Abstract: A method and a kit for detecting one or more analytes in a sample is disclosed. In one aspect, the method includes introducing the sample to a surface bound to at least one portion of a first antibody to form a first antibody-analyte complex. The method further includes incubating the first antibody-analyte complex with a set of second antibodies to form a first antibody-analyte-second antibody complex, wherein one second antibody is conjugated with a nucleic acid fragment comprising an exposed 3? hydroxyl group and another second antibody is conjugated with an exposed 5? phosphate group. Additionally, the method includes ligating the nucleic acid fragment comprising the exposed 3? hydroxyl group and the nucleic acid fragment comprising the exposed 5? phosphate group. Furthermore, the method includes separating the ligated nucleic acid fragments from the first antibody-analyte-second antibody complex.