Patents by Inventor Nahoko Kobayashi
Nahoko Kobayashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8673846Abstract: A cell proliferation promoter includes, as an active ingredient, an artificially synthesized peptide that includes (A) an amino acid sequence constituting a membrane-permeable peptide and (B) an amino acid sequence selected from SEQ ID NOs: 19 to 103 or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in the selected amino acid sequence.Type: GrantFiled: October 28, 2010Date of Patent: March 18, 2014Assignees: Toagosei Co. Ltd., Japan Tissue Engineering Co., Ltd.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Chikara Shinohara, Masatoki Watanabe, Ken-ichiro Hata
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Patent number: 8673845Abstract: The method for transferring a foreign substance provided by the present invention includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence WRRQARFK (SEQ ID NO. 1) or any amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: GrantFiled: July 28, 2010Date of Patent: March 18, 2014Assignee: Toagosei Co., Ltd.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa, Kenichi Tanaka
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Publication number: 20140051647Abstract: This invention provides a TNFR2 expression-inducing composition including as an active ingredient a peptide having TNFR2 expression-inducing activity, and a method for producing cells that express TNFR2 selectively by use of the composition. The cell production method provided by this invention includes: culturing at least one species of cells capable of expressing TNF receptor 2, and supplying the cells with a synthetic peptide consisting of a nuclear localization signal sequence (NLS) or a nucleolar localization signal sequence (NoLS) to enhance TNFR2 expression in the cells.Type: ApplicationFiled: April 17, 2012Publication date: February 20, 2014Applicant: TOAGOSEI CO. LTD.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa
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Patent number: 8603967Abstract: A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO. 1) or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: GrantFiled: July 28, 2010Date of Patent: December 10, 2013Assignee: Toagosei Co., Ltd.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa
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Publication number: 20130323776Abstract: A method for transferring a foreign substance includes: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including any amino acid sequence selected from SEQ ID NOS: 1-6, or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence of the selected sequence identification number, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 8, 2013Publication date: December 5, 2013Inventors: Tetsuhiko YOSHIDA, Nahoko KOBAYASHI, Mikio NIWA, Kenichi TANAKA
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Publication number: 20130079273Abstract: The pharmaceutical composition includes at least one pharmaceutically acceptable carrier, and an active ingredient including an artificially synthesized peptide includes: (A) an amino acid sequence constituting a cell-penetrating peptide and (B) an amino acid sequence constituting the signal peptide in amyloid precursor protein (APP) or an N-terminal partial amino acid sequence or C-terminal partial amino acid sequence from the amino acid sequence constituting that signal peptide.Type: ApplicationFiled: June 3, 2011Publication date: March 28, 2013Applicant: TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi
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Publication number: 20130005034Abstract: Disclosed is a method for transforming human or non-human mammalian stem cells by introducing a desired peptide motif into the stem cells. The stem cell transformation method disclosed herein uses a synthesized peptide having an amino acid sequence which constitutes the desired peptide motif on the N-terminal end or C-terminal end of a cell membrane-permeable nucleolar localization signal sequence defined by the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO:1).Type: ApplicationFiled: September 12, 2012Publication date: January 3, 2013Applicant: TOAGOSEI CO., LTDInventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Junichi Masuda
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Publication number: 20120208752Abstract: A cell proliferation promoter includes, as an active ingredient, an artificially synthesized peptide that includes (A) an amino acid sequence constituting a membrane-permeable peptide and (B) an amino acid sequence selected from SEQ ID NOs: 19 to 103 or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in the selected amino acid sequence.Type: ApplicationFiled: October 28, 2010Publication date: August 16, 2012Applicants: JAPAN TISSUE ENGINEERING CO., LTD., TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Chikara Shinohara, Masatoki Watanabe, Ken-ichiro Hata
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Publication number: 20120149053Abstract: A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including any amino acid sequence selected from SEQ ID Nos. 1, 2, 3, 4, 5, and 6, or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence of the selected sequence identification number, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 28, 2010Publication date: June 14, 2012Applicant: TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Kenichi Tanaka
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Publication number: 20120122225Abstract: The method for transferring a foreign substance provided by the present invention includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence WRRQARFK (SEQ ID NO. 1) or any amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 28, 2010Publication date: May 17, 2012Applicant: TOAGOSEI CO. LTD.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa, Kenichi Tanaka
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Publication number: 20120122210Abstract: A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO. 1) or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 28, 2010Publication date: May 17, 2012Applicant: TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa
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Patent number: 8138146Abstract: Disclosed is an antiviral agent comprising a non-naturally occurring, artificially synthesized peptide as the main ingredient. The antiviral agent comprises an antiviral peptide, wherein the antiviral peptide has at least one unit of an amino acid sequence constituted by at least five contiguous amino acid residues (which is known as a nuclear localization sequence (NLS)) or an amino acid sequence having a partial modification in the NLS and also having at least one unit of an amino acid sequence constituted by at least five contiguous amino acid residues (which is known as a nuclear export sequence (NES)) or an amino acid sequence having a partial modification in the NES.Type: GrantFiled: February 28, 2007Date of Patent: March 20, 2012Assignee: Toagosei Co., LtdInventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Takanori Sato
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Publication number: 20120035112Abstract: A neuronal differentiation inducer provided by the present invention contains an artificially synthesized peptide which includes an amino acid sequence constituting a signal peptide in amyloid precursor protein (APP), or a partial sequence of the amino acid sequence constituting this signal peptide.Type: ApplicationFiled: April 12, 2010Publication date: February 9, 2012Applicant: Toagosei Co., Ltd.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi
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Patent number: 7964556Abstract: Antimicrobial agent including an artificially synthesized antimicrobial peptide that does not occur naturally is provided by present invention. The peptide included in the antimicrobial agent includes 1 unit, 2 units or more units of sequence(s) or sequence(s) with partial modification, the sequence(s) composed of at least 6 contiguous amino acid residues selected from any one of amino acid sequences: (a) KVQIINKK; (b) SVQIVYKP; (c) QVEVKSEK; (d) KKVAVVRT; (e) KKVAIIRT; (f) KKPTSAK, and the total number of amino acid residues included in 1 unit, 2 units or more units of sequence(s) is 30% or more of the total number of amino acid residues constituting the peptide chain.Type: GrantFiled: June 12, 2007Date of Patent: June 21, 2011Assignee: Toagosei Co., LtdInventors: Nahoko Kobayashi, Tetsuhiko Yoshida
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Patent number: 7893031Abstract: Disclosed is a neuronal differentiation inhibitor which comprises at least one peptide capable of inhibiting or controlling the neuronal differentiation of at least one cell capable of being differentiated into a neuronal cell. The peptide is an artificially synthesized peptide which comprises a BC-box derived amino acid sequence comprising at least 10 contiguous amino acid residues selected from an amino acid sequence constituting the BC-box of at least one protein belonging to the elongin A family or comprises an amino acid sequence having a partial modification in the BC-box derived amino acid sequence.Type: GrantFiled: October 3, 2006Date of Patent: February 22, 2011Assignee: Toagosei Co., Ltd.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Hiroshi Kanno
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Publication number: 20100297758Abstract: The present invention provides a method of transporting a peptide motif of interest into a nucleus of a eukaryotic cell from outside the cell, including: synthesizing a peptide chain having an amino acid sequence constituting the peptide motif of interest at an N-terminal end or a C-terminal end of a cell membrane-permeable nucleolar localization signal sequence defined by the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO: 1); adding the synthesized peptide to a culture medium which includes the eukaryotic cell or a tissue containing the eukaryotic cell; and culturing the eukaryotic cell to which the synthesized peptide is added, or the tissue containing the cell.Type: ApplicationFiled: January 23, 2009Publication date: November 25, 2010Applicant: TOAGOSEI CO., LTDInventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Junichi Masuda
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Patent number: 7615534Abstract: Artificially synthesized antimicrobial peptide that does not occur naturally is provided by the present invention. The antimicrobial peptide includes one unit, two units or more units of amino acid sequence composed of the following 8 amino acid residues: (R or Q)-(L or F or I)—I—K-(L or F or I or V)-L-Y-Q; and/or, modified sequence composed of said sequence with partial modification.Type: GrantFiled: October 11, 2005Date of Patent: November 10, 2009Assignee: Toagosei Co., LtdInventors: Tetsuhiko Yoshida, Yoshinao Yamada, Nahoko Kobayashi, Hiroki Kourai
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Publication number: 20090270334Abstract: Disclosed is a neuronal differentiation inhibitor which comprises at least one peptide capable of inhibiting or controlling the neuronal differentiation of at least one cell capable of being differentiated into a neuronal cell. The peptide is an artificially synthesized peptide which comprises a BC-box derived amino acid sequence comprising at least 10 contiguous amino acid residues selected from an amino acid sequence constituting the BC-box of at least one protein belonging to the elongin A family or comprises an amino acid sequence having a partial modification in the BC-box derived amino acid sequence.Type: ApplicationFiled: October 3, 2006Publication date: October 29, 2009Applicant: TOAGOSEI CO., LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Hiroshi Kanno
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Publication number: 20090258815Abstract: Disclosed is an antiviral agent comprising a non-naturally occurring, artificially synthesized peptide as the main ingredient. The antiviral agent comprises an antiviral peptide, wherein the antiviral peptide has at least one unit of an amino acid sequence constituted by at least five contiguous amino acid residues (which is known as a nuclear localization sequence (NLS)) or an amino acid sequence having a partial modification in the NLS and also having at least one unit of an amino acid sequence constituted by at least five contiguous amino acid residues (which is known as a nuclear export sequence (NES)) or an amino acid sequence having a partial modification in the NES.Type: ApplicationFiled: April 17, 2007Publication date: October 15, 2009Applicant: TOAGOSEI CO., LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Takanori Sato
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Publication number: 20090253618Abstract: A neuronal differentiation inducer comprising at least one peptide which can induce the differentiation of at least one cell into a neurocyte. The peptide is an artificially synthesized peptide comprising an amino acid sequence derived from a BC-box of at least one protein belonging to the SOCS protein family or an amino acid sequence having a partial modification in the BC-box-derived amino acid sequence, wherein the BC-box-derived amino acid sequence is composed of at least 10 contiguous amino acid residues selected in the amino acid sequence constituting the BC-box.Type: ApplicationFiled: July 20, 2006Publication date: October 8, 2009Applicants: TOAGOSEI CO., LTDInventors: Hiroshi Kanno, Tetsuhiko Yoshida, Nahoko Kobayashi