Patents by Inventor Richard F. Selden

Richard F. Selden has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240271225
    Abstract: Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.
    Type: Application
    Filed: December 20, 2023
    Publication date: August 15, 2024
    Inventors: Richard F. Selden, Rosemary Turingan Witkowski
  • Patent number: 11900604
    Abstract: Described herein is an adaptive expert system comprising a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions. The adaptive expert system receives sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA. The adaptive expert system determines at least one characteristic of the sample data. The adaptive expert system utilizes the at least one characteristic to classify the sample data and apply a predefined parameter set to the said sample data to generate an output.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: February 13, 2024
    Assignee: ANDE Corporation
    Inventors: Eugene Tan, Hua Jiang, Richard F. Selden
  • Publication number: 20230394665
    Abstract: Described herein is an adaptive expert system comprising a computing device having a memory that stores programmatic instructions and a processor that executes the programmatic instructions. The adaptive expert system receives sample data comprising at least one of raw data, optical data, and electropherogram data from a DNA analysis device, said data generated from a sample containing DNA. The adaptive expert system determines at least one characteristic of the sample data. The adaptive expert system utilizes the at least one characteristic to classify the sample data and apply a predefined parameter set to the said sample data to generate an output.
    Type: Application
    Filed: August 17, 2023
    Publication date: December 7, 2023
    Applicant: Ande Corporation
    Inventors: Eugene Tan, Hua Jiang, Richard F. Selden
  • Publication number: 20230256451
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Application
    Filed: February 21, 2023
    Publication date: August 17, 2023
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 11612893
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: March 28, 2023
    Assignee: ANDE CORPORATION
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 11612894
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: March 28, 2023
    Assignee: ANDE CORPORATION
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20220389487
    Abstract: Described herein are instruments for excitation and detection of fluorophores in a plurality of functional regions in a biochip, using an excitation source and a steering element that directs a beam from the excitation source to a plurality of functional regions in the biochip, wherein the excitation source excites the fluorophores in the plurality of functional regions generating a signal that is detected such that said signal from at least one of the plurality of functional regions allows for nucleic acid quantification. Also described are systems for quantification and separation and detection using optical devices adapted for preliminary, simultaneous or sequential quantitation of nucleic acid in separate detection positions, and for the excitation and detection of multiple samples to steer both the excitation and detection beam paths to separately image each lane of a biochip.
    Type: Application
    Filed: August 8, 2022
    Publication date: December 8, 2022
    Applicant: ANDE Corporation
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Publication number: 20220315996
    Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.
    Type: Application
    Filed: June 9, 2022
    Publication date: October 6, 2022
    Applicant: ANDE CORPORATION
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Patent number: 11441173
    Abstract: Described herein are instruments for excitation and detection of fluorophores in a plurality of functional regions in a biochip, using an excitation source and a steering element that directs a beam from the excitation source to a plurality of functional regions in the biochip, wherein the excitation source excites the fluorophores in the plurality of functional regions generating a signal that is detected such that said signal from at least one of the plurality of functional regions allows for nucleic acid quantification. Also described are systems for quantification and separation and detection using optical devices adapted for preliminary, simultaneous or sequential quantitation of nucleic acid in separate detection positions, and for the excitation and detection of multiple samples to steer both the excitation and detection beam paths to separately image each lane of a biochip.
    Type: Grant
    Filed: December 27, 2019
    Date of Patent: September 13, 2022
    Assignee: ANDE CORPORATION
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Publication number: 20220177980
    Abstract: Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.
    Type: Application
    Filed: July 29, 2019
    Publication date: June 9, 2022
    Inventors: Richard F. Selden, Rosemary Turingan Witkowski
  • Publication number: 20220017889
    Abstract: Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.
    Type: Application
    Filed: October 1, 2021
    Publication date: January 20, 2022
    Inventors: Richard F. Selden, Rosemary Turingan Witkowski
  • Patent number: 11168318
    Abstract: Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: November 9, 2021
    Assignee: ANDE CORPORATION
    Inventors: Richard F. Selden, Rosemary Turingan Witkowski
  • Patent number: 11110461
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Grant
    Filed: February 12, 2018
    Date of Patent: September 7, 2021
    Assignee: ANDE CORPORATION
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Patent number: 11022555
    Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.
    Type: Grant
    Filed: October 16, 2017
    Date of Patent: June 1, 2021
    Assignee: ANDE CORPORATION
    Inventors: James W. Schumm, Richard F. Selden, Eugene Tan
  • Patent number: 10821445
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: November 3, 2020
    Assignee: ANDE Corporation
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20200248243
    Abstract: Described herein are instruments for excitation and detection of fluorophores in a plurality of functional regions in a biochip, using an excitation source and a steering element that directs a beam from the excitation source to a plurality of functional regions in the biochip, wherein the excitation source excites the fluorophores in the plurality of functional regions generating a signal that is detected such that said signal from at least one of the plurality of functional regions allows for nucleic acid quantification. Also described are systems for quantification and separation and detection using optical devices adapted for preliminary, simultaneous or sequential quantitation of nucleic acid in separate detection positions, and for the excitation and detection of multiple samples to steer both the excitation and detection beam paths to separately image each lane of a biochip.
    Type: Application
    Filed: December 27, 2019
    Publication date: August 6, 2020
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Publication number: 20200215544
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Application
    Filed: October 7, 2019
    Publication date: July 9, 2020
    Inventors: Richard F. Selden, Eugene Tan
  • Publication number: 20200070173
    Abstract: A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.
    Type: Application
    Filed: May 14, 2019
    Publication date: March 5, 2020
    Inventors: Richard F. Selden, Eugene Tan, Heung Chuan Lam, Heidi Susanne Giese, Gregory John Kellogg, John A. Wright
  • Patent number: 10538804
    Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: January 21, 2020
    Assignee: ANDE Corporation
    Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
  • Patent number: 10464065
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: November 5, 2019
    Assignee: ANDE Corporation
    Inventors: Richard F. Selden, Eugene Tan