Patents by Inventor Ronald Flannagan

Ronald Flannagan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20080108127
    Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
    Type: Application
    Filed: December 5, 2007
    Publication date: May 8, 2008
    Applicants: Pioneer Hi-Bred International, Inc., E.I. du PONT de NEMOURS and COMPANY
    Inventors: Andre Abad, Ronald Flannagan, Rafael Hermann, Albert Lu, Billy McCutchen, James Presnail, Janet Rice, James Wong, Cao-Guo Yu
  • Publication number: 20080016596
    Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
    Type: Application
    Filed: September 12, 2007
    Publication date: January 17, 2008
    Inventors: Andre Abad, Ronald Flannagan, Billy McCutchen, Cao Yu
  • Publication number: 20070243536
    Abstract: Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.
    Type: Application
    Filed: April 14, 2006
    Publication date: October 18, 2007
    Applicants: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
    Inventors: Andre Abad, Ronald Flannagan, Bin Hu, Billy McCutchen, Xiaofeng Yang, Cao Yu
  • Publication number: 20070245428
    Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
    Type: Application
    Filed: April 14, 2006
    Publication date: October 18, 2007
    Inventors: Andre Abad, Ronald Flannagan, Billy McCutchen, Cao Yu
  • Publication number: 20070245427
    Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
    Type: Application
    Filed: April 14, 2006
    Publication date: October 18, 2007
    Inventors: Andre Abad, Ronald Flannagan, Billy McCutchen, Cao Yu
  • Publication number: 20060288448
    Abstract: Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided.
    Type: Application
    Filed: June 7, 2006
    Publication date: December 21, 2006
    Applicants: Pioneer Hi-Bred International, Inc., E.I du PONT de NEMOURS and COMPANY
    Inventors: Andre Abad, Gary Chun, Ronald Flannagan, Rafael Herrmann, Albert Lu, Janet Rice, Eric Schepers
  • Publication number: 20060241043
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: June 21, 2006
    Publication date: October 26, 2006
    Applicants: E.I.du PONT de NEMOURS and COMPANY
    Inventors: Ronald Flannagan, Andre Abad
  • Publication number: 20060241044
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: June 21, 2006
    Publication date: October 26, 2006
    Applicants: E.I. du PONT de NEMOURS and COMPANY
    Inventors: Ronald Flannagan, Andre Abad
  • Publication number: 20060242733
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: June 21, 2006
    Publication date: October 26, 2006
    Applicants: E.I. du PONT de NEMOURS and COMPANY
    Inventors: Ronald Flannagan, Andre Abad
  • Publication number: 20060236424
    Abstract: The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.
    Type: Application
    Filed: April 5, 2006
    Publication date: October 19, 2006
    Inventors: Andre Abad, Ronald Flannagan, Rafael Herrmann, Albert Lu, Billy McCutchen, Carl Simmons
  • Publication number: 20060194314
    Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.
    Type: Application
    Filed: May 8, 2006
    Publication date: August 31, 2006
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Ronald Flannagan, Terry Meyer
  • Publication number: 20050261188
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: April 18, 2005
    Publication date: November 24, 2005
    Applicant: E.I. du PONT de NEMOURS and COMPANY
    Inventors: Andre Abad, Ronald Flannagan, Rafael Herrmann, Theodore Kahn, Albert Lu, Billy McCutchen, James Presnail, James Wong, Cao-Guo Yu
  • Publication number: 20050260675
    Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.
    Type: Application
    Filed: July 29, 2005
    Publication date: November 24, 2005
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Ronald Flannagan, John Mathis, Terry Meyer
  • Publication number: 20050262587
    Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.
    Type: Application
    Filed: July 29, 2005
    Publication date: November 24, 2005
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Ronald Flannagan, John Mathis, Terry Meyer
  • Publication number: 20050261483
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: February 15, 2005
    Publication date: November 24, 2005
    Applicant: E.I. duPont de Nemours and Company
    Inventors: Andre Abad, Ronald Flannagan, Rafael Herrmann, Theodore Kahn, Albert Lu, Billy McCutchen, James Presnail, James Wong, Cao-Guo Yu
  • Publication number: 20050166284
    Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
    Type: Application
    Filed: December 22, 2004
    Publication date: July 28, 2005
    Applicants: Pioneer Hi-Bred International, Inc., E.I. du PONT de NEMOURS and COMPANY
    Inventors: Andre Abad, Ronald Flannagan, Rafael Herrmann, Albert Lu, Billy McCutchen, James Presnail, Janet Rice, James Wong, Cao-Guo Yu
  • Publication number: 20050138685
    Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: December 21, 2004
    Publication date: June 23, 2005
    Applicant: E.I du Pont de Nemours and Company
    Inventors: Ronald Flannagan, Andre Abad