Patents by Inventor Preethi Sarvabhowman
Preethi Sarvabhowman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11718836Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: GrantFiled: February 5, 2021Date of Patent: August 8, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Publication number: 20210277462Abstract: The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.Type: ApplicationFiled: May 6, 2021Publication date: September 9, 2021Inventors: Aruna Ayer, Preethi Sarvabhowman, Charles Schwab
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Patent number: 11034999Abstract: The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.Type: GrantFiled: July 8, 2019Date of Patent: June 15, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Aruna Ayer, Preethi Sarvabhowman, Charles Schwab
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Publication number: 20210163904Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: ApplicationFiled: February 5, 2021Publication date: June 3, 2021Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Patent number: 10947516Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: GrantFiled: February 20, 2019Date of Patent: March 16, 2021Assignee: Roche Sequencing Solutions, Inc.Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Publication number: 20210071152Abstract: The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: ApplicationFiled: November 3, 2020Publication date: March 11, 2021Inventors: Evan Amato, Cleoma Arnold, Aruna Ayer, Mara Boenitz-Dulat, Barbara Eckert, Ilya Lederman, Colin McGaw, Preethi Sarvabhowman, Charles Wayan Schwab, Shawn Suko, Eileen Thai, Bigna Woersdoerfer, David Wunderlich
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Patent number: 10851353Abstract: The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: GrantFiled: March 19, 2019Date of Patent: December 1, 2020Assignees: Roche Sequencing Solutions, Inc., Roche Molecular Systems, Inc.Inventors: Evan Amato, Cleoma Arnold, Aruna Ayer, Mara Boenitz-Dulat, Barbara Eckert, Ilya Lederman, Colin McGaw, Preethi Sarvabhowman, Charles Wayan Schwab, Shawn Suko, Eileen Thai, Bigna Woersdoerfer, David Wunderlich
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Patent number: 10829747Abstract: The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: GrantFiled: September 20, 2017Date of Patent: November 10, 2020Assignees: Roche Sequencing Solutions, Inc., Roche Molecular Systems, Inc.Inventors: Aruna Ayer, Cleoma Arnold, Charles Schwab, Preethi Sarvabhowman, Eileen Thai, Ilya Lederman, Colin McGaw, Evan Amato, Barbara Eckert, Shawn Suko, Mara Boenitz-Dulat, Bigna Woersdoerfer, David Wunderlich
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Publication number: 20200190572Abstract: The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: ApplicationFiled: February 26, 2020Publication date: June 18, 2020Inventors: Aruna Ayer, Preethi Sarvabhowman, Dhruti Vasudev Dalal
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Patent number: 10590480Abstract: The present of disclosure provides variant Pol6 polymerase polypeptide, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: GrantFiled: February 27, 2017Date of Patent: March 17, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Aruna Ayer, Preethi Sarvabhowman, Dhruti Vasudev Dalal
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Publication number: 20190376133Abstract: The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.Type: ApplicationFiled: July 8, 2019Publication date: December 12, 2019Applicant: Roche Sequencing Solutions, Inc.Inventors: Aruna Ayer, Preethi Sarvabhowman, Charles Schwab
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Publication number: 20190225952Abstract: The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: ApplicationFiled: March 19, 2019Publication date: July 25, 2019Inventors: Evan Amato, Cleoma Arnold, Aruna Ayer, Mara Boenitz-Dulat, Barbara Eckert, Ilya Lederman, Colin McGaw, Preethi Sarvabhowman, Charles Wayan Schwab, Shawn Suko, Eileen Thai, Bigna Woersdoerfer, David Wunderlich
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Publication number: 20190177706Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: ApplicationFiled: February 20, 2019Publication date: June 13, 2019Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Patent number: 10266813Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: GrantFiled: February 27, 2017Date of Patent: April 23, 2019Assignee: Genia Technologies, Inc.Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Publication number: 20180245147Abstract: The present of disclosure provides variant Pol6 polymerase polypeptide, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result n increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: ApplicationFiled: February 27, 2017Publication date: August 30, 2018Inventors: Aruna Ayer, Preethi Sarvabhowman, Dhruti Vasudev Dalal
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Publication number: 20180094249Abstract: The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.Type: ApplicationFiled: September 20, 2017Publication date: April 5, 2018Inventors: Aruna Ayer, Cleoma Arnold, Charles Schwab, Preethi Sarvabhowman, Eileen Thai, Ilya Lederman, Colin McGaw, Evan Amato, Barbara Eckert, Shawn Suko, Mara BOENITZ-DULAT BOENITZ-DULAT, Bigna WOERSDOERFER, David WUNDERLICH
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Publication number: 20170268052Abstract: The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.Type: ApplicationFiled: February 27, 2017Publication date: September 21, 2017Inventors: Aruna Ayer, Preethi Sarvabhowman, Charles Schwab
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Publication number: 20170267983Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: ApplicationFiled: February 27, 2017Publication date: September 21, 2017Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
