Patents by Inventor Monika Glemzaite

Monika Glemzaite has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
    Publication number: 20180187195
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.
    Type: Application
    Filed: December 7, 2017
    Publication date: July 5, 2018
    Applicant: VILNIUS UNIVERSITY
    Inventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS, Arvydas LUBYS, Lolita ZALIAUSKIENE, Monika GLEMZAITE, Anja SMITH
  • RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
    Publication number: 20150050699
    Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.
    Type: Application
    Filed: March 20, 2013
    Publication date: February 19, 2015
    Inventors: Virginijus Siksnys, Giedrius Gasiunas, Tautvydad Karvelis, Arvydas Lubys, Lolita Zaliauskiene, Monika Glemzaite, Anja Smith