Patents by Inventor René Verwaal

René Verwaal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • A CRISPR-CAS SYSTEM FOR A FILAMENTOUS FUNGAL HOST CELL
    Publication number: 20190194692
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: June 27, 2019
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS, Carsten POHL, Arnold Jacob Mattieu DRIESSEN, Roelof Ary Lans BOVENBERG, Yvonne Irene NYGÅRD
  • AN ASSEMBLY SYSTEM FOR A EUKARYOTIC CELL
    Publication number: 20190185843
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Application
    Filed: December 15, 2016
    Publication date: June 20, 2019
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Blance Elisabeth Maria GIELESEN, Brenda VONK
  • Fermentation process for production of a dicarboxylic acid using fungal cells
    Patent number: 10280439
    Abstract: The present invention relates to a method for a dicarboxylic acid, which method comprises fermenting fungal cells in a vessel comprising a suitable fermentation medium, wherein a least a portion of the fungal cells are reused in the presence of a vitamin and/or a trace element.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: May 7, 2019
    Assignees: DSM IP ASSESTS B.V., ROQUETTE FRERES SA
    Inventors: Mickel Leonardus August Jansen, René Verwaal, Laurent Segueilha, Mélanie Louchart, Tania Veiga Dos Inocentes
  • GUIDE RNA ASSEMBLY VECTOR
    Publication number: 20190055544
    Abstract: The present invention relates relates to a CRISPR-CAS system for a host cell, in particular to a method to produce a circular vector comprising one or more guide-polynucleotide expression cassettes, wherein said one or more guide-polynucleotide expression cassettes comprise a polynucleotide encoding a guide-polynucleotide operably linked to one or more control sequences which direct the expression of said guide-polynucleotide in a host cell, wherein said guide-polynucleotide comprises a guide-sequence that essentially is the reverse complement of a target-polynucleotide in a host cell and wherein the guide-polynucleotide can direct binding of a Cas protein at a target-polynucleotide in a host cell to form a CRISPR-Cas complex, in vivo.
    Type: Application
    Filed: July 6, 2016
    Publication date: February 21, 2019
    Inventors: Paulus Petrus DE WAAL, Johannes Andries ROUBOS, Paul KLAASSEN, René VERWAAL
  • A CRISPR-CAS SYSTEM FOR A LIPOLYTIC YEAST HOST CELL
    Publication number: 20180023096
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 25, 2018
    Applicant: DSM IP ASSETS B.V.
    Inventors: BERNARD MEIJRINK, RENÉ VERWAAL, BIANCA ELISABETH MARIA GIELESEN, JOHANNES ANDRIES ROUBOS
  • A CRISPR-CAS SYSTEM FOR A YEAST HOST CELL
    Publication number: 20180010151
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 11, 2018
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Dicarboxylic acid fermentation process
    Patent number: 9783834
    Abstract: The present invention relates to a process for producing a dicarboxylic acid, comprising fermenting a recombinant fungal cell in a suitable fermentation medium, in the presence of high carbon dioxide concentrations.
    Type: Grant
    Filed: August 24, 2010
    Date of Patent: October 10, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Mickel Leonardus August Jansen, Rene Verwaal
  • Succinic acid production in a eukaryotic cell
    Patent number: 9689005
    Abstract: The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyzes the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.
    Type: Grant
    Filed: October 2, 2013
    Date of Patent: June 27, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Rene Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
  • Process for preparing dicarboxylic acids employing fungal cells
    Patent number: 9624514
    Abstract: The present invention relates to a process for producing a dicarboxylic acid comprising fermenting a fungal cell in a vessel comprising a suitable fermentation medium, comprising adding a gas flow which comprises 20 to 35 v/v % of oxygen and less than 0.1 v/v % of carbon dioxide to the fermentation medium, and maintaining an average partial carbon dioxide pressure of at least about 0.35 bar in the fermentation medium, and producing the dicarboxylic acid.
    Type: Grant
    Filed: July 2, 2012
    Date of Patent: April 18, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Mickel Leonardus August Jansen, Joseph Johannes Heijen, René Verwaal
  • Dicarboxylic acid production in a recombinant yeast
    Patent number: 9556459
    Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: January 31, 2017
    Assignee: DSM IP ASSETS B.V.
    Inventors: Rene Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
  • FERMENTATION PROCESS
    Publication number: 20160194670
    Abstract: The present invention relates to a method for a dicarboxylic acid, which method comprises fermenting fungal cells in a vessel comprising a suitable fermentation medium, wherein a least a portion of the fungal cells are reused in the presence of a vitamin and/or a trace element.
    Type: Application
    Filed: July 18, 2014
    Publication date: July 7, 2016
    Inventors: Mickel Leonardus August JANSEN, René VERWAAL, Laurent SEGUEILHA, Mélanie LOUCHART, Tania VEIGA DOS INOCENTES
  • Dicarboxylic acid production process
    Patent number: 9353387
    Abstract: The present invention relates to a process for the production of a dicarboxylic acid and ethanol which comprises fermenting a genetically modified yeast in a suitable fermentation medium under anaerobic conditions at a pH value of between 1 and 5 and producing the dicarboxylic acid and ethanol. The invention also relates to a process for crystallizing succinic acid from an aqueous solution having a pH of between 1 and 5 and comprising succinic acid, comprising removing part of the aqueous solution by evaporation to obtain a concentrated solution, and bringing the temperature of the concentrated solution to a value of between 10 and 30 degrees Celsius, wherein succinic acid crystals are formed.
    Type: Grant
    Filed: March 23, 2010
    Date of Patent: May 31, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Mickel Leonardus August Jansen, Maarten Job Van de Graaf, René Verwaal
  • Dicarboxylic acid production by fermentation at low pH
    Patent number: 9340805
    Abstract: The present invention relates to a process for the production of a dicarboxylic acid. The process comprises fermenting a yeast in the presence of a carbohydrate-containing substrate and low amounts of oxygen at a pH value at which at least 50% of the dicarboxylic acid is in the acid form. The process of the present invention allows for high yields of the dicarboxylic acid product and is more cost-effective than existing processes in which the salt is produced which during recovery has to be converted to the acid. It also leads to a simpler and more convenient downstream processing.
    Type: Grant
    Filed: March 19, 2015
    Date of Patent: May 17, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: Mickel Leonardus August Jansen, Rene Verwaal
  • Dicarboxylic acid production in eukaryotes
    Patent number: 9340804
    Abstract: The present invention relates to a recombinant eukaryotic microbial cell comprising a nucleotide sequence encoding a heterologous enzyme catalyzing the conversion from phosphoenolpyruvate to oxaloacetate whereby ATP is generated. The invention further relates to a process for the preparation of a dicarboxylic acid such as succinic acid and fumaric acid, comprising fermenting the eukaryotic microbial cell according to the invention in a suitable fermentation medium.
    Type: Grant
    Filed: November 14, 2008
    Date of Patent: May 17, 2016
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
  • DICARBOXYLIC ACID PRODUCTION BY FERMENTATION AT LOW PH
    Publication number: 20150232891
    Abstract: The present invention relates to a process for the production of a dicarboxylic acid. The process comprises fermenting a yeast in the presence of a carbohydrate-containing substrate and low amounts of oxygen at a pH value at which at least 50% of the dicarboxylic acid is in the acid form. The process of the present invention allows for high yields of the dicarboxylic acid product and is more cost-effective than existing processes in which the salt is produced which during recovery has to be converted to the acid. It also leads to a simpler and more convenient downstream processing.
    Type: Application
    Filed: March 19, 2015
    Publication date: August 20, 2015
    Inventors: Mickel Leonardus August JANSEN, Rene VERWAAL
  • Dicarboxylic acid production by fermentation at low pH
    Patent number: 9012187
    Abstract: The present invention relates to a process for the production of a dicarboxylic acid. The process comprises fermenting a yeast in the presence of a carbohydrate-containing substrate and low amounts of oxygen at a pH value at which at least 50% of the dicarboxylic acid is in the acid form. The process of the present invention allows for high yields of the dicarboxylic acid product and is more cost-effective than existing processes in which the salt is produced which during recovery has to be converted to the acid. It also leads to a simpler and more convenient downstream processing.
    Type: Grant
    Filed: May 20, 2009
    Date of Patent: April 21, 2015
    Assignee: DSM IP Assets B.V.
    Inventors: Mickel Leonardus August Jansen, René Verwaal
  • PROCESS FOR PREPARING DICARBOXYLIC ACIDS EMPLOYING FUNGAL CELLS
    Publication number: 20140342416
    Abstract: The present invention relates to a process for producing a dicarboxylic acid comprising fermenting a fungal cell in a vessel comprising a suitable fermentation medium, comprising adding a gas flow which comprises 20 to 35 v/v % of oxygen and less than 0.1v/v % of carbon dioxide to the fermentation medium, and maintaining an average partial carbon dioxide pressure of at least about 0.35 bar in the fermentation medium, and producing the dicarboxylic acid.
    Type: Application
    Filed: July 2, 2012
    Publication date: November 20, 2014
    Applicant: DSM II Assets B.V.
    Inventors: Mickel Leonardus August Jansen, Joseph Johannes Heijnen, Rene Verwaal
  • Dicarboxylic acid production in a recombinant yeast
    Patent number: 8735112
    Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.
    Type: Grant
    Filed: November 14, 2008
    Date of Patent: May 27, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: René Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
  • DICARBOXYLIC ACID PRODUCTION IN A RECOMBINANT YEAST
    Publication number: 20140065680
    Abstract: The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.
    Type: Application
    Filed: November 4, 2013
    Publication date: March 6, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT
  • DICARBOXYLIC ACID PRODUCTION IN A FILAMENTOUS FUNGUS
    Publication number: 20140045230
    Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.
    Type: Application
    Filed: October 21, 2013
    Publication date: February 13, 2014
    Applicant: DSM IP ASSETS B.V.
    Inventors: Rene VERWAAL, Liang WU, Robbertus Antonius DAMVELD, Cornelis Maria Jacobus SAGT