Abstract: The visualization method of the present invention includes the steps of: visualizing a first substance capable of binding to a target substance in a specimen; and visualizing a second substance produced by action of the first substance in the specimen.

Abstract: The present invention relates to a method for evaluating a drug involved in angiogenesis. The method includes a drug administration step (A) of administering a drug involved in angiogenesis to a subject having a lesion. The method further includes: an imaging step (1) of performing radiography on a subject in a state where metal nanoparticles are present in a blood vessel or an imaging step (2) of performing fluorescence staining and fluorescence imaging using a dimeric protein; and evaluating the drug using an image acquired in the imaging step (1) or (2).

Abstract: There is provided a biological substance quantitation method of quantitating a biological substance in a sample stained with a staining reagent including a fluorescent particle encapsulating a fluorescent substance, based on a fluorescence of the fluorescent substance. The method includes inputting a fluorescent image representing expression of the biological substance in the sample by a fluorescent bright spot; and quantitating an expression amount of the biological substance based on a fluorescence of the fluorescent bright spot. The biological substance is a nucleoprotein expressed at a cell nucleus. The fluorescent particle binds to the biological substance through a primary antibody which is directed against the biological substance as an antigen.

Abstract: There is provided a biological substance quantitation method of quantitating a biological substance in a sample stained with a staining reagent including a fluorescent particle encapsulating a fluorescent substance, based on a fluorescence of the fluorescent substance. The method includes inputting a fluorescent image representing expression of the biological substance in the sample by a fluorescent bright spot; and quantitating an expression amount of the biological substance based on a fluorescence of the fluorescent bright spot. The biological substance is a nucleoprotein expressed at a cell nucleus. The fluorescent particle binds to the biological substance through a primary antibody which is directed against the biological substance as an antigen.

Abstract: An object of the invention is to provide an environmentally-responsive dye-accumulated nanoparticle for grasping an intracellular environment such as pH with respect to each cell. The invention provides an environmentally-responsive dye-accumulated nanoparticle, in which an environmentally-responsive dye is accumulated on the surface of, or inside a thermosetting resin particle, and which has an average particle diameter of 30 to 800 nm, wherein the accumulated amount of the environmentally-responsive dye is 200 to 10,000,000 molecules/particle. The environmentally-responsive dye has dependence on pH, or a Ca ion in terms of fluorescence intensity, and the thermosetting resin is a melamine resin.

Abstract: A quantitative determination method of a biological substance in a sample stained with a staining reagent comprising fluorescent particles each encapsulating a fluorescent substance and binding to a biological substance recognizing site. The method comprises inputting a fluorescent image obtained by photographing the sample, extracting a predetermined region from the fluorescent image to calculate an integrated luminance of the predetermined region, and counting the number of fluorescent particles contained in the predetermined region from the integrated luminance and the average luminance per fluorescent particle.

Abstract: An object of the present invention is to provide a labeling agent which exhibits excellent binding to a target molecule in a bioassay or histological staining method that relates to a compound containing a nitrogen-containing aromatic ring. The labeling agent according to the present invention is a labeling agent having a structure in which a molecular target drug, which is a compound containing a nitrogen atom-containing aromatic ring, is bound with a label via a divalent linking group, wherein one end of the divalent linking group is bound to a carbon atom of the nitrogen atom-containing aromatic ring. The label is preferably a fluorescent substance-integrated nanoparticle, and the nitrogen atom-containing aromatic ring is preferably a pyridine ring.

Abstract: A quantitative determination method of a biological substance in a sample stained with a staining reagent comprising fluorescent particles each encapsulating a fluorescent substance and binding to a biological substance recognizing site. The method comprises inputting a fluorescent image obtained by photographing the sample, extracting a predetermined region from the fluorescent image to calculate an integrated luminance of the predetermined region, and counting the number of fluorescent particles contained in the predetermined region from the integrated luminance and the average luminance per fluorescent particle.

Abstract: An objective is to provide Si/Si3N4 system nanosized particles each exhibiting reduced environmental load together with minimized bio toxicity, excellent chemical stability, enhanced relative light emission intensity, and less degradation of emission intensity during continuous exposure to light; a biosubstance labeling agent capable of keeping on labeling a biosubstance exhibiting luminance enhanced for a long duration with the Si/Si3N4 system nanosized semiconductor particle of the present invention; and also a method of manufacturing the Si/Si3N4 system nanosized semiconductor particle of the present invention.

Abstract: A silica nanoparticle for diagnostic imaging that may be capable of use for X-ray CT, fluorescent imaging, or the like, and have high emission intensity. A method for producing such a silica nanoparticle for diagnostic imaging is provided. Also, a biosubstance labeling agent using the silica nanoparticle for diagnostic imaging is provided. The silica nanoparticle for diagnostic imaging encapsulates an organic fluorescent dye and a metal complex, wherein a ligand of the metal complex form a covalent bond with constituent molecules of the silica nanoparticle.

Abstract: Disclosed are core/shell type semiconductor nanoparticles exhibiting a sufficient emission intensity without causing a blink phenomenon (blinking). The core/shell-type semiconductor nanoparticles have an average particle size of from 2 to 50 nm and comprise an intermediate layer between a core portion and a shell portion, wherein band gap widths of bulk crystals which have the same compositions as those of the core portion, the intermediate portion and the shell portion, respectively, are in the order of: core portion<shell portion<intermediate layer.

Abstract: A fluorescent marker comprising inorganic fluorescent nanoparticles having on a surface of the nanoparticles a modifying group containing a reactive portion, wherein the reactive portion is located at a most remote site of the modifying group from the surface of the nanoparticles; the reactive portion comprises at least one selected from the group consisting of bases consisting of DNA or RNA, nucleotides, polynucleotides and intercalators; and the reactive portion is capable of specifically binding to a living organism.

Abstract: Disclosed are near-infrared light-emitting phosphor nanoparticles with an extremely small particle size, which emit light with a high intensity of emission and which are suitable for a biological substance labeling agent, a method for manufacturing the same, and a biological substance labeling agent employing the same. The near-infrared light-emitting phosphor nanoparticles of the invention are near-infrared light-emitting phosphor nanoparticles with an average particle size of from 2 to 50 nm, which when excited by a near-infrared light with a wavelength in the range of from 700 to 900 nm, emit a near-infrared light with a wavelength in the range of from 700 to 2000 nm, the nanoparticles being characterized in that at least a part of the composition is represented by a specific formula.

Abstract: Disclosed are core/shell type semiconductor nanoparticles exhibiting a sufficient emission intensity without causing a blink phenomenon (blinking). The core/shell-type semiconductor nanoparticles have an average particle size of from 2 to 50 nm and comprise an intermediate layer between a core portion and a shell portion, wherein band gap widths of bulk crystals which have the same compositions as those of the core portion, the intermediate portion and the shell portion, respectively, are in the order of: core portion<shell portion<intermediate layer.

Abstract: This invention provides a semiconductor nanoparticle aggregate comprising three or more types of semiconductor nanoparticles, which are different from each other in diameter, have a narrow particle size distribution, and are different from each other in maximum luminous wavelength of an emission spectrum in a wavelength region of 380 nm to 650 nm, a process for producing the semiconductor nanoparticle aggregate, and a biological substance labeling agent utilizing the semiconductor nanoparticle aggregate. The semiconductor nanoparticle aggregate comprises three or more types of semiconductor nanoparticles which have an identical chemical composition, are different from each other in particle diameter and fall within a particle diameter range of 1.8 to 4 nm and are different from each other in maximum luminous wavelength of an emission spectrum in a wavelength range of 380 to 650 nm.

Abstract: Disclosed is a fluorescent semiconductor particle having a core/shell structure composed of a core particle as a semiconductor particle, and a shell layer by which the core particle is covered, wherein the core particle has a different chemical composition from that of the shell layer; the core particle has an average particle diameter of 1-15 nm and a specific gravity of 1.0-3.0; and the fluorescent semiconductor particle having the core/shell structure has a specific gravity of 0.8-3.

Abstract: Disclosed is a method to produce a semiconductor nanoparticle which is excellent in monodispersibility and productivity. This method is characterized in that two or more kinds of semiconductor nanoparticles having different average particle diameters are dispersed in a solvent and mixed together, and then subjected to size classification.

Abstract: A biomolecule detection reagent comprising semiconductor nanoparticles and magnetic nanoparticles, incorporated in a bead comprising an inorganic compound or an organic polymer, and a surface of the beads is modified with a biomolecule detection molecule.

Abstract: An objective is to provide Si/Si3N4 system nanosized particles each exhibiting reduced environmental load together with minimized bio toxicity, excellent chemical stability, enhanced relative light emission intensity, and less degradation of emission intensity during continuous exposure to light; a biosubstance labeling agent capable of keeping on labeling a biosubstance exhibiting luminance enhanced for a long duration with the Si/Si3N4 system nanosized semiconductor particle of the present invention; and also a method of manufacturing the Si/Si3N4 system nanosized semiconductor particle of the present invention.

Abstract: Disclosed are a phosphor in which prevention of aging deterioration of luminance and prevention of deterioration of discharging characteristics are compatible, a method of manufacturing the phosphor, and a plasma display panel manufactured by using the phosphor. A phosphor of the invention containing Mn as an activator is one with Mn serving as a major emission, wherein a ratio of an activator concentration in a superficial portion of a phosphor particle to an activator concentration inside the phosphor particle is 0.3-0.7.