Patents by Inventor Randall W. Nelson

Randall W. Nelson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20100267069
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: June 29, 2010
    Publication date: October 21, 2010
    Inventors: Urban A. Kiernan, Eric E. Niederkofler, Kemmons A. Tubbs, Dobrin Nedelkov, Randall W. Nelson
  • Patent number: 7816095
    Abstract: Presented herein are novel blood plasma/serum biomarkers related to cardiovascular disease. These newly identified biomarkers create the basis for multiple (single) assays using traditional bioassay technologies and when used in combination yield exceptional clinical sensitivity and specificity in the determination of myocardial infarction (MI). A multiplexed, mass spectrometric immunoassay (MSIA) able to simultaneously assay for the new/novel biomarkers as well other MI markers is also presented. Means and methods for evaluating data generated using multiple biomarkers in order to validate findings and further the use of the multiplexed MI assay in clinical, diagnostic and therapeutic uses is also included.
    Type: Grant
    Filed: February 9, 2009
    Date of Patent: October 19, 2010
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Urban A. Kiernan, Dobrin Nedelkov, Randall W. Nelson
  • Publication number: 20100181239
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: December 21, 2009
    Publication date: July 22, 2010
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7635425
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 23, 2007
    Date of Patent: December 22, 2009
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Publication number: 20090215182
    Abstract: Disclosed is a composition that synergistically prevents proteolysis or modification of peptides in sampled biological fluids using sulfonyl fluoride family protease inhibitors at high concentrations combined with at least one additional protease inhibitor of a different type, preferably a broad spectrum protease inhibitor, and a chelator. A preferred embodiment uses AEBSF at 10 mM, Benzamidine at 20 mM and EDTA as the chelator. The disclosed composition may be combined with other protease inhibitors to further modulate its specificity, for instance to additionally target acidic proteases. Additional protease inhibitors, reducing agents, stabilizers and buffering agents may be combined with the disclosed compositions in devices for sampling or testing biological fluids for levels of peptides of interest, or methods therefore. The disclosed devices, compositions and methods are of particular use in sampling and testing for the level of natriuretic peptides.
    Type: Application
    Filed: February 27, 2008
    Publication date: August 27, 2009
    Inventors: Ute Schellenberger, Eric E. Niederkofler, Urban A. Kiernan, Jessica O'Rear, Randall W. Nelson
  • Publication number: 20090209047
    Abstract: Presented herein are novel blood plasma/serum biomarkers related to cardiovascular disease. These newly identified biomarkers create the basis for multiple (single) assays using traditional bioassay technologies and when used in combination yield exceptional clinical sensitivity and specificity in the determination of myocardial infarction (MI). A multiplexed, mass spectrometric immunoassay (MSIA) able to simultaneously assay for the new/novel biomarkers as well other MI markers is also presented. Means and methods for evaluating data generated using multiple biomarkers in order to validate findings and further the use of the multiplexed MI assay in clinical, diagnostic and therapeutic uses is also included.
    Type: Application
    Filed: February 9, 2009
    Publication date: August 20, 2009
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban A. Kiernan, Dobrin Nedelkov, Randall W. Nelson
  • Publication number: 20090197284
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: September 10, 2008
    Publication date: August 6, 2009
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban A. Kiernan, Eric E. Niederkofler, Kemmons A. Tubbs, Dobrin Nedelkov, Randall W. Nelson
  • Publication number: 20080181819
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Application
    Filed: August 23, 2007
    Publication date: July 31, 2008
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Publication number: 20080176333
    Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Application
    Filed: March 26, 2008
    Publication date: July 24, 2008
    Applicant: INTRINSIC BIOPROBES, INC.
    Inventors: Urban A. Kiernan, Eric Niederkofler, Kemmons A. Tubbs, Dobrin Nedelkov, Randall W. Nelson
  • Patent number: 7399641
    Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
    Type: Grant
    Filed: November 11, 2004
    Date of Patent: July 15, 2008
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Randall W. Nelson, Peter Williams, Jennifer Reeve Krone
  • Patent number: 7396687
    Abstract: Presented herein is the construction of pipettor tips (termed MSIA-Tips) containing porous solid supports that are constructed, covalently derivatized with affinity ligand, and used to extract specific proteins and their variants from various biological fluids by repeatedly flowing the fluids through the MSIA-Tips. A second protein species (a mass-shifted variant of the targeted protein doped into the samples at a constant concentration) is co-extracted with the endogenous protein and variants and is used as a quantitative internal reference standard (IRS). Nonspecifcally bound compounds are rinsed from the MSIA-Tip using a series of buffer and water rinses, after which the wild type protein, protein variants and the IRS are eluted from the MSIA-Tips directly onto a target in preparation for analysis such as MALDI-TOF. Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.
    Type: Grant
    Filed: October 4, 2004
    Date of Patent: July 8, 2008
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Randall W. Nelson, Peter Williams, Jennifer Reeve Krone
  • Patent number: 7311826
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 7, 2006
    Date of Patent: December 25, 2007
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7303888
    Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
    Type: Grant
    Filed: July 24, 2006
    Date of Patent: December 4, 2007
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Randall W Nelson, Peter Williams, Jennifer Reeve Krone
  • Patent number: 7087163
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 8, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7087164
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 8, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7087165
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 8, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7083723
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 1, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 7083724
    Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: August 1, 2006
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
  • Patent number: 6974704
    Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.
    Type: Grant
    Filed: March 14, 2001
    Date of Patent: December 13, 2005
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Randall W. Nelson, Peter Williams, Jennifer Reeve Krone
  • Patent number: 6887713
    Abstract: The present invention is directed to a bioactive probe or chip (BC) that allows for the isolation of analytes, such as biomolecules, followed by modification or bioreaction on these said analytes. More specifically, the present invention relates to various methods and apparatuses that include the BC and further include characterization and identification technologies, such as Bioactive Chip Mass Spectrometry (BCMS). Within the context of the present invention, BC provides a method and device for the capture and subsequent modifying, such as digestion or derivatization, of an analyte. Further, real-time information regarding a variety of molecular interactions may be provided by techniques such as interaction analysis (IA). Finally, the variety of molecules are localized and concentrated thereby aiding in the identification and/or quantification of the molecules by techniques such as mass spectroscopy. In one embodiment, a method for performing the modification, or bioreaction, of biomolecules is disclosed.
    Type: Grant
    Filed: February 4, 2003
    Date of Patent: May 3, 2005
    Assignee: Intrinsic Bioprobes, Inc.
    Inventors: Randall W. Nelson, Dobrin Nedelkov