Patents by Inventor Nahoko Kobayashi

Nahoko Kobayashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10125168
    Abstract: Provided is a method for adjusting the balance of TNF-R1 and TNF-R2 in cells present at a target organ, tissue or site. In the adjustment method provided, when increasing the relative presence of TNF-R2 compared to the presence of TNF-R1 in the cells, a synthetic peptide is supplied to the cells, with the peptide being essentially made of at a TNF-R1 signal peptide motif or a modified amino acid sequence thereof; and when decreasing the relative presence of TNF-R2 compared to the presence of TNF-R1 in the cells, a synthetic peptide is supplied to the cells, with the peptide being essentially made of a TNF-R2 signal peptide motif or a modified amino acid sequence thereof.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: November 13, 2018
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Patent number: 9480727
    Abstract: This invention provides an artificially synthesized peptide having an activity to inhibit the expression of type 2 TNF receptor (TNF-R2), and a TNF-R2 expression-inhibiting composition including the peptide as an active ingredient. Also provided is a method for inhibiting the expression of TNF-R2 in cells capable of expressing TNF-R2, by using the peptide. The expression of TNF-R2 in the cells is inhibited, by supplying the cells capable of expressing TNF-R2 with a synthetic peptide essentially including of an amino acid sequence constituting a signal peptide in an amyloid precursor protein (APP) or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in/from/to the amino acid sequence of the signal peptide.
    Type: Grant
    Filed: October 17, 2013
    Date of Patent: November 1, 2016
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Patent number: 9370182
    Abstract: A composition including an artificially synthesized antimicrobial peptide that is not present as a mature peptide in nature. The composition includes an artificially synthesized peptide that has an antimicrobial activity against at least one strain of bacteria or fungi and includes an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the amino acid sequence, and further including at least one species of pharmaceutically acceptable carrier.
    Type: Grant
    Filed: May 23, 2013
    Date of Patent: June 21, 2016
    Assignee: Toagosei Co., Ltd.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Patent number: 9365618
    Abstract: This invention provides a TNFR2 expression-inducing composition including as an active ingredient a peptide having TNFR2 expression-inducing activity, and a method for producing cells that express TNFR2 selectively by use of the composition. The cell production method provided by this invention includes: culturing at least one species of cells capable of expressing TNF receptor 2, and supplying the cells with a synthetic peptide consisting of a nuclear localization signal sequence (NLS) or a nucleolar localization signal sequence (NoLS) to enhance TNFR2 expression in the cells.
    Type: Grant
    Filed: April 17, 2012
    Date of Patent: June 14, 2016
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa
  • Patent number: 9353351
    Abstract: This invention provides a multipolar cell including three or more centrosomes dispersed therein and a multipolar spindle formed so as to extend from the three or more respective centrosomes. The multipolar cell production method provided by the present invention supplies cultured eukaryotic cells with a synthetic peptide as a multipolarity-inducing peptide, with the synthetic peptide having an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 10 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence. As the eukaryotic cells, cultured tumor cells are used.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: May 31, 2016
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Yuki Kobayashi
  • Patent number: 9353350
    Abstract: This invention provides a multipolar cell including three or more centrosomes dispersed therein and a multipolar spindle formed so as to extend from the three or more respective centrosomes. The multipolar cell production method provided by the present invention supplies cultured eukaryotic cells with a synthetic peptide as a multipolarity-inducing peptide, with the synthetic peptide having an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 12 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: May 31, 2016
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Yuki Kobayashi
  • Patent number: 9296787
    Abstract: This invention provides an antitumor peptide and an antitumor composition that includes the peptide for suppressing proliferation of at least one species of tumor cells. The antitumor composition provided by this invention includes an antitumor peptide capable of inducing formation of multipolar spindles in at least one species of tumor cells, and at least one species of pharmaceutically acceptable carrier, wherein the antitumor peptide is a synthetic peptide having an amino acid sequence selected from SEQ ID NOs: 1 to 22 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: March 29, 2016
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Patent number: 9238796
    Abstract: The pharmaceutical composition provided by the present invention comprises at least one pharmaceutically acceptable carrier, and an active ingredient including an artificially synthesized peptide comprises: (A) an amino acid sequence constituting a cell-penetrating peptide and (B) an amino acid sequence constituting the signal peptide in amyloid precursor protein (APP) or an N-terminal partial amino acid sequence or C-terminal partial amino acid sequence from the amino acid sequence constituting that signal peptide.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: January 19, 2016
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi
  • Patent number: 9200257
    Abstract: The pharmaceutical composition provided by the present invention comprises at least one pharmaceutically acceptable carrier, and an active ingredient including an artificially synthesized peptide comprises: (A) an amino acid sequence constituting a cell-penetrating peptide and (B) an amino acid sequence constituting the signal peptide in amyloid precursor protein (APP) or an N-terminal partial amino acid sequence or C-terminal partial amino acid sequence from the amino acid sequence constituting that signal peptide.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: December 1, 2015
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi
  • Publication number: 20150273018
    Abstract: This invention provides an artificially synthesized peptide having an activity to inhibit the expression of type 2 TNF receptor (TNF-R2), and a TNF-R2 expression-inhibiting composition including the peptide as an active ingredient. Also provided is a method for inhibiting the expression of TNF-R2 in cells capable of expressing TNF-R2, by using the peptide. The expression of TNF-R2 in the cells is inhibited, by supplying the cells capable of expressing TNF-R2 with a synthetic peptide essentially including of an amino acid sequence constituting a signal peptide in an amyloid precursor protein (APP) or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in/from/to the amino acid sequence of the signal peptide.
    Type: Application
    Filed: October 17, 2013
    Publication date: October 1, 2015
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Patent number: 9133437
    Abstract: A method for manufacturing at least one kind of eukaryotic cells or a biosynthetic substance derived from the eukaryotic cells by proliferating the cells, which includes preparing an artificially synthesized peptide for promoting proliferation of the at least one kind of eukaryotic cells, incubating the eukaryotic cells in a culture medium, and adding the synthesized peptide at least once to the culture medium during the incubation process. The synthesized peptide includes an amino acid sequence selected from SEQ ID NOS: 1 to 18, and an amino acid sequence selected from SEQ ID NOS: 19 to 97.
    Type: Grant
    Filed: January 24, 2014
    Date of Patent: September 15, 2015
    Assignee: TOAGOSEI CO. LTD.
    Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Chikara Shinohara, Masatoki Watanabe, Ken-ichiro Hata
  • Publication number: 20150232512
    Abstract: Provided is a method for adjusting the balance of TNF-R1 and TNF-R2 in cells present at a target organ, tissue or site. In the adjustment method provided, when increasing the relative presence of TNF-R2 compared to the presence of TNF-R1 in the cells, a synthetic peptide is supplied to the cells, with the peptide being essentially made of at a TNF-R1 signal peptide motif or a modified amino acid sequence thereof; and when decreasing the relative presence of TNF-R2 compared to the presence of TNF-R1 in the cells, a synthetic peptide is supplied to the cells, with the peptide being essentially made of a TNF-R2 signal peptide motif or a modified amino acid sequence thereof.
    Type: Application
    Filed: September 20, 2013
    Publication date: August 20, 2015
    Applicant: TOAGOSEI CO., LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Publication number: 20150126434
    Abstract: A composition including an artificially synthesized antimicrobial peptide that is not present as a mature peptide in nature. The composition includes an artificially synthesized peptide that has an antimicrobial activity against at least one strain of bacteria or fungi and includes an amino acid sequence represented by any one of SEQ ID NOs: 1 to 6 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the amino acid sequence, and further including at least one species of pharmaceutically acceptable carrier.
    Type: Application
    Filed: May 23, 2013
    Publication date: May 7, 2015
    Applicant: TOAGOSEI CO., LTD.
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Publication number: 20150018286
    Abstract: This invention provides an antitumor peptide and an antitumor composition that includes the peptide for suppressing proliferation of at least one species of tumor cells. The antitumor composition provided by this invention includes an antitumor peptide capable of inducing formation of multipolar spindles in at least one species of tumor cells, and at least one species of pharmaceutically acceptable carrier, wherein the antitumor peptide is a synthetic peptide having an amino acid sequence selected from SEQ ID NOs: 1 to 22 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence.
    Type: Application
    Filed: December 20, 2012
    Publication date: January 15, 2015
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida
  • Publication number: 20150004697
    Abstract: This invention provides a multipolar cell including three or more centrosomes dispersed therein and a multipolar spindle formed so as to extend from the three or more respective centrosomes. The multipolar cell production method provided by the present invention supplies cultured eukaryotic cells with a synthetic peptide as a multipolarity-inducing peptide, with the synthetic peptide having an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 12 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence.
    Type: Application
    Filed: December 20, 2012
    Publication date: January 1, 2015
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Yuki Kobayashi
  • Publication number: 20140363889
    Abstract: This invention provides a multipolar cell including three or more centrosomes dispersed therein and a multipolar spindle formed so as to extend from the three or more respective centrosomes. The multipolar cell production method provided by the present invention supplies cultured eukaryotic cells with a synthetic peptide as a multipolarity-inducing peptide, with the synthetic peptide having an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 10 or an amino acid sequence formed by substituting, deleting and/or adding one, two or three amino acid residues in/from/to the selected amino acid sequence. As the eukaryotic cells, cultured tumor cells are used.
    Type: Application
    Filed: December 20, 2012
    Publication date: December 11, 2014
    Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Yuki Kobayashi
  • Publication number: 20140335613
    Abstract: The pharmaceutical composition provided by the present invention comprises at least one pharmaceutically acceptable carrier, and an active ingredient including an artificially synthesized peptide comprises: (A) an amino acid sequence constituting a cell-penetrating peptide and (B) an amino acid sequence constituting the signal peptide in amyloid precursor protein (APP) or an N-terminal partial amino acid sequence or C-terminal partial amino acid sequence from the amino acid sequence constituting that signal peptide.
    Type: Application
    Filed: July 25, 2014
    Publication date: November 13, 2014
    Inventors: Tetsuhiko YOSHIDA, Nahoko KOBAYASHI
  • Patent number: 8822408
    Abstract: The pharmaceutical composition includes at least one pharmaceutically acceptable carrier, and an active ingredient including an artificially synthesized peptide includes: (A) an amino acid sequence constituting a cell-penetrating peptide and (B) an amino acid sequence constituting the signal peptide in amyloid precursor protein (APP) or an N-terminal partial amino acid sequence or C-terminal partial amino acid sequence from the amino acid sequence constituting that signal peptide.
    Type: Grant
    Filed: June 3, 2011
    Date of Patent: September 2, 2014
    Assignee: Toagosei Co., Ltd.
    Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi
  • Publication number: 20140178990
    Abstract: A method for manufacturing at least one kind of eukaryotic cells or a biosynthetic substance derived from the eukaryotic cells by proliferating the cells, which includes preparing an artificially synthesized peptide for promoting proliferation of the at least one kind of eukaryotic cells, incubating the eukaryotic cells in a culture medium, and adding the synthesized peptide at least once to the culture medium during the incubation process. The synthesized peptide includes an amino acid sequence selected from SEQ ID NOS: 1 to 18, and an amino acid sequence selected from SEQ ID NOS: 19 to 97.
    Type: Application
    Filed: January 24, 2014
    Publication date: June 26, 2014
    Applicants: JAPAN TISSUE ENGINEERING CO., LTD., TOAGOSEI CO. LTD.
    Inventors: Tetsuhiko YOSHIDA, Nahoko KOBAYASHI, Mikio NIWA, Chikara SHINOHARA, Masatoki WATANABE, Ken-ichiro HATA
  • Patent number: 8710005
    Abstract: A neuronal differentiation inducer provided by the present invention contains an artificially synthesized peptide which includes an amino acid sequence constituting a signal peptide in amyloid precursor protein (APP), or a partial sequence of the amino acid sequence constituting this signal peptide.
    Type: Grant
    Filed: April 12, 2010
    Date of Patent: April 29, 2014
    Assignee: Toagosei Co., Ltd.
    Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi