Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.

Abstract: A firearm sight comprising a generally circular sight support ring surrounding and removably holding an optically clear generally circular lens, said lens having a cylindrical shape, front and back faces, a diameter greater than the thickness of the lens, and a scintillating fiber optic member centrally embedded within said circular lens, a means for mounting said circular sight support ring to a firearm, and a means of adjusting said circular support ring in relation to said means for mounting said ring to a firearm.

Abstract: A firearm sight comprising a generally circular sight support ring surrounding and removably holding an optically clear generally circular lens, said lens having a cylindrical shape, front and back faces, a diameter greater than the thickness of the lens, and a scintillating fiber optic member centrally embedded within said circular lens, a means for mounting said circular sight support ring to a firearm, and a means of adjusting said circular support ring in relation to said means for mounting said ring to a firearm.

Abstract: This invention relates to proteins having an amino acid sequence containing several amino acid subsequences found in nature and wherein at least two different subsequences act as monitor sequences, said subsequences being part of at least one natural protein which is a target protein, wherein the end of each of said two different subsequences have a cleavage site that will be cleaved by the same site-specific proteolytic treatment to release said subsequences.

Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels).

Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample.

Abstract: The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels).

Abstract: The invention relates to a method and apparatus for acquiring, recording and storing information in photographic and electronic media. In particular, the invention relates to low flare image recording and specifically to recording two-dimensional images, such as are found in electrophoretic patterns, along with associated alpha-numeric information. An optical comparator is also disclosed. A method and apparatus of optically and electronically comparing images on film strips is disclosed.

Abstract: The invention relates to a method and apparatus for acquiring, recording and storing information in photographic and electronic media. In particular, the invention relates to low flare image recording and specifically to recording two-dimensional images, such as are found in electrophoretic patterns, along with associated alpha-numeric information. An optical comparator is also disclosed. A method and apparatus of optically and electronically comparing images on film strips is disclosed.

Abstract: A curved-surface cassette/gel system is disclosed in which the walls of a cassette and the major surfaces of a slab-shaped electrophoresis gel in the cassette coact to substantially exclude liquid or gas from between either wall and the major surfaces of the gel. Exclusion is accomplished by exerting a normal force at all points on the walls of the cassette and at all points on the major surfaces of the gel. A curvature may be present in at least one wall of the cassette and in at least one major surface of the cassette. A cassette headpiece may be divided by septa which form an edge seal with the slab gel. The spaces formed between the septa function as wells into which sample materials may be placed.

Abstract: A method and apparatus are provided for electrophoresis of macromolecules. The macromolecules are separated through a tube gel which is connected to an upper electrode buffer reservoir by means of a liquid bridge. The tubes themselves are neither permanently attached nor removably attached to the apparatus but merely rest in and abut appropriate positioning devices. Formation and maintenance of the liquid bridge is promoted by means of hydrophobic surfaces. By balancing the hydrostatic pressure of the upper chamber upon the porous or perforated surface, with the surface tension of the liquid, no leakage from the upper chamber results except by means of the stable liquid bridges.

Abstract: Apparatus for producing a gradient gel which serves as a standard medium for a two-dimensional analysis of proteins, the gel having a density gradient along its height formed by a variation in gel composition, with the apparatus including first and second pumping means each including a plurality of pumps on a common shaft and driven by a stepping motor capable of providing small incremental changes in pump outputs for the gel ingredients, the motors being controlled, by digital signals from a digital computer, a hollow form or cassette for receiving the gel composition, means for transferring the gel composition including a filler tube extending near the bottom of the cassette, adjustable horizontal and vertical arms for automatically removing and relocating the filler tube in the next cassette, and a digital computer programmed to automatically control the stepping motors, arm movements, and associated sensing operations involving the filling operation.

Abstract: Apparatus which includes a plurality of separate weight-responsive, electronic measuring elements, such as load cells, disposed on a base insertable into the bottom of a cash drawer or the like. The drawer has a number of compartments to hold coins of different denominations, a different denomination in each compartment. The cells are disposed one per compartment and are interconnected to an electronic totalizer display unit. The cells have analog signal outputs indicative of the weight of the coins in each of the compartments. The analog signal output in each case is converted to a digital signal output and passes to a totalizer unit providing a digital display of the monetary value of the coins in each compartment, the present monetary total of all compartments, and the change just added to or removed from the drawer. The apparatus includes weighting factor units for adjusting the analog signals to reflect the differences in weight and value of the various coin denominations, for accurate totalizing.

Abstract: A slab-gel loading system includes a prismatic chamber for filling a plurality of slab-gel holders simultaneously. Each slab-gel holder comprises a pair of spaced apart plates defining an intermediate volume for gel containment. The holders are vertically positioned in the chamber with their major surfaces parallel to the chamber end walls. A liquid inlet is provided at the corner between the bottom and a side wall of the chamber for distributing a polymerizable monomer solution or a coagulable colloidal solution into each of the holders. The chamber is rotatably supported so that filling can begin with the corner having the liquid inlet directed downwardly such that the solution is gently funneled upwardly, without mixing, along the diverging side and bottom surfaces. As filling proceeds, the chamber is gradually rotated to position the bottom wall in a horizontal mode.

Abstract: An apparatus is disclosed for simultaneously performing electrophoresis separations on a plurality of slab gels containing samples of protein, protein subunits or nucleic acids. A reservoir of buffer solution is divided into three compartments by two parallel partitions having vertical slots spaced along their length. A sheet of flexible, electrically insulative material is attached to each partition and is provided with vertical slits aligned with the slots. Slab-gel holders are received within the slots with the flexible material folded outwardly as flaps from the slits to overlay portions of the holder surfaces and thereby act as electrical and liquid seals. An elongated, spaghetti-like gel containing a sample of specimen that was previously separated by isoelectric focusing techniques is vertically positioned along a marginal edge portion of the slab gel.