Patents by Inventor Nozomu YACHIE

Nozomu YACHIE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220010373
    Abstract: The present invention provides a method for detecting genome-related information of a cell coexisting with at least one type of test substance, for various types of test substances.
    Type: Application
    Filed: November 7, 2019
    Publication date: January 13, 2022
    Applicants: THE UNIVERSITY OF TOKYO, RIKEN
    Inventors: Nozomu YACHIE, Sadao OTA, Fumiko KAWASAKI
  • Publication number: 20210292752
    Abstract: Disclosed is a method for isolating or identifying target clone cells from a cell population, the method including steps of: preparing a cell population into which a barcode sequence and at least one reporter protein abnormal expression cassette linked to the barcode sequence are introduced; introducing a barcode sequence recognition module targeting an arbitrary barcode sequence and a nucleic acid mutation repair enzyme into cells; repairing a nucleic acid mutation causing abnormal expression occurring in the at least one reporter protein abnormal expression cassette by expression of a complex of the barcode sequence recognition module and the nucleic acid mutation repair enzyme in a cell containing the target barcode sequence, to induce normal expression of the reporter protein; and isolating or identifying target clone cells in which the reporter protein is expressed.
    Type: Application
    Filed: August 13, 2019
    Publication date: September 23, 2021
    Applicants: Spiber Inc., The University of Tokyo
    Inventors: Kana Ishida, Soh Ishiguro, Nozomu Yachie, Chikako Sato, Junichi Sugahara
  • Publication number: 20200279583
    Abstract: An encryption method includes an operation method of an encryption system and is a method of encrypting encryption target information.
    Type: Application
    Filed: September 28, 2018
    Publication date: September 3, 2020
    Inventors: Nozomu Yachie, Junichi Sugahara
  • Publication number: 20200248231
    Abstract: The present invention relates to a method for producing a double-stranded DNA fragment having a desired nucleotide sequence through dual asymmetric PCR (DA-PCR). The method comprises: (1) providing a plurality of oligonucleotides (sense oligonucleotides) each corresponding to a part of a sense strand of the double-stranded DNA fragment and a plurality of oligonucleotides (antisense oligonucleotides) each corresponding to a part of an antisense strand of the double-stranded DNA fragment and mixing together the oligonucleotides with equal concentrations, DNA polymerase, and dNTP to prepare a reaction mixture solution; (2) performing PCR by using the reaction mixture solution from step (1); (3) adding a primer set capable of amplifying the double-stranded DNA fragment of full length to the reaction mixture solution from step (2); and (4) performing PCR by using the reaction mixture solution from step (3).
    Type: Application
    Filed: August 28, 2018
    Publication date: August 6, 2020
    Applicant: Spiber Inc.
    Inventors: Yusuke Ito, Chikako Sato, Nozomu Yachie
  • Publication number: 20200131562
    Abstract: The present invention provides a method for integrally detecting nondestructive measurement information and genome-related information of single cells. More specifically, the present invention uses a method including: preparing a plurality of compartments containing single cell or a derivative thereof, a first bead(s), and a second bead(s) per compartment; detecting both nondestructive measurement information of single cell and imaging information of the first bead(s) and associating the nondestructive measurement information of single cell with the imaging information of the first bead(s) before preparation of each compartment or in each compartment; obtaining a hybridized complex; producing an amplified product derived from the hybridized complex; and integrally detecting nondestructive measurement information and genome-related information in single cell.
    Type: Application
    Filed: May 2, 2018
    Publication date: April 30, 2020
    Applicant: The University of Tokyo
    Inventors: Nozomu YACHIE, Sadao OTA