Abstract: In some embodiments described herein are methods performed in situ for analyzing chromatin interaction events in a cell or in cells of a sample such as a non-homogenized tissue sample. The methods can comprise the spatial analysis of chromatin interaction events across cell populations in a biological sample. The methods can further comprise obtaining a biological sample, hybridizing probes to target nucleic acid sequences involved in chromatin interaction events and producing a nucleic acid sequence comprising all or part of the target nucleic acid sequences, amplifying the nucleic acid sequence so produced and detecting the amplified nucleic acid sequence in situ.

Abstract: The invention relates to a method which enables the possibility to capture chromosome conformation as well as a kit containing components useful to be used the method, such as to detect promoter and enhancer relations. The method comprises the following steps: i) providing cross-linked genomic DNA, wherein the DNA comprises a first and a second set of regions, ii) fragmenting the cross-linked genome, thus creating a plurality of fragments with junctions, iii) adding a labelled junction marker such as biotin and ligating the fragments to the marker, iv) purifying the marked fragments, v) adding labelled capture probes and selectively purifying the hybridized fragments, and vi) analyzing the fragments captured by hybridization and identify the fragments.

Abstract: The invention relates to a method which enables the possibility to capture chromosome conformation as well as a kit containing components useful to be used the method, such as to detect promoter and enhancer relations. The method comprises the following steps: i) providing cross-linked genomic DNA, wherein the DNA comprises a first and a second set of regions, ii) fragmenting the cross-linked genome, thus creating a plurality of fragments with junctions, iii) adding a labelled junction marker such as biotin and ligating the fragments to the marker, iv) purifying the marked fragments, v) adding labelled capture probes and selectively purifying the hybridised fragments, and vi) analysing the fragments captured by hybridisation and identify the fragments.