Patents by Inventor Mio Morishita
Mio Morishita has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20090029416Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: ApplicationFiled: November 27, 2007Publication date: January 29, 2009Applicant: TAKARA BIO INC.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Publication number: 20080044903Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferring receptor, or introducing a new functional group into the functional substance.Type: ApplicationFiled: July 27, 2007Publication date: February 21, 2008Applicants: Takara Shuzo Co., Ltd., TAKARA BIO INC.Inventors: Mitsuhiro UENO, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Patent number: 7314744Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: GrantFiled: March 16, 2004Date of Patent: January 1, 2008Assignee: Takara Bio Inc.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 7144719Abstract: A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.Type: GrantFiled: July 12, 2004Date of Patent: December 5, 2006Assignee: Takara Bio, Inc.Inventors: Hikaru Takakura, Mio Morishita, Tomoko Shimojo, Kiyozo Asada, Ikunoshi Kato
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Publication number: 20050084934Abstract: A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.Type: ApplicationFiled: July 12, 2004Publication date: April 21, 2005Applicant: Takara Bio, Inc.Inventors: Hikaru Takakura, Mio Morishita, Tomoko Shimojo, Kiyozo Asada, Ikunoshin Kato
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Patent number: 6849441Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID NOs: 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: GrantFiled: April 24, 2001Date of Patent: February 1, 2005Assignee: Takara Shuzo Co., LTDInventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Publication number: 20050014221Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: ApplicationFiled: March 16, 2004Publication date: January 20, 2005Applicant: Takara Shuzo Co., Ltd.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 6787359Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.Type: GrantFiled: January 9, 2001Date of Patent: September 7, 2004Assignee: Takara Shuzo Co., Ltd.Inventors: Mitsuhiro Ueno, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Patent number: 6783970Abstract: A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.Type: GrantFiled: March 6, 2002Date of Patent: August 31, 2004Assignee: Takara Bio Inc.Inventors: Hikaru Takakura, Mio Morishita, Tomoko Shimojo, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20040058447Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.Type: ApplicationFiled: September 9, 2003Publication date: March 25, 2004Applicant: Takara Shuzo Co., Ltd.Inventors: Mitsuhiro Ueno, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Publication number: 20020132335Abstract: A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.Type: ApplicationFiled: March 6, 2002Publication date: September 19, 2002Applicant: Takara Shuzo Co., Ltd.Inventors: Hikaru Takakura, Mio Morishita, Tomoko Shimojo, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20020086402Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID NOs: 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: ApplicationFiled: April 24, 2001Publication date: July 4, 2002Applicant: Takara Shuzo Co., Ltd.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 6358726Abstract: A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.Type: GrantFiled: December 8, 1999Date of Patent: March 19, 2002Assignee: Takara Shuzo Co., Ltd.Inventors: Hikaru Takakura, Mio Morishita, Tomoko Shimojo, Kiyozo Asada, Ikunoshin Kato
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Patent number: 6261822Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: GrantFiled: May 20, 1998Date of Patent: July 17, 2001Assignee: Takara Shuzo Co., Ltd.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 5756339Abstract: There is disclosed a hyperthermostable protease gene originating in Pyrococcus furiosus, in particular, a hyperthermostable protease gene encoding the amino acid sequence represented by the SEQ ID NO 1 in the Sequence Listing or a part thereof which retains the activity of the hyperthermostable protease. There is also disclosed a process for producing the protease by culturing a transformant transformed with a plasmid into which the above gene has been inserted.Type: GrantFiled: December 13, 1996Date of Patent: May 26, 1998Assignee: Takara Shuzo Co., Ltd.Inventors: Masanori Mitta, Katsuhiko Yamamoto, Mio Morishita, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato