Patents by Inventor Nicolaas Smit

Nicolaas Smit has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190153508
    Abstract: The disclosure provides an asymmetric PCR amplification method for preparation of single-stranded product and primers and kits useful therefor.
    Type: Application
    Filed: November 22, 2017
    Publication date: May 23, 2019
    Applicant: SAFEGUARD BIOSYSTEMS HOLDINGS LTD.
    Inventors: Nicolaas SMIT, Sonja BEDNAR, Holger KLAPPROTH, Kevin BOYNTON
  • Publication number: 20180305740
    Abstract: The present disclosure provides improved methods for bead beating and a bead beating system useful therefor. The bead beating system comprises a sample tube, beads, and a dry blocking agent, and methods for using the bead beating system to extract nucleic acids from cells containing the nucleic acids.
    Type: Application
    Filed: June 29, 2018
    Publication date: October 25, 2018
    Applicant: SAFEGUARD BIOSYSTEMS HOLDINGS LTD.
    Inventors: Holger KLAPPROTH, Nicolaas SMIT
  • Patent number: 10036054
    Abstract: The present disclosure provides improved methods for bead beating and a bead beating system useful therefor. The bead beating system comprises a sample tube, beads, and a dry blocking agent, and methods for using the bead beating system to extract nucleic acids from cells containing the nucleic acids.
    Type: Grant
    Filed: February 14, 2017
    Date of Patent: July 31, 2018
    Assignee: Safeguard Biosystems Holdings Ltd.
    Inventors: Holger Klapproth, Nicolaas Smit
  • Publication number: 20170218356
    Abstract: The present disclosure provides improved methods for bead beating and a bead beating system useful therefor. The bead beating system comprises a sample tube, beads, and a dry blocking agent, and methods for using the bead beating system to extract nucleic acids from cells containing the nucleic acids.
    Type: Application
    Filed: February 14, 2017
    Publication date: August 3, 2017
    Applicant: Safeguard Biosystems Holdings Ltd.
    Inventors: Holger KLAPPROTH, Nicolaas SMIT
  • Publication number: 20100223862
    Abstract: This anchoring system is specifically designed to be used in soft, sandy-like earth to anchor a variety of objects, including recovering of 4×4 vehicles stuck in sand or soft material, that need to be winched out without having a fixed object to anchor it to. In other words no fixed object like a tree or a pole, or another vehicle, is necessary to attach the anchor to. This anchoring system can easily be used by one person. What differentiates this auger from other kinds of augers is that the point of strength is underground and connected to a cable. If the cable is pulled it provides the anchor point to which a tensile load can be applied in a direction oblique to the longitudinal axis of the auger. It can be used in a variety of applications, like anchoring tents and poles, and recovering vehicles etc.
    Type: Application
    Filed: March 6, 2009
    Publication date: September 9, 2010
    Inventor: Jacobus Nicolaas Smit
  • Patent number: 7074610
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: August 30, 2001
    Date of Patent: July 11, 2006
    Assignee: i-Stat Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Jeanne A. Itak, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Susan J. Steiner, Paul Van Der Werf, Henry J. Wieck
  • Publication number: 20020090738
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Application
    Filed: August 30, 2001
    Publication date: July 11, 2002
    Applicant: i-STAT Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Paul Van Der Werf, Henry J. Wieck
  • Patent number: 6306594
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: November 17, 1998
    Date of Patent: October 23, 2001
    Assignee: i-STAT Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Paul Van der Werf, Henry J. Wieck
  • Patent number: 5837454
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 17, 1998
    Assignee: i-STAT Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Imants R. Lauks, Randall M. Mier, deceased, Sylvia Piznik, Nicolaas Smit, Paul Van Der Werf, Henry J. Wieck, Susan Steiner, Jeanne Itak
  • Patent number: 5837446
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over he dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 17, 1998
    Assignee: i-STAT Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Jeanne Itak, Imants R. Lauks, Sylvia Piznik, Nicolaas Smit, Susan Steiner, Paul Van Der Werf, Henry J. Wieck, Randall M. Mier, deceased
  • Patent number: 5554339
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: August 19, 1993
    Date of Patent: September 10, 1996
    Assignee: i-Stat Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Paul Van Der Werf, Henry J. Wieck
  • Patent number: 5466575
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: September 10, 1992
    Date of Patent: November 14, 1995
    Assignee: i-Stat Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Paul Van Der Werf, Henry J. Wieck
  • Patent number: 5200051
    Abstract: An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments.
    Type: Grant
    Filed: November 7, 1989
    Date of Patent: April 6, 1993
    Assignee: I-Stat Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Jeanne A. Itak, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Susan J. Steiner, Paul Van Der Werf, Henry J. Wieck
  • Patent number: 5063081
    Abstract: A plurality of uniform microfabricated sensing devices are produced by establishing a plurality of base sensors on a substrate wafer, forming over at least a portion of each base sensor a permselective layer, superimposing a photoformable proteinaceous photoresist layer over a substantial portion of the permselective layer, and forming a topmost layer of an immobilized ligand receptor. The ligand receptor and corresponding ligand may be immunoreactive species.
    Type: Grant
    Filed: August 15, 1990
    Date of Patent: November 5, 1991
    Assignee: I-Stat Corporation
    Inventors: Stephen N. Cozzette, Graham Davis, Jeanne Itak, Imants R. Lauks, Randall M. Mier, Sylvia Piznik, Nicolaas Smit, Susan Steiner, Paul Van Der Werf, Henry J. Wieck