Patents by Inventor Nanibhushan Dattagupta

Nanibhushan Dattagupta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 4950744
    Abstract: A photochemical nucleic acid-labeling reagent of the formula ##STR1## wherein Q is a photoreactive residue of a nucleic acid-binding ligand; L is a detectable label residue; R is hydrogen, C.sub.1 to C.sub.7 -alkyl, aryl, hydroxy, or C.sub.1 to C.sub.7 -alkoxy; x is an integer from 2 through 7; and y is an integer from 3 through 10; wherein R and x, respectively, can be the same or different each time they appear in the formula. The reagent is useful in the highly efficient labeling of nucleic acids for the purpose of detection in hybridization assays.
    Type: Grant
    Filed: March 18, 1987
    Date of Patent: August 21, 1990
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, James P. Albarella
  • Patent number: 4950588
    Abstract: A chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme, a chemiluminescence enhancer and a nitrogen compound selected from the group consisting of ammonia and water-soluble organic amines. The reaction of such process can be used in detection of nucleic acid hybrids, antibodies, antigens and peroxidase enzymes and in producing light.
    Type: Grant
    Filed: September 27, 1988
    Date of Patent: August 21, 1990
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4853327
    Abstract: A chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme and a nitrogen compound selected from the group consisting of ammonia and a water-soluble organic amine. The reaction of such process can be used in detection of nucleic acid hybrids, antibodies, antigens and peroxidase enzymes and in producing light.
    Type: Grant
    Filed: July 10, 1985
    Date of Patent: August 1, 1989
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4824775
    Abstract: In passing labeled cells through a cell sorter, the improvement which comprises employing a labeled cell comprising a cell, an antibody specific to and bound to such cell, a nucleic acid fragment joined to said antibody, and a plurality of labels on said nucleic acid fragment. Because of the presence of multiple labels, the sensitivity of the separation of labeled cells is increased.
    Type: Grant
    Filed: January 3, 1985
    Date of Patent: April 25, 1989
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Michael E. Kamarck
  • Patent number: 4818681
    Abstract: A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to the nucleoside phosphate through its phosphate moiety, in at least one step enzymatically lengthening the mono- or oligonucleotide, cleaving the resultant oligonucleotide from the solid support-nucleoside phosphate at the phosphate moiety of the nucleoside, and separating the oligonucleotide. After cleaving and separating the solid support-nucleoside phosphate is recycled for further coupling. Advantageously the solid support-nucleoside phosphate is phosphorylated between separation and recycling.
    Type: Grant
    Filed: February 22, 1985
    Date of Patent: April 4, 1989
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4808520
    Abstract: A method is provided of making an oligonucleotide which comprises hybridizing (a) a shorter fragment of the desired oligonucleotide with (b) a nucleic acid fragment longer than (a) and complementary to the desired oligonucleotide, one of (a) and (b) is linked to an organic radical which differentiates its physical properties relative to the other of (a) and (b), contacting the hybridized material with an enzyme and nucleoside triphosphates whereby the shorter fragment is extended in one direction until it is substantially coterminal with the complementary nucleic acid fragment, denaturing the hybridized material and separating lengthened (a) from (b).
    Type: Grant
    Filed: March 15, 1985
    Date of Patent: February 28, 1989
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, William J. Knowles
  • Patent number: 4794073
    Abstract: A nucleic acid probe capable of participating in a chemiluminescent reaction comprising a defined nucleic acid sequence, the sequence being linked to any one ofa. a chemiluminescence precursor,b. a chemiluminescence enhancer, andc. an enzyme the remaining two of (a), (b) and (c) not linked to the sequence being in a mixture of the linked sequence. A method for determining a particular single stranded polynucleotide sequence in a test medium, comprising the steps of:(a) combining the test medium with a polynucleotide probe having a base sequence substantially complementary to the sequence to be determined,(b) labeling either the resulting hybrids or probe which has not hybridized with the sequence to be determined with one of the participants in an enhanced chamiluminescent reaction involving a chemiluminescent precursor, an enzyme, an oxidant, and a chemiluminescence enhancer,(c) initiating such chemiluminent reaction with the labeled hybrid or probe, and(d) detecting the resulting light emission.
    Type: Grant
    Filed: July 10, 1985
    Date of Patent: December 27, 1988
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Anton H. Clemens
  • Patent number: 4777129
    Abstract: A nucleic acid detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable, single or double stranded nucleic acid portion, the non-hybridizable portion preferably including a recognition site for binding by a particular protein. Such recognition site can be a region of singly or doubly stranded nucleic acid specific for a particular nucleic acid binding protein such as lac repressor protein or can be a modified nucleic acid region such as a unique antigenic determinant introduced by interaction of the region with a modifier compound such as an intercalating agent or a platinum-containing ligand. The probe-binding protein can be labeled for ease of detection and in the case of an antigenic determinant binding site can be labeled antibody.
    Type: Grant
    Filed: October 19, 1984
    Date of Patent: October 11, 1988
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Peter M. M. Rae, William J. Knowles, Donald M. Crothers
  • Patent number: 4748111
    Abstract: A protein is covalently coupled to a 3'terminal end of a nucleic acid which carries several labels. In an assay the protein will specifically recognize some component of a test system; in an immunoassay the protein can be Protein A which will recognize the FC portion of IgG which is bound to an unknown antigen if present in the test sample.
    Type: Grant
    Filed: March 12, 1984
    Date of Patent: May 31, 1988
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, William J. Knowles, Vincent T. Marchesi, Donald M. Crothers
  • Patent number: 4737454
    Abstract: A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a .beta.-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hybridize.
    Type: Grant
    Filed: May 18, 1984
    Date of Patent: April 12, 1988
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Donald M. Crothers
  • Patent number: 4734363
    Abstract: A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide. The product can be used for making analytical probes.
    Type: Grant
    Filed: November 27, 1984
    Date of Patent: March 29, 1988
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Peter M. M. Rae, Donald M. Crothers, Thomas R. Barnett
  • Patent number: 4724202
    Abstract: A detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable, single or double stranded nucleic acid portion, the non-hybridizable portion preferably including a recognition site for a particular protein.
    Type: Grant
    Filed: December 12, 1983
    Date of Patent: February 9, 1988
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Peter M. M. Rae, William J. Knowles, Donald M. Crothers
  • Patent number: 4713326
    Abstract: A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanedioldiglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.
    Type: Grant
    Filed: May 18, 1984
    Date of Patent: December 15, 1987
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Donald M. Crothers
  • Patent number: 4692509
    Abstract: A radioactively labeled protein comprising a protein, and a radioactive nucleoside or nucleotide, the protein being covalently linked to the nucleoside or nucleotide. Advantageously the linkage is through an NH.sub.2 group of the protein and through a carbonyl group of a ring-opened sugar moiety of the nucleoside or nucleotide. The protein can be insulin, an immunoglobulin or protein A. The radioactive moiety may be a P, C, S, H, I or Hg atom. The labels can be used to indicate the presence and amount of the protein in a biological assay.
    Type: Grant
    Filed: November 27, 1984
    Date of Patent: September 8, 1987
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4670380
    Abstract: In a method for determining a particular polynucleotide sequence in a test medium containing single stranded nucleic acids wherein the sample is subjected to a hybridization reaction with a labeled detection probe having a substantially complementary polynucleotide sequence, and wherein after hybridization the label in said probe is assayed, the improvement wherein the label in said labeled probe comprises a fluorescent nucleotide which is linked by a phosphate ester linkage to said probe. Probes and kits therefor are also provided.
    Type: Grant
    Filed: May 23, 1984
    Date of Patent: June 2, 1987
    Assignee: Molecular Diagnostics, Inc.
    Inventor: Nanibhushan Dattagupta
  • Patent number: 4542102
    Abstract: A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a member selected from the group consisting of a furocoumarin, a phenanthridium halide, and photochemically reactive derivatives thereof, and (c) a divalent radical chemically linking the substrate and the member (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol diglycidyl ether to an amino-substituted angelicin or psoralen or phenanthridinium bromide which in turn is photochemically linked to a nucleic acid. This is capable of hybridizing with other nucleic acid fragments and is thereby useful in diagnostic assays.
    Type: Grant
    Filed: July 5, 1983
    Date of Patent: September 17, 1985
    Assignee: Molecular Diagnostics, Inc.
    Inventors: Nanibhushan Dattagupta, Donald M. Crothers