Patents by Inventor Richard Kerkman

Richard Kerkman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20150218221
    Abstract: The present invention relates to a method for producing a cyclic peptide compound or a salt thereof which comprises contacting a cyclic lipopeptide compound or a salt thereof with an acylase derived from a microorganism belonging to the genus Pseudomonas to deacylate the lipid acyl moiety of said cyclic lipopeptide.
    Type: Application
    Filed: September 20, 2013
    Publication date: August 6, 2015
    Inventors: Jan Metske Van Der Laan, Richard Kerkman, Rudolf Gijsbertus Marie Luiten, Marco Alexander Van Den Berg
  • Publication number: 20140303036
    Abstract: The present invention relates to a method for the preparation of a library of host cells, a plurality of which comprise an assembled polynucleotide at a target locus, which method comprises: (a) providing a plurality of polynucleotides comprising two or more polynucleotide subgroups, wherein: (i) a plurality of polynucleotides in each polynucleotide subgroup comprises sequence encoding a peptide or polypeptide and/or a regulatory sequence; (ii) a plurality of peptides or polypeptides encoded by, or a plurality of regulatory sequences comprised within, each polynucleotide subgroup share an activity and/or function; (iii) at least one polynucleotide subgroup comprises at least two non-identical polynucleotide species; (iv) a plurality of polynucleotides of each polynucleotide subgroup comprises sequence enabling homologous recombination with a plurality of polynucleotides from one or more other polynucleotide subgroups; and (v) a plurality of polynucleotides in two polynucleotide subgroups comprise a nucleotide
    Type: Application
    Filed: November 23, 2012
    Publication date: October 9, 2014
    Inventors: Johannes Andries Roubos, Bernard Meijrink, Richard Kerkman, Ben Den Dulk
  • Patent number: 8541199
    Abstract: The present invention relates to a mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant is having an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid positions A3, A77, A90, A144 A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the Escherichia coli penicillin G acylase having the amino acid sequence depicted in SEQ ID No: 1.
    Type: Grant
    Filed: December 22, 2009
    Date of Patent: September 24, 2013
    Assignee: DSM Sinochem Pharmaceuticals Netherlands B.V.
    Inventors: Jan Metske Van Der Laan, Harold Monro Moody, Richard Kerkman, Thomas Van Der Does
  • Publication number: 20120282698
    Abstract: A method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a desired replacement sequence is disclosed.
    Type: Application
    Filed: May 11, 2012
    Publication date: November 8, 2012
    Applicant: DSM IP ASSETS B.V.
    Inventors: Marco Alexander van den BERG, Richard KERKMAN, Hesselien TOUW-RIEL
  • Publication number: 20090170206
    Abstract: We disclose a method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a desired replacement sequence, comprising: modifying a parent eukaryotic cell with a preference for NHR to provide a eukaryotic cell having an increased HR/NHR ratio as compared to the parent cell, providing a DNA molecule comprising a first DNA fragment comprising a desired replacement sequence flanked at its 5? and 3? sides by DNA sequences substantially homologous to sequences of the chromosomal DNA flanking the target sequence and a second DNA fragment comprising an expression cassette comprising a gene encoding a selection marker operably linked to regulatory sequences functional in the eukaryotic cell, transforming the modified eukaryotic cells with the DNA molecule, selecting transformed progeny cells having the DNA molecule inserted into the chromosome, deselecting transformed progeny cells having the DNA molecule inserted into the chromosome via NHR by expression of the selection mar
    Type: Application
    Filed: March 14, 2007
    Publication date: July 2, 2009
    Inventors: Marco Alexander van den Berg, Richard Kerkman, Hesselien Touw-Riel
  • Patent number: 7402383
    Abstract: The present invention discloses a process for the preparation of variant polynucleotides using a combination of mutagenesis of a starting population of polynucleotides and recombination of the mutated polynucleotides. The process comprises the steps of subjecting a population of polynucleotides to (a series of) two (or more) separate PCR's comprising a first PCR with a forward mutation-specific primer for a position to be mutated and a reverse universal primer and a second PCR with a forward universal primer and a reverse mutation-specific primer for a position to be mutated. The products of the (series of) two (or more) PCR's are assembled by a polymerase, preferably in one tube.
    Type: Grant
    Filed: July 23, 2002
    Date of Patent: July 22, 2008
    Assignee: DSM IP Assets B.V.
    Inventors: Roelof Ary Lans Bovenberg, Richard Kerkman
  • Publication number: 20050130140
    Abstract: The present invention discloses a process for the preparation of variant polynucleotides using a reassembly process of preferably blunt-ended restriction enzyme fragments prepared form a starting population of heterologous polynucleotides in the presence of a thermostable ligase.
    Type: Application
    Filed: July 23, 2002
    Publication date: June 16, 2005
    Inventors: Roelof Bovenberg, Richard Kerkman
  • Publication number: 20050130141
    Abstract: The present invention discloses a process for the preparation of variant polynucleotides using a combination of mutagenesis of a starting population of polynucleotides and recombination of the mutated polynucleotides. The process comprises the steps of subjecting a population of polynucleotides to (a series of) two (or more) separate PCR's comprising a first PCR with a forward mutation-specific primer for a position to be mutated and a reverse universal primer and a second PCR with a forward universal primer and a reverse mutation-specific primer for a position to be mutated. The products of the (series of) two (or more) PCR's are assembled by a polymerase, preferably in one tube.
    Type: Application
    Filed: July 23, 2002
    Publication date: June 16, 2005
    Inventors: Roelof Bovenberg, Richard Kerkman
  • Patent number: 6692951
    Abstract: Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.
    Type: Grant
    Filed: May 17, 2002
    Date of Patent: February 17, 2004
    Assignee: Genencor International, Inc.
    Inventors: Wilhelmus J. Quax, Richard Kerkman, Cornelis P. Broekhuizen
  • Publication number: 20030077729
    Abstract: Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.
    Type: Application
    Filed: May 17, 2002
    Publication date: April 24, 2003
    Inventors: Wilhelmus J. Quax, Richard Kerkman, Cornelis P. Broekhuizen
  • Patent number: 6410262
    Abstract: Heterologous protein secretion from Gram-positive bacteria, in particular from Bacilli has, with few exceptions, met with little success. Incompatibility of the heterologous proteins with the protein secretion machinery of the host is the main cause of this effect. This limiting factor for the production of heterologous proteins in commercially significant concentrations from Bacillus subtilis is removed by overexpressing the Bacillus subtilis protein FtsY or FtsY protein in combination with overexpression of other members of the bacterial signal recognition particle. Said gene(s) is(are) overexpressed in Bacillus host cells expressing a heterologous protein which then shows an increased amount of the heterologous protein secreted in the surrounding medium.
    Type: Grant
    Filed: December 16, 1997
    Date of Patent: June 25, 2002
    Assignee: Genencor International, Inc.
    Inventors: Wilhelmus J. Quax, Richard Kerkman, Cornelis P. Broekhuizen
  • Patent number: 6368820
    Abstract: An improved process for production of an N-acylated cephalosporin is described. The process utilizes an Acremonium strain which has been modified to express an acyl transferase of a filamentous fungus and is selected for production of the N-acylated cephalosporin at a level which is equal to or higher than the production of the corresponding &agr;-aminoadipyl-7-cephalosporins in the strain.
    Type: Grant
    Filed: January 15, 1999
    Date of Patent: April 9, 2002
    Assignee: DSM N.V.
    Inventors: Roelof Ary Lans Bovenberg, Dirk Schipper, Richard Kerkman