Patents by Inventor Naomi Sumida

Naomi Sumida has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9771606
    Abstract: The genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region expected to be a UK-2 biosynthetic gene cluster. Moreover, by colony hybridization, DNAs in the region were successfully isolated. Further, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. The strain was found not to produce UK-2. It was verified that the genomic region was the UK-2 biosynthetic gene cluster. Furthermore, Streptoverticillium sp. 3-7 was transformed by introduction of a vector in which the isolated UK-2 biosynthetic gene cluster was inserted. It was also found out that the UK-2 productivity by the transformant was improved about 10 to 60 times or more in comparison with that of the parental strain. Moreover, it was revealed that 2 copies of the UK-2 biosynthetic gene cluster were present per cell in these transformants, respectively.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: September 26, 2017
    Assignee: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Koei Kobayashi, Naomi Sumida, Koji Yanai
  • Publication number: 20160367585
    Abstract: The present invention relates to novel aminoglycoside antibiotics, a process for producing the same, and pharmaceutical use thereof. More specifically, the present invention relates to compounds represented by formula (I), a process for producing the same, and use of the same as antimicrobial agents. wherein R represents amino or hydroxyl.
    Type: Application
    Filed: September 6, 2016
    Publication date: December 22, 2016
    Inventors: Naomi SUMIDA, Koji YANAI, Masato TANI, Takayoshi FUKUSHIMA, Yasumasa OTA, Shuichi GOMI, Akitaka NAKANE
  • Patent number: 9469863
    Abstract: The present invention relates to novel aminoglycoside antibiotics, a process for producing the same, and pharmaceutical use thereof. More specifically, the present invention relates to compounds represented by formula (I), a process for producing the same, and use of the same as antimicrobial agents. wherein R represents amino or hydroxyl.
    Type: Grant
    Filed: December 1, 2008
    Date of Patent: October 18, 2016
    Assignee: MEIJI SEIKA KAISHA, LTD.
    Inventors: Naomi Sumida, Koji Yanai, Masato Tani, Takayoshi Fukushima, Yasumasa Ota, Shuichi Gomi, Akitaka Nakane
  • Publication number: 20160244793
    Abstract: To provide a production method capable of mass production of UK-2 at low cost, the genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region expected to be a UK-2 biosynthetic gene cluster. Moreover, by colony hybridization, DNAs in the region were successfully isolated. Further, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. The strain was found not to produce UK-2. It was verified that the genomic region was the UK-2 biosynthetic gene cluster. Furthermore, Streptoverticillium sp. 3-7 was transformed by introduction of a vector in which the isolated UK-2 biosynthetic gene cluster was inserted. It was also found out that the UK-2 productivity by the transformant was improved about 10 to 60 times or more in comparison with that of the parental strain. Moreover, it was revealed that 2 copies of the UK-2 biosynthetic gene cluster were present per cell in these transformants, respectively.
    Type: Application
    Filed: May 10, 2016
    Publication date: August 25, 2016
    Applicant: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Koei KOBAYASHI, Naomi SUMIDA, Koji YANAI
  • Patent number: 9365879
    Abstract: The genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region expected to be a UK-2 biosynthetic gene cluster. Moreover, by colony hybridization, DNAs in the region were successfully isolated. Further, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. The strain was found not to produce UK-2. It was verified that the genomic region was the UK-2 biosynthetic gene cluster. Furthermore, Streptoverticillium sp. 3-7 was transformed by introduction of a vector in which the isolated UK-2 biosynthetic gene cluster was inserted. It was also found out that the UK-2 productivity by the transformant was improved about 10 to 60 times or more in comparison with that of the parental strain. Moreover, it was revealed that 2 copies of the UK-2 biosynthetic gene cluster were present per cell in these transformants, respectively.
    Type: Grant
    Filed: July 8, 2013
    Date of Patent: June 14, 2016
    Assignee: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Koei Kobayashi, Naomi Sumida, Koji Yanai
  • Patent number: 9090924
    Abstract: There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.
    Type: Grant
    Filed: January 19, 2011
    Date of Patent: July 28, 2015
    Assignee: MEIJI SEIKA PHARMA CO., LTD.
    Inventors: Sato Aihara, Naomi Sumida, Koichiro Murashima, Kouji Yanai, Hiroyuki Anzai, Kentaro Yamamoto
  • Patent number: 8697409
    Abstract: Disclosed are a ketoreductase mutant which can be used for an efficient production of daunorubicin derivatives, a DNA encoding the mutant, a transformant prepared by introducing the DNA thereinto to produce a daunorubicin derivative, and a process of producing a daunorubicin derivative using the transformant. The ketoreductase mutant has an amino acid sequence in which one amino acid residue or two or more amino acid residues selected from the group consisting of amino acids located at positions corresponding to the 42nd, 149th, 153rd, 270th, and 306th amino acids in the amino acid sequence of a ketoreductase (EvaE) from a chlororemomycin-producing bacterium (Amycolatopsis orientalis) are substituted with another amino acid residues.
    Type: Grant
    Filed: May 21, 2010
    Date of Patent: April 15, 2014
    Assignee: Meiji Seika Pharma Co., Ltd.
    Inventors: Fusuke Mazuka, Takayoshi Fukushima, Naomi Sumida, Koji Yanai
  • Publication number: 20140011203
    Abstract: The genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region expected to be a UK-2 biosynthetic gene cluster. Moreover, by colony hybridization, DNAs in the region were successfully isolated. Further, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. The strain was found not to produce UK-2. It was verified that the genomic region was the UK-2 biosynthetic gene cluster. Furthermore, Streptoverticillium sp. 3-7 was transformed by introduction of a vector in which the isolated UK-2 biosynthetic gene cluster was inserted. It was also found out that the UK-2 productivity by the transformant was improved about 10 to 60 times or more in comparison with that of the parental strain. Moreover, it was revealed that 2 copies of the UK-2 biosynthetic gene cluster were present per cell in these transformants, respectively.
    Type: Application
    Filed: July 8, 2013
    Publication date: January 9, 2014
    Inventors: Koei KOBAYASHI, Naomi SUMIDA, Koji YANAI
  • Patent number: 8440400
    Abstract: The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: May 14, 2013
    Assignee: Meiji Seika Pharma Co., Ltd.
    Inventors: Takeshi Murakami, Naomi Sumida, Koji Yanai
  • Publication number: 20130116416
    Abstract: Disclosed are a ketoreductase mutant which can be used for an efficient production of daunorubicin derivatives, a DNA encoding the mutant, a transformant prepared by introducing the DNA thereinto to produce a daunorubicin derivative, and a process of producing a daunorubicin derivative using the transformant. The ketoreductase mutant has an amino acid sequence in which one amino acid residue or two or more amino acid residues selected from the group consisting of amino acids located at positions corresponding to the 42nd, 149th, 153rd, 270th, and 306th amino acids in the amino acid sequence of a ketoreductase (EvaE) from a chlororemomycin-producing bacterium (Amycolatopsis orientalis) are substituted with another amino acid residues.
    Type: Application
    Filed: May 21, 2010
    Publication date: May 9, 2013
    Applicant: MEIJI SEIKA PHARMA, CO., LTD.
    Inventors: Fusuke Mazuka, Takayoshi Fukushima, Naomi Sumida, Koji Yanai
  • Patent number: 8383392
    Abstract: Disclosed is a transformant prepared by introducing a ketoreductase gene involved in the biosynthesis of L-epivancosamine into an actinobacterium originally capable of producing daunorubicin. Also disclosed is a process of efficiently producing a non-natural daunorubicin derivative using the transformant. The transformant is capable of efficiently producing a non-natural daunorubicin derivative such as epidaunorubicin.
    Type: Grant
    Filed: September 12, 2008
    Date of Patent: February 26, 2013
    Assignee: Meiji Seika Pharma Co., Ltd.
    Inventors: Naomi Sumida, Manabu Watanabe, Koji Yanai
  • Publication number: 20130017581
    Abstract: There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene.
    Type: Application
    Filed: January 19, 2011
    Publication date: January 17, 2013
    Inventors: Sato Aihara, Naomi Sumida, Koichiro Murashima, Kouji Yanai, Hiroyuki Anzai, Kentaro Yamamoto
  • Publication number: 20110171692
    Abstract: The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii): (i) DNA encoding a protein selected from the following 1), 2) and 3): 1) a protein consisting of the amino acid sequence of SEQ ID NO: 1, 2) a protein comprising an amino acid sequence which has a deletion, substitution, insertion or addition of one or more amino acids in the amino acid sequence of SEQ ID NO: 1, and 3) a protein comprising an amino acid sequence which has an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1, (ii) DNA consisting of the nucleotide sequence of SEQ ID NO: 2, and (iii) DNA hybridizing to the nucleotide sequence of SEQ ID NO: 2 under stringent conditions.
    Type: Application
    Filed: June 5, 2009
    Publication date: July 14, 2011
    Inventors: Takeshi Murakami, Naomi Sumida, Koji Yanai
  • Publication number: 20110034405
    Abstract: The present invention relates to novel aminoglycoside antibiotics, a process for producing the same, and pharmaceutical use thereof. More specifically, the present invention relates to compounds represented by formula (I), a process for producing the same, and use of the same as antimicrobial agents. wherein R represents amino or hydroxyl.
    Type: Application
    Filed: December 1, 2008
    Publication date: February 10, 2011
    Inventors: Naomi Sumida, Koji Yanai, Masato Tani, Takayoshi Fukushima, Yasumasa Ota, Shuichi Gomi, Akitaka Nakane
  • Publication number: 20100255543
    Abstract: Disclosed is a transformant prepared by introducing a ketoreductase gene involved in the biosynthesis of L-epivancosamine into an actinobacterium originally capable of producing daunorubicin. Also disclosed is a process of efficiently producing a non-natural daunorubicin derivative using the transformant. The transformant is capable of efficiently producing a non-natural daunorubicin derivative such as epidaunorubicin.
    Type: Application
    Filed: September 12, 2008
    Publication date: October 7, 2010
    Applicant: MEIJI SEIKA KAISHA, LTD
    Inventors: Naomi Sumida, Manabu Watanabe, Koji Yanai
  • Patent number: 7790425
    Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: September 7, 2010
    Assignee: Meiji Seika Kaisha, Ltd.
    Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi
  • Publication number: 20100167362
    Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
    Type: Application
    Filed: November 24, 2009
    Publication date: July 1, 2010
    Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi
  • Patent number: 7670803
    Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
    Type: Grant
    Filed: April 3, 2009
    Date of Patent: March 2, 2010
    Assignee: Meji Seika Kaisha, Ltd.
    Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi
  • Publication number: 20090226967
    Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
    Type: Application
    Filed: April 3, 2009
    Publication date: September 10, 2009
    Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi
  • Patent number: 7553640
    Abstract: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
    Type: Grant
    Filed: January 18, 2008
    Date of Patent: June 30, 2009
    Assignee: Meiji Seika Kaisha, Ltd.
    Inventors: Manabu Watanabe, Naoki Mido, Takayoshi Tamura, Naomi Sumida, Takashi Yaguchi