Patents by Inventor Robert W. Blakesley
Robert W. Blakesley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 7833705Abstract: The invention relates to a solid medium or matrix for storage of nucleic acid molecules (e.g. RNA and/or DNA), particularly vectors and especially plasmids, comprising a solid matrix preferably having a compound or composition which protects against degradation of nucleic acids incorporated into or absorbed on the matrix. The invention also relates to methods for storage or isolation/purification of nucleic acids using this solid medium, and in situ use of the stored or isolated/purified nucleic acids.Type: GrantFiled: June 19, 2003Date of Patent: November 16, 2010Assignee: Whatman, Inc.Inventors: Robert W. Blakesley, Mindy D. Goldsborough
-
Patent number: 7344835Abstract: Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate are disclosed. More specifically, the present invention concerns inhibiting or preventing pyrophosphorolysis in sequencing and amplification of nucleic acid molecules. According to the present invention, an enzyme which is a pentosyltransferase, a phosphotransferase with an alcohol group as acceptor, a nucleotidyltransferase, or a carboxy-lyase is added to the reaction which serves to remove pyrophosphate from the reaction mixture.Type: GrantFiled: June 7, 2004Date of Patent: March 18, 2008Assignee: Invitrogen CorporationInventor: Robert W. Blakesley
-
Patent number: 6764839Abstract: Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate are disclosed. More specifically, the present invention concerns inhibiting or preventing pyrophosphorolysis in sequencing and amplification of nucleic acid molecules. According to the present invention, an enzyme which is a pentosyltransferase, a phosphotransferase with an alcohol group as acceptor, a nucleotidyltransferase, or a carboxy-lyase is added to the reaction which serves to remove pyrophosphate from the reaction mixture.Type: GrantFiled: June 19, 2001Date of Patent: July 20, 2004Assignee: Invitrogen CorporationInventor: Robert W. Blakesley
-
Patent number: 6750059Abstract: The invention relates to a solid medium or matrix for storage of nucleic acid molecules (e.g. RNA and/or DNA), particularly vectors and especially plasmids, comprising a solid matrix preferably having a compound or composition which protects against degradation of nucleic acids incorporated into or absorbed on the matrix. The invention also relates to methods for storage or isolation/purification of nucleic acids using this solid medium, and in situ use of the stored or isolated/purified nucleic acids.Type: GrantFiled: July 16, 1999Date of Patent: June 15, 2004Assignee: Whatman, Inc.Inventors: Robert W. Blakesley, Mindy D. Goldsborough
-
Publication number: 20040028664Abstract: The invention relates to a solid medium or matrix for storage of nucleic acid molecules (e.g. RNA and/or DNA), particularly vectors and especially plasmids, comprising a solid matrix preferably having a compound or composition which protects against degradation of nucleic acids incorporated into or absorbed on the matrix. The invention also relates to methods for storage or isolation/purification of nucleic acids using this solid medium, and in situ use of the stored or isolated/purified nucleic acids.Type: ApplicationFiled: June 19, 2003Publication date: February 12, 2004Applicants: Life Technologies, Inc., Whatman, Inc.Inventors: Robert W. Blakesley, Mindy D. Goldsborough
-
Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
Publication number: 20020012982Abstract: The present invention relates generally to compositions, methods and kits for use in extracting and isolating protein or peptide molecules. More specifically, the invention relates to such compositions, methods and kits that are useful in the isolation of protein or peptide molecules from cells (e.g., bacterial cells, animal cells, fungal cells, viruses, yeast cells or plant cells) via lysis and one or more additional isolation procedures, such as one or more filtration and/or chromatography procedures. In particular, the invention relates to compositions, methods and kits wherein protein or peptide molecules are isolated using an integrated lysis/filtration matrix, which may comprise one or more supports (e.g., polyolefin, scintered polyethylene, nitrocellulose, polypropylene, polycarbonate, cellulose acetate, silica, and the like). The compositions, methods and kits of the invention are suitable for isolating a variety of forms of protein or peptide molecules from cells.Type: ApplicationFiled: July 13, 2001Publication date: January 31, 2002Applicant: Invitrogen CorporationInventors: Robert W. Blakesley, Barbara Flynn, Peter Clausen -
Publication number: 20010055792Abstract: Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate are disclosed. More specifically, the present invention concerns inhibiting or preventing pyrophosphorolysis in sequencing and amplification of nucleic acid molecules. According to the present invention, an enzyme which is a pentosyltransferase, a phosphotransferase with an alcohol group as acceptor, a nucleotidyltransferase, or a carboxy-lyase is added to the reaction which serves to remove pyrophosphate from the reaction mixture.Type: ApplicationFiled: June 19, 2001Publication date: December 27, 2001Applicant: Invitrogen CorporationInventor: Robert W. Blakesley
-
Patent number: 6291164Abstract: Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate are disclosed. More specifically, the present invention concerns inhibiting or preventing pyrophosphorolysis in sequencing and amplification of nucleic acid molecules. According to the present invention, an enzyme which is a pentosyltransferase, a phosphotransferase with an alcohol group as acceptor, a nucleotidyltransferase, or a carboxy-lyase is added to the reaction which serves to remove pyrophosphate from the reaction mixture.Type: GrantFiled: November 17, 1997Date of Patent: September 18, 2001Assignee: Invitrogen CorporationInventor: Robert W. Blakesley
-
Patent number: 4889610Abstract: An apparatus provides for casting a gel and for presenting the gel for electrophoresis. The apparatus comprises a casting tray and the casting plate which combine to form a mold for the gelable liquid, and an electrophoresis tank in which the electrophoresis process occurs. The floor of the electrophoresis tank rises to form the plate pedestal upon which the casting plate rests during electrophoresis. The floor of the electrophoresis tank forms a cavity at the edges of the plate pedestal in which the casting tray rests during the electrophoresis process. The casting plate is first firmly seated in the bottom of the casting tray forming a mold for the gelable liquid. The gelable liquid is poured into the mold. The comb is placed in the comb slots so as to form wells in the gel. The gelable liquid is allowed to gel, and the comb is removed. The material to undergo electrophoresis is placed in the wells left by the comb. The entire assembly is then placed and aligned on the plate pedestal.Type: GrantFiled: July 15, 1988Date of Patent: December 26, 1989Assignee: Life Technologies, Inc.Inventors: Robert W. Flesher, Mark S. Berninger, Robert W. Blakesley
-
Patent number: 4773984Abstract: A vertical gel slab electrophoresis apparatus is disclosed comprised of a vertically oriented gel slab arranged between upper and lower buffer solution reservoirs. A bifurcated movable lower tray contains both an upper buffer reservoir drain and lower buffer reservoir. Both upper and lower buffer reservoirs are provided with hinged covers which act as electrical interlocks to prevent unsafe operation of the apparatus, as well as prevent spillage of buffer solution. The design overcomes safety problems associated with both electrical shock and radioactive chemical contamination. The apparatus is provided with handles for each relocation. Cooling vents are provided for ambient air cooling of the apparatus. A gel mold experiment can be assembled and disassembled with unusual ease with the aid of unique self-locking clamp assemblies.Type: GrantFiled: February 2, 1987Date of Patent: September 27, 1988Assignee: Life Technologies, Inc.Inventors: Robert W. Flesher, Kevin J. Barnes, Robert W. Lynn, Robert W. Blakesley
-
Patent number: D303007Type: GrantFiled: February 2, 1987Date of Patent: August 22, 1989Assignee: Life Technologies, Inc.Inventors: Robert W. Flesher, Kevin J. Barnes, Robert W. Blakesley, Robert W. Lynn
-
Patent number: D303012Type: GrantFiled: February 2, 1987Date of Patent: August 22, 1989Assignee: Life Technologies, Inc.Inventors: Robert W. Flesher, Kevin T. Barnes, Robert W. Blakesley, Robert W. Lynn