Patents by Inventor Richard F. Selden

Richard F. Selden has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10538804
    Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: January 21, 2020
    Assignee: ANDE Corporation
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Patent number: 10464065
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: November 5, 2019
    Assignee: ANDE Corporation
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20190019290
    Abstract: Described herein is an adaptive expert system comprising a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions. The adaptive expert system receives sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA. The adaptive expert system determines at least one characteristic of the sample data. The adaptive expert system utilizes the at least one characteristic to classify the sample data and apply a predefined parameter set to the said sample data to generate an output.
    Type: Application
    Filed: July 12, 2018
    Publication date: January 17, 2019
    Inventors: Eugene Tan, Hua Jiang, Richard F. Selden
  • Publication number: 20180230451
    Abstract: Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.
    Type: Application
    Filed: February 15, 2018
    Publication date: August 16, 2018
    Inventors: Richard F. Selden, Rosemary Turingan Witkowski
  • Publication number: 20180169660
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Application
    Filed: February 12, 2018
    Publication date: June 21, 2018
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Patent number: 9889449
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: February 13, 2018
    Assignee: ANDE Corporation
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Publication number: 20180031481
    Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.
    Type: Application
    Filed: October 16, 2017
    Publication date: February 1, 2018
    Inventors: James W. Schumm, Richard F. Selden, Eugene Tan
  • Patent number: 9797841
    Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: October 24, 2017
    Assignee: ANDE Corporation
    Inventors: James W. Schumm, Richard F. Selden, Eugene Tan
  • Publication number: 20170274381
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Application
    Filed: December 2, 2016
    Publication date: September 28, 2017
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20170260567
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Application
    Filed: January 26, 2017
    Publication date: September 14, 2017
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20170225164
    Abstract: A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.
    Type: Application
    Filed: November 14, 2016
    Publication date: August 10, 2017
    Inventors: Richard F. Selden, Eugene Tan, Heung Chuan Lam, Heidi Susanne Giese, Gregory John Kellogg, John A. Wright
  • Publication number: 20170204453
    Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.
    Type: Application
    Filed: January 19, 2017
    Publication date: July 20, 2017
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Publication number: 20170144157
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Application
    Filed: October 3, 2016
    Publication date: May 25, 2017
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20170100718
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Application
    Filed: May 10, 2016
    Publication date: April 13, 2017
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Publication number: 20170087552
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Application
    Filed: May 2, 2016
    Publication date: March 30, 2017
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 9550985
    Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: January 24, 2017
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Richard F Selden, Rosemary S. Turingan
  • Patent number: 9494519
    Abstract: A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.
    Type: Grant
    Filed: March 3, 2011
    Date of Patent: November 15, 2016
    Assignee: NetBio, Inc.
    Inventors: Richard F. Selden, Eugene Tan, Heung Chuan Lam, Heidi Susanne Giese, Gregory John Kellogg, John A. Wright
  • Patent number: 9366631
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Grant
    Filed: July 27, 2011
    Date of Patent: June 14, 2016
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Patent number: 9354199
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: March 9, 2011
    Date of Patent: May 31, 2016
    Assignee: NetBio, Inc.
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 9314795
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: April 19, 2016
    Assignee: NetBio Inc.
    Inventors: Richard F. Selden, Eugene Tan