Abstract: A method for forming a prosthesis comprising a bone-like portion and a cartilage-like portion can comprise additively manufacturing a first positive mold in accordance with a portion of a first three-dimensional model of a portion of a bone. A first negative mold can be formed from the first positive mold. The bone-like portion can be created within the first negative mold. A second positive mold of the bone and a cartilage can be additively manufactured from a second three-dimensional model. A portion of the second three-dimensional model can correspond to a portion of the first three-dimensional model. A second negative mold can be formed from the second positive mold. The bone-like portion can be positioned in the second negative mold so that the second negative mold and the bone-like portion can define a cartilage space that can be filled with a material to form the cartilage-like portion of the prosthesis.

Abstract: The present disclosure is concerned with magneto-patterned cell-laden hydrogel materials and methods of making and using those materials. The disclosed materials are useful for, among other things, repair of tissue defects, e.g., tissue at a tissue interface such as a bone-cartilage interface.

Abstract: A method for forming a prosthesis comprising a bone-like portion and a cartilage-like portion can comprise additively manufacturing a first positive mold in accordance with a portion of a first three-dimensional model of a portion of a bone. A first negative mold can be formed from the first positive mold. The bone-like portion can be created within the first negative mold. A second positive mold of the bone and a cartilage can be additively manufactured from a second three-dimensional model. A portion of the second three-dimensional model can correspond to a portion of the first three-dimensional model. A second negative mold can be formed from the second positive mold. The bone-like portion can be positioned in the second negative mold so that the second negative mold and the bone-like portion can define a cartilage space that can be filled with a material to form the cartilage-like portion of the prosthesis.

Abstract: Disclosed are engineered vertebral disc implants comprising an engineered vertebral disc, wherein the engineered vertebral disc comprises a nucleus pulposus region and an annulus fibrosus region; and two endplates, wherein the endplates comprise a porous polymer foam, and wherein the endplates comprise channels. Disclosed are methods of treating disc degeneration comprising implanting one or more of the disclosed engineered vertebral disc implants to a subject in need thereof, wherein the endplates of the engineered vertebral disc implant are attached to the vertebra of the subject.

Abstract: The invention relates to the field of storage of tissues, such as transplantable material, or allograft storage, and more specifically to the field of both short- and long-term tissues storage and preservation. Preferably the tissues are transplantable material comprises chondogenic cells, such as chondrocytes, cartilage, engineered cartilage or to osteochondral/cartilage explants or fragment thereof.

Abstract: Embodiments of the present invention relate to mechanically-activated microcapsules (MAMCs) for controlled drug-delivery, wherein the MAMCs release one or more active ingredients in response to mechanical stimuli in a subject's body. The MAMCs provide a platform for stimulating biological regeneration, biological repair, modifying disease, and/or controlling disease in mechanically-loaded musculoskeletal tissues.

Abstract: Provided are materials comprising layers of anisotropically aligned fibers, the alignment of which fibers may be adjusted so as to give rise to circumferentially-aligned fibers that replicate the fiber alignment of native fibrous tissue, such as the meniscus or the annulus fibrosis. Also provided are laminates formed from the disclosed materials, as well as methods of fabricating the disclosed materials and laminates.

Abstract: Provided are implant scaffolds comprising angle-ply arrays of two or more layers of substantially aligned fiber, methods of making and using said scaffolds, and kits comprising such scaffolds.

Abstract: A bioreactor generates load-bearing cartilaginous or fibro-cartilaginous tissue by applying hydrostatic pressure and/or deformational loading to scaffolds seeded with chondrocytes and/or other cells. A scaffold may be shaped to reproduce the geometry of all or part of a load bearing articular surface or defect as acquired from a database or patient-specific geometry data. Optionally a scaffold can be attached to a substrate which promotes integration of this tissue construct with the underlying bone of the patient joint. In the bioreactor, ambient hydrostatic pressure and scaffold deformational loading can be prescribed with any desired waveform, using magnitudes which prevail in diarthrodial joints. The loading platen, permeable or impermeable, may conform to all or part of the scaffold surfaces.