with which it is possible to effectively suppress the clog of nanopores. The present invention is a method for treating biomolecules for analysis in which nanopores are used, wherein the method includes a step for preparing a sample solution that includes ammonium cations represented by a prescribed formula and biomolecules in which at least a portion of the higher-order structure has been fused.

Abstract: A genotype analysis device includes an electrophoresis device and a data analysis device. A mobility model management unit including an environment information receiving unit, a prediction model storage unit, and a mobility prediction unit is provided in an STR analysis unit of the data analysis device. The mobility prediction unit generates a prediction model for predicting a correction amount of a standard base length of an allele, based upon an environmental condition at the time of electrophoresis received by the environmental information receiving unit and an electrophoresis result of an allelic ladder. A base length of the allele is corrected from the environmental condition without using the allelic ladder by using the prediction model.

Abstract: Since a few kilovolts of an application voltage is necessary to take in a biological sample, an EWOD electrode, for example, is destroyed, and the electrode becomes non-reusable for moving a droplet. Therefore, an object of the present invention is to provide a biochemical cartridge usable for multiple times for taking in a biological sample by a capillary array, for example, and a biochemical analysis device using the biochemical cartridge. In order to solve the problem, the biochemical cartridge according to the present invention includes a passage through which a sample is transported, a plurality of electrodes disposed on the passage along a direction in which a sample is transported, the plurality of electrodes being provided to transport a sample, and an opening provided opposite to the plurality of electrodes disposed on a downstream side of the passage.

Abstract: Provided is an electrophoresis device for enhancing its analysis performance. In order to achieve the task, the electrophoresis device includes a capillary array constituted by a capillary, a capillary head for bundling an end of the capillary, an electrode holder for holding an electrode provided at the other end of the capillary and a detector provided for the capillary, a first heater for heating the capillary, and an irradiation detection unit for irradiating a light to the detector to detect a fluorescence generated from a fluorescence labelled sample in the capillary. The electrophoresis device further includes a second heater for heating the detector.