Patents by Inventor Patricia A. Tekamp-Olson
Patricia A. Tekamp-Olson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7166446Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: GrantFiled: January 28, 2005Date of Patent: January 23, 2007Assignee: Chiron CorporationInventor: Patricia Tekamp-Olson
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Publication number: 20050124042Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: ApplicationFiled: January 28, 2005Publication date: June 9, 2005Applicant: Chiron CorporationInventor: Patricia Tekamp-Olson
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Patent number: 6897043Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: GrantFiled: October 25, 2002Date of Patent: May 24, 2005Assignee: Chiron CorporationInventor: Patricia Tekamp-Olson
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Patent number: 6706496Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: GrantFiled: August 2, 2001Date of Patent: March 16, 2004Assignee: Chiron CorporationInventor: Patricia Tekamp-Olson
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Publication number: 20030077831Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: ApplicationFiled: October 25, 2002Publication date: April 24, 2003Applicant: Chiron CorporationInventor: Patricia Tekamp-Olson
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Publication number: 20020146764Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in eucaryotic cells. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide. In particular, a sequence coding for ubiquitin is joined to another polypeptide of interest providing for high yields of the fusion product.Type: ApplicationFiled: August 16, 2001Publication date: October 10, 2002Applicant: Chiron CorporationInventors: Lawrence S. Cousens, Patricia A. Tekamp-Olson, Jeffrey R. Shuster, James P. Merryweather
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Patent number: 6448379Abstract: Inhibitors, including antibodies, of IL8 binding to its receptors, that interact with the amino-terminal extracellular domain of the IL8 receptor and which compete with IL8 for receptor-binding, are disclosed. The inhibitors are useful modulators of IL8 receptor-mediated biological activity.Type: GrantFiled: September 14, 1993Date of Patent: September 10, 2002Assignee: Chiron CorporationInventors: Patricia Tekamp-Olson, Guy Mullenbach, Mary Ellen Wernette-Hammond
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Patent number: 6436390Abstract: Inhibitors, including antibodies, of IL8 binding to its receptors, that interact with the amino-terminal extracellular domain of the IL8 receptor and which compete with IL8 for receptor-binding, are disclosed. The inhibitors are useful modulators of IL8 receptor-mediated biological activity.Type: GrantFiled: June 7, 1995Date of Patent: August 20, 2002Inventors: Patricia Tekamp-Olson, Guy Mullenbach, Mary Ellen Wernette-Hammond
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Publication number: 20020055169Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: ApplicationFiled: August 2, 2001Publication date: May 9, 2002Applicant: Chiron CorporationInventor: Patricia Tekamp-Olson
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Publication number: 20020028481Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in eucaryotic cells. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide. In particular, a sequence coding for ubiquitin is joined to another polypeptide of interest providing for high yields of the fusion product.Type: ApplicationFiled: May 24, 1995Publication date: March 7, 2002Inventors: LAWRENCE S. COUSENS, PATRICIA A. TEKAMP-OLSON, JEFFREY R. SHUSTER, JAMES P. MERRYWEATHER
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Patent number: 6312923Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: GrantFiled: March 17, 2000Date of Patent: November 6, 2001Assignee: Chiron CorporationInventor: Patricia Tekamp-Olson
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Patent number: 6103234Abstract: An inflammatory cytokine is disclosed which has been isolated from cells that have been incubated with a stimulator material. The inflammatory cytokine comprises a protein that is capable of binding to heparin, inducing localized inflammation characterized by polymorphonuclear cell infiltration when administered subcutaneously and having potent in vitro chemotactic activity while inducing little or no in vitro chemokinesis in polymorphonuclear cells, while lacking the ability to suppress the activity of the anabolic enzyme lipoprotein lipase, cause the cytotoxicity of cachectin/TNF-sensitive cells, stimulate the blastogenesis of endotoxin-resistant C3H/HeJ thymocytes, or induce the production of cachectin/TNF by primary thioglycollate-elicited mouse macrophage cells. A particular inflammatory cytokine has been isolated and its cDNA has been sequenced. The sequence predicts a cDNA of 74 amino acids in length and a molecular weight of 7,908.Type: GrantFiled: June 7, 1995Date of Patent: August 15, 2000Assignee: The Rockefeller UniversityInventors: Stephen D. Wolpe, Anthony Cerami, Barbara Sherry, Patricia A. Tekamp-Olson
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Patent number: 6083723Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: GrantFiled: July 1, 1999Date of Patent: July 4, 2000Assignee: Chiron CorporationInventor: Patricia Tekamp-Olson
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Patent number: 6017731Abstract: Compositions and methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form using a yeast host cell as the expression system are provided. Compositions of the invention are nucleotide sequences encoding a signal peptide sequence for a yeast secreted protein, an optional leader peptide sequence for a yeast secreted protein, a native propeptide leader sequence for a mature protein of interest, and a sequence for the mature protein of interest, all operably linked to a yeast promoter. Each of these elements is associated with a processing site recognized in vivo by a yeast proteolytic enzyme. Any or all of these processing sites may be a preferred processing site that has been modified or synthetically derived for more efficient cleavage in vivo. The compositions are useful in methods for expression of heterologous mammalian proteins and their secretion in the biologically active mature form.Type: GrantFiled: December 12, 1997Date of Patent: January 25, 2000Assignee: Chiron CorporationInventors: Patricia Tekamp-Olson, James P. Merryweather
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Patent number: 5986079Abstract: Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.Type: GrantFiled: June 7, 1995Date of Patent: November 16, 1999Assignees: Genetics Institute, Inc., Chiron CorporationInventors: Philip J. Barr, Anthony J. Brake, Randal J. Kaufman, Patricia Tekamp-Olson, Louise Wasley, Polly A. Wong
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Patent number: 5965425Abstract: Compositions and methods are provided for endopeptidase production and for enhanced efficiencies of processing heterologous precursor polypeptides to mature polypeptides, including proteins requiring gamma-carboxylation for biological activity. These compositions and methods utilize recombinant PACE, a mammalian endopeptidase that is specific for dibasic amino acid sites.Type: GrantFiled: November 8, 1996Date of Patent: October 12, 1999Assignees: Genetics Institute, Inc., Chiron CorporationInventors: Philip J. Barr, Anthony J. Brake, Randal J. Kaufman, Patricia Tekamp-Olson, Louise Wasley, Polly A. Wong
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Patent number: 5866362Abstract: Protein aggregates obtained by a process comprising culturing a host cell that expresses a desired protein in a medium comprising an effective amount of Cu.sup.++ and wherein the desired protein forms inclusion bodies in the host cell.Type: GrantFiled: June 7, 1995Date of Patent: February 2, 1999Assignee: Chiron CorporationInventors: Lawrence S. Cousens, Patricia Tekamp-Olson
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Patent number: 5814484Abstract: Methods for the expression of mammalian MIP-1.alpha. and MIP-1.beta. are disclosed. The methods generally comprise introducing into a yeast cell, a DNA molecule capable of directing the expression and if desired the secretion of either MIP-1.alpha. or MIP-1.beta.. Methods for expression of constructs encoding both MIP-1.alpha. and MIP-1.beta. are also described. The MIP molecules so produced are biologically active.Type: GrantFiled: January 31, 1995Date of Patent: September 29, 1998Inventors: Patricia Tekamp-Olson, Carol Ann Gallegos
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Patent number: 5773223Abstract: A new endothelin B receptor has been identified, and the amino acid and nucleotide sequence of the receptor are provided. The nucleotide sequence is useful to construct expression cassettes and vectors to produce host cells which are capable of expressing the receptor, its mutants, fragments, or fusions. Such polypeptides are useful for identifying new endothelin agonists and antagonists.Type: GrantFiled: September 2, 1993Date of Patent: June 30, 1998Assignee: Chiron CorporationInventors: Venkatakrishna Shyamala, Patricia Tekamp Olson
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Patent number: RE37343Abstract: A yeast &agr;-factor expression system is provided comprised of a truncated leader sequence, containing the &agr;-factor signal peptide and one glycosylation site, linked by a processing site to a non-yeast protein-encoding sequence.Type: GrantFiled: August 27, 1998Date of Patent: August 28, 2001Assignee: Chiron CorporationInventor: Patricia Tekamp-Olson