Patents by Inventor Qianshun LI

Qianshun LI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10563205
    Abstract: This invention discloses a nucleic acid aptamer AS1411 modified DNA tetrahedron. The preparation method includes the steps of (1) binding an AS1411 sequence to the 5? terminal of any DNA single-strand in a DNA tetrahedron, synthesizing the DNA, dissolving obtained DNA powder with ddH2O; (2) measuring an absorbance of the DNA and then calculating a total volume of the four single strands; (3) pipetting the DNA obtained in step (1) according to the calculation results in step (2), mixing the DNA with a TM buffer, mixing the mixture uniformly with vortex vibration, and performing a PCR progress. The preparation method is simple. The produced product can effectively solve the problem that the unmodified DNA tetrahedron cannot enter the nucleus and the problem that the AS1411 cannot carry drugs directly.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: February 18, 2020
    Assignee: SICHUAN UNIVERSITY
    Inventors: Yunfeng Lin, Qianshun Li, Xiaoxiao Cai, Shiyu Lin, Xiaoru Shao
  • Patent number: 10507246
    Abstract: This invention discloses a method for preparing TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system, which includes the following steps: binding AS1411 and nucleic acid drug to a tetrahedral DNA nanostructure respectively; selecting four DNA single strands that respectively carry AS1411 and nucleic acid drug; mixing the four DNA single strands; mixing the DNA single strands and the TM buffer uniformly; putting the mixture into a PCR apparatus; raising the temperature to 95° C. quickly and maintaining for 10 min; and next cooling down to 4° C. and maintaining for 20 min to obtain the TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system. This drug delivery system can directly act on cell nucleus and will not be degraded by lysosomal. The targeting specificity is good. The drug can take a good efficacy and the pertinency is high.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: December 17, 2019
    Assignee: Sichuan University
    Inventors: Yunfeng Lin, Sirong Shi, Xiaoxiao Cai, Shiyu Lin, Qianshun LI
  • Publication number: 20190048347
    Abstract: This invention discloses a nucleic acid aptamer AS1411 modified DNA tetrahedron. The preparation method includes the steps of (1) binding an AS1411 sequence to the 5? terminal of any DNA single-strand in a DNA tetrahedron, synthesizing the DNA, dissolving obtained DNA powder with ddH2O; (2) measuring an absorbance of the DNA and then calculating a total volume of the four single strands; (3) pipetting the DNA obtained in step (1) according to the calculation results in step (2), mixing the DNA with a TM buffer, mixing the mixture uniformly with vortex vibration, and performing a PCR progress. The preparation method is simple. The produced product can effectively solve the problem that the unmodified DNA tetrahedron cannot enter the nucleus and the problem that the AS1411 cannot carry drugs directly.
    Type: Application
    Filed: November 30, 2016
    Publication date: February 14, 2019
    Applicant: Sichuan University
    Inventors: Yunfeng LIN, Qianshun LI, Xiaoxiao CAI, Shiyu LIN, Xiaoru SHAO
  • Publication number: 20180344863
    Abstract: This invention discloses a method for preparing TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system, which includes the following steps: binding AS1411 and nucleic acid drug to a tetrahedral DNA nanostructure respectively; selecting four DNA single strands that respectively carry AS1411 and nucleic acid drug; mixing the four DNA single strands; mixing the DNA single strands and the TM buffer uniformly; putting the mixture into a PCR apparatus; raising the temperature to 95° C. quickly and maintaining for 10 min; and next cooling down to 4° C. and maintaining for 20 min to obtain the TDNs-AS1411-nucleic acid drug nanocomposite based drug delivery system. This drug delivery system can directly act on cell nucleus and will not be degraded by lysosomal. The targeting specificity is good. The drug can take a good efficacy and the pertinency is high.
    Type: Application
    Filed: November 21, 2016
    Publication date: December 6, 2018
    Applicant: Sichuan University
    Inventors: Yunfeng LIN, Sirong SHI, Xiaoxiao CAI, Shiyu LIN, Qianshun LI