Patents by Inventor Paul Negulescu
Paul Negulescu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8852881Abstract: A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an “Ussing chamber.Type: GrantFiled: November 29, 2006Date of Patent: October 7, 2014Assignee: Vertex Pharmaceuticals, Inc.Inventors: Paul A. Negulescu, Alec T. Harootunian, Philip E. Salzmann, Javier H. Flores, James E. Sinclair, Minh Vuong, Ashvani K. Singh, Fred F. VanGoor
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Patent number: 8518710Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: April 2, 2012Date of Patent: August 27, 2013Assignee: Life Technologies CorporationInventors: Tom Knapp, Gregory Zlokarnik, Paul Negulescu, Roger Tsien, Timothy Rink
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Patent number: 8163562Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: August 23, 2010Date of Patent: April 24, 2012Assignee: Life Technologies CorporationInventors: Tom Knapp, Gregory Zlokarnik, Paul Negulescu, Roger Tsien, Timothy Rink
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Publication number: 20110076711Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: ApplicationFiled: August 23, 2010Publication date: March 31, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Tom KNAPP, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Timothy James Rink
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Patent number: 7785536Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g. a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: March 5, 2009Date of Patent: August 31, 2010Assignee: Life Technologies CorporationInventors: Tom Knapp, Paul Negulescu, Timothy Rink, Roger Tsien, Gregory Zlokarnik
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Publication number: 20090162926Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g. a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: ApplicationFiled: March 5, 2009Publication date: June 25, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Timothy James Rink
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Publication number: 20070072257Abstract: A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an “Ussing chamber.Type: ApplicationFiled: November 29, 2006Publication date: March 29, 2007Inventors: Paul Negulescu, Alec Harootunian, Philip Salzmann, Javier Flores, James Sinclair, Minh Vuong, Ashvani Singh, Fred VanGoor
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Patent number: 7169609Abstract: A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an “Ussing chamber.Type: GrantFiled: March 31, 2004Date of Patent: January 30, 2007Assignee: Vertex Pharmaceutcals, Inc.Inventors: Paul A. Negulescu, Alec T. Harootunian, Philip E. Salzmann, Javier H. Flores, James E. Sinclair, Minh Vuong, Ashvani K. Singh, Fred F. VanGoor
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Patent number: 7067324Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: January 12, 2001Date of Patent: June 27, 2006Assignee: Invitrogen CorporationInventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Tim Rink
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Publication number: 20060008855Abstract: Disclosed are compositions and methods for their use, such as in identifying G-protein coupled receptors and ligands and compounds that modulate signal transduction. The compositions and methods employ promicuous G-proteins. Activation of the promiscous G-protein can be detected in a variety of assays, including assays in which activation is indicated by a change in fluorescence emission of a sample that contains the composition.Type: ApplicationFiled: September 16, 2005Publication date: January 12, 2006Inventors: Paul Negulescu, Stefan Offermanns, Melvin Simon, Charles Zuker
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Publication number: 20050221274Abstract: A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an “Ussing chamber.Type: ApplicationFiled: March 31, 2004Publication date: October 6, 2005Inventors: Paul Negulescu, Alec Harootunian, Philip Salzmann, Javier Flores, James Sinclair, Minh Vuong, Ashvani Singh, Fred VanGoor
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Publication number: 20050136469Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: ApplicationFiled: January 31, 2005Publication date: June 23, 2005Inventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Tsien, Timothy Rink
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Publication number: 20020025940Abstract: The invention provides for methods and compositions for identifying proteins or chemicals that directly or indirectly modulate a genomic polynucleotide and methods for identifying active genomic polynucleotides. Generally, the method comprises inserting an adeno-associated virus derived expression construct having a reporter gene into an eukaryotic genome, usually non-yeast, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting reporter gene expression in the cell.Type: ApplicationFiled: January 26, 2001Publication date: February 28, 2002Applicant: Aurora Biosciences CorporationInventors: Michael A. Whitney, Kleanthis Xanthopoulos, David Nelson, Paul Negulescu, Frank Craig, J. Gordon Foulkes
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Publication number: 20010006820Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: ApplicationFiled: January 12, 2001Publication date: July 5, 2001Inventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Timothy James Rink
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Patent number: 6221612Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: July 23, 1998Date of Patent: April 24, 2001Assignee: Aurora Biosciences CorporationInventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Tim Rink
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Patent number: 6214563Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents.Type: GrantFiled: July 21, 1998Date of Patent: April 10, 2001Assignee: Aurora Biosciences CorporationInventors: Paul Negulescu, Gregor Zlokarnik, Tom Knapp, Roger Y. Tsien, Tim Rink
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Patent number: 6200762Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention further provides a method of determining bound and free analyte in a sample using at least one photon reducing agent. The present invention also provides a method of screening test chemicals in fluorescent assays using photon reducing agents.Type: GrantFiled: July 17, 1998Date of Patent: March 13, 2001Assignee: Aurora Biosciences CorporationInventors: Gregor Zlokarnik, Paul Negulescu, Tom Knapp, Roger Y. Tsien, Tim Rink
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Patent number: 6004808Abstract: Disclosed are compositions and methods for their use, such as in identifying G-protein coupled receptors and ligands and compounds that modulate signal transduction. The compositions and methods employ promicuous G-proteins. Activation of the promiscous G-protein can be detected in a variety of assays, including assays in which activation is indicated by a change in fluorescence emission of a sample that contains the composition.Type: GrantFiled: June 19, 1997Date of Patent: December 21, 1999Assignee: Aurora BioSciences CorporationInventors: Paul Negulescu, Stefan Offermanns, Melvin Simon, Charles Zuker