Abstract: Biosensor apparatus and associated method for detecting a target material using a vibrating resonator having a surface that operably interacts with the target material. A detector is in electrical communication with a sensor, the sensor comprising a first paddle assembly connected to a second paddle assembly, the first paddle assembly having at least one microbalance sensing resonator proximate a proximal end and at least one sensing electrical contact proximate a distal end in electrical communication with the sensing resonator. The at least one sensing resonator has a target coating for operably interacting with the target material, and the second paddle assembly has a microbalance reference resonator proximate the proximal end and at least one reference electrical contact proximate the distal end in electrical communication with the reference resonator.

Abstract: Biosensor apparatus and associated method for detecting a target material using a vibrating resonator having a surface that operably interacts with the target material. A detector is in electrical communication with a sensor, the sensor comprising a first paddle assembly connected to a second paddle assembly, the first paddle assembly having at least one microbalance sensing resonator proximate a proximal end and at least one sensing electrical contact proximate a distal end in electrical communication with the sensing resonator. The at least one sensing resonator has a target coating for operably interacting with the target material, and the second paddle assembly has a microbalance reference resonator proximate the proximal end and at least one reference electrical contact proximate the distal end in electrical communication with the reference resonator.

Abstract: Biosensor apparatus and associated method for detecting a target material using a vibrating resonator having a surface that operably interacts with the target material. A detector is in electrical communication with a sensor, the sensor comprising a first paddle assembly connected to a second paddle assembly, the first paddle assembly having at least one microbalance sensing resonator proximate a proximal end and at least one sensing electrical contact proximate a distal end in electrical communication with the sensing resonator. The at least one sensing resonator has a target coating for operably interacting with the target material, and the second paddle assembly has a microbalance reference resonator proximate the proximal end and at least one reference electrical contact proximate the distal end in electrical communication with the reference resonator.

Abstract: A method and system for detecting material bound on a surface of a piezoelectric resonator introduces a signal of a constant frequency through the sensing resonator and detects a change in the insertion phase shift of the resonator as a result of the binding of the material being detected on the surface of the resonator. Environmental effects on the measurement are effectively canceled by the use of a reference resonator driven by the same input signal. A multiple-port sensing device is provided which includes thin-film sensing and reference resonators monolithically formed on a substrate.

Abstract: A system for analysis of specific binding complexes. A reaction surface is treated with a specific receptor chemical (SRC) having specific binding properties and is allowed to bind with an unknown reactant to form specific binding complexes (SRC) on the reaction surface. The output of a laser is directed through the reaction surface to a detection mechanism. Shadows in the detected light may be interpreted to detect the presence of the unknown reactant. The unknown reactant may also be quantified. Preferred embodiments of apparatus, and methods of their use, are disclosed for detecting the presence of the unknown reactant within a system for interpreting the results, including interactive system components by which the bases for interpretation may be updated.

Abstract: Monoclonal antibodies which are heavy-chain specific for porcine Ig's are secreted by novel hybrid cell lines produced by fusing SP2/O myeloma cells with B-lymphocytes from BALB/c mice immunized against porcine Ig's. The monoclonal antibodies are useful in porcine immunological research and pathological diagnosis.