Patents by Inventor Sadanori Sekiya

Sadanori Sekiya has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10510524
    Abstract: After various ions of sample origin have been captured within an ion trap, unnecessary ions other than a target ion having a specific m/z are ejected from the ion trap (S1, S2). Subsequently, an operation for dissociating the target ion within the ion trap by hydrogen radical dissociation (HAD), and an operation for sequentially ejecting the thereby generated product ions by resonance excitation from the low m/z side to a point located immediately before the m/z of the target ion, are repeated multiple times (S3-S7). The ions ejected by resonance excitation are detected with a detector to acquire MS/MS spectrum data, and the data obtained by performing the ejection by resonance excitation multiple times are accumulated to create the final MS/MS spectrum (S5, S8).
    Type: Grant
    Filed: January 18, 2016
    Date of Patent: December 17, 2019
    Assignee: SHIMADZU CORPORATION
    Inventors: Sadanori Sekiya, Hidenori Takahashi
  • Publication number: 20190195887
    Abstract: In a mass spectrum of fragment ions obtained by dissociating peptide-derived ions using the technique of irradiating the ions with hydrogen radicals, either pairs of a-type and c-type ions or those of z-type and z-type ions are characteristically observed. Since the mass difference of those ion pairs is previously known, a pair peak searcher 92 searches for pair peaks having a predetermined mass difference in a mass spectrum created by a mass spectrum creator 91, and adds to the detected pair peaks a piece of information indicating that they are pairs of a-type and c-type ions or those of x-type and z-type ions. When estimating the amino acid sequence of the peptide by a database search, a protein identifier 93 uses the ion-pair information in addition to the m/z value of each peak, whereby the accuracy of the estimation or identification the amino acid sequence can be improved.
    Type: Application
    Filed: December 27, 2017
    Publication date: June 27, 2019
    Applicant: SHIMADZU CORPORATION
    Inventors: Sadanori SEKIYA, Hidenori TAKAHASHI
  • Patent number: 10317412
    Abstract: In a mass spectrum of fragment ions obtained by dissociating peptide-derived ions using the technique of irradiating the ions with hydrogen radicals, either pairs of a-type and c-type ions or those of z-type and z-type ions are characteristically observed. Since the mass difference of those ion pairs is previously known, a pair peak searcher 92 searches for pair peaks having a predetermined mass difference in a mass spectrum created by a mass spectrum creator 91, and adds to the detected pair peaks a piece of information indicating that they are pairs of a-type and c-type ions or those of x-type and z-type ions. When estimating the amino acid sequence of the peptide by a database search, a protein identifier 93 uses the ion-pair information in addition to the m/z value of each peak, whereby the accuracy of the estimation or identification the amino acid sequence can be improved.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: June 11, 2019
    Assignee: SHIMADZU CORPORATION
    Inventors: Sadanori Sekiya, Hidenori Takahashi
  • Publication number: 20190108993
    Abstract: After various ions of sample origin have been captured within an ion trap, unnecessary ions other than a target ion having a specific m/z are ejected from the ion trap (S1, S2). Subsequently, an operation for dissociating the target ion within the ion trap by hydrogen radical dissociation (HAD), and an operation for sequentially ejecting the thereby generated product ions by resonance excitation from the low m/z side to a point located immediately before the m/z of the target ion, are repeated multiple times (S3-S7). The ions ejected by resonance excitation are detected with a detector to acquire MS/MS spectrum data, and the data obtained by performing the ejection by resonance excitation multiple times are accumulated to create the final MS/MS spectrum (S5, S8).
    Type: Application
    Filed: January 18, 2016
    Publication date: April 11, 2019
    Applicant: SHIMADZU CORPORATION
    Inventors: Sadanori SEKIYA, Hidenori TAKAHASHI
  • Publication number: 20190004071
    Abstract: Lipid-derived ions captured within an ion trap are irradiated with hydrogen radicals to induce the reaction of hydrogen extraction (S1, S2). A precursor-ion isolation process is subsequently performed (S3), and the precursor ion is dissociated by low-energy collision-induced dissociation (S4). The thereby generated product ions are subjected to mass spectrometry to create a product-ion spectrum (S5, S6). Since the dissociation achieved by such a procedure does not cause hydrogen rearrangement, a peak pair having a mass difference of +12 Da characteristic of the unsaturated bond site certainly appears on the product-ion spectrum. By searching for this peak pair, the unsaturated bond site can be located (S7, S8). By such a method, a lipid-structure analysis including the determination of the position of the unsaturated bond site in a lipid can be performed in a stable and accurate manner without requiring derivatization or other cumbersome pretreatments.
    Type: Application
    Filed: June 27, 2018
    Publication date: January 3, 2019
    Applicant: SHIMADZU CORPORATION
    Inventors: Hidenori TAKAHASHI, Sadanori SEKIYA
  • Patent number: 9947520
    Abstract: A cloud of ions captured in an ion trap (2) is irradiated with a stream of hydrogen radicals cast through a radical particle introduction hole (26) bored in a ring electrode (21) at a flow rate of 4×1010 [atoms/s]. As a result, a radical induced dissociation which does not rely on the transfer or capture of electrons occurs within the ion trap (2), whereby c/z-type fragment ions are efficiently generated. After the irradiation with the hydrogen radicals, a supplemental collision-induced dissociation process may performed by introducing inert gas into the ion trap (2) and resonantly exciting the ions, in order to further promote the generation of the c/z-type fragment ions. In this manner, according to the present invention, it is possible to achieve radical induced dissociation of singly-charged ions derived from a peptide and use the thereby generated c/z-type fragment ions for mass spectrometry.
    Type: Grant
    Filed: February 17, 2015
    Date of Patent: April 17, 2018
    Assignee: SHIMADZU CORPORATION
    Inventors: Hidenori Takahashi, Kei Kodera, Sadanori Sekiya, Kenichi Taniguchi
  • Publication number: 20160372311
    Abstract: A cloud of ions captured in an ion trap (2) is irradiated with a stream of hydrogen radicals cast through a radical particle introduction hole (26) bored in a ring electrode (21) at a flow rate of 4×1010 [atoms/s]. As a result, a radical induced dissociation which does not rely on the transfer or capture of electrons occurs within the ion trap (2), whereby c/z-type fragment ions are efficiently generated. After the irradiation with the hydrogen radicals, a supplemental collision-induced dissociation process may performed by introducing inert gas into the ion trap (2) and resonantly exciting the ions, in order to further promote the generation of the c/z-type fragment ions. In this manner, according to the present invention, it is possible to achieve radical induced dissociation of singly-charged ions derived from a peptide and use the thereby generated c/z-type fragment ions for mass spectrometry.
    Type: Application
    Filed: February 17, 2015
    Publication date: December 22, 2016
    Applicant: SHIMADZU CORPORATION
    Inventors: Hidenori TAKAHASHI, Kei KODERA, Sadanori SEKIYA, Kenichi TANIGUCHI
  • Patent number: 8975575
    Abstract: A mass spectrometry using helium as cooling gas is performed to obtain a first mass spectrum (S1), and another mass spectrometry using argon, which is heavier than helium, as cooling gas is performed to obtain a second mass spectrum for the same sample (S2). Due to the difference between the two gases in terms of the effect of promoting dissociation of modifications, an ion peak originating from a target compound from which all the modifications have been dissociated will appear with a higher intensity on the second mass spectrum. The peak patterns of the two mass spectra are compared to locate the all-dissociated ion peak while excluding unnecessary peaks (S3). Based on that peak, the assignment of each peak is determined (S4).
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: March 10, 2015
    Assignee: Shimadzu Corporation
    Inventors: Sadanori Sekiya, Masaki Murase, Hidenori Takahashi, Kentaro Morimoto
  • Publication number: 20140224973
    Abstract: A mass spectrometry using helium as cooling gas is performed to obtain a first mass spectrum (S1), and another mass spectrometry using argon, which is heavier than helium, as cooling gas is performed to obtain a second mass spectrum for the same sample (S2). Due to the difference between the two gases in terms of the effect of promoting dissociation of modifications, an ion peak originating from a target compound from which all the modifications have been dissociated will appear with a higher intensity on the second mass spectrum. The peak patterns of the two mass spectra are compared to locate the all-dissociated ion peak while excluding unnecessary peaks (S3). Based on that peak, the assignment of each peak is determined (S4).
    Type: Application
    Filed: April 4, 2012
    Publication date: August 14, 2014
    Applicant: SHIMADZU CORPORATION
    Inventors: Sadanori Sekiya, Masaki Murase, Hidenori Takahashi, Kentaro Morimoto
  • Publication number: 20120119083
    Abstract: Ions supplied in the form of a pulse are introduced into an ion trap through an ion entering orifice while a rectangular voltage of a frequency higher than the frequency at which the best trap is accomplished is applied to a ring electrode from a trap voltage generating unit. With this, since a well of a pseudo ion potential is formed in a radial direction in the ion trap, the spread of ions of low m/z values introduced previously is suppressed. A part of ions is introduced into the ion trap, and thereafter the frequency of the rectangular voltage applied to the ring electrode is lowered stepwise to the frequency at which the best trap is accomplished. As a result, the ions of the low m/z values introduced previously can be efficiently trapped, and introduction of ions of high m/z values reaching the ion trap later is not hindered.
    Type: Application
    Filed: March 30, 2009
    Publication date: May 17, 2012
    Applicant: SHIMADZU CORPORATION
    Inventors: Kei Kodera, Makoto Hazama, Shinichi Iwamoto, Sadanori Sekiya
  • Patent number: 8173961
    Abstract: While applying a square wave voltage to the ion electrode (21) so that ions already captured in the ion trap (20) do not disperse, the timing of irradiating a laser light for ion generation is controlled in such a manner that ions reach the ion inlet (25) at a predetermined timing of a cycle of the voltage. In the case of a positive ion (cation) for example, the timing of laser light irradiation is adjusted in such a manner that the target ions reach the ion inlet (25) in the low level period of a cycle of the square wave voltage. By injecting ions in addition to the ions already captured in the ion trap (20) in this manner, the amount of ions can be increased, and by performing a mass separation and detection after that, the signal intensity in one mass analysis can be increased. Accordingly, by decreasing the number of repetitions of the mass analysis for summing up mass profiles, the measuring time can be shortened.
    Type: Grant
    Filed: March 28, 2008
    Date of Patent: May 8, 2012
    Assignee: Shimadzu Corporation
    Inventors: Shinichi Iwamoto, Kei Kodera, Sadanori Sekiya
  • Patent number: 8022363
    Abstract: While applying a square wave voltage to the ion electrode (21) so that ions already captured in the ion trap (20) do not disperse, the frequency of the square wave voltage is temporarily increased at the timing when the ions generated in response to the short time irradiation of a laser light reach the ion inlet (25). This decreases the Mathieu parameter qz, and the potential well becomes shallow, which makes it easy for ions to enter the ion trap (20). Although the ions that have been already captured become more likely to disperse, the frequency of the square wave voltage is decreased before they deviate from the stable orbit. Thus, the dispersion of the ions can also be avoided. Accordingly, while the number of captured ions is not decreased, new ions are further added, and thereby the amount of ions can be increased. By performing a mass separation and detection after that, the signal intensity in one mass analysis can be increased.
    Type: Grant
    Filed: March 28, 2008
    Date of Patent: September 20, 2011
    Assignee: Shimadzu Corporation
    Inventors: Shinichi Iwamoto, Kei Kodera, Sadanori Sekiya
  • Publication number: 20100116982
    Abstract: While applying a square wave voltage to the ion electrode (21) so that ions already captured in the ion trap (20) do not disperse, the timing of irradiating a laser light for ion generation is controlled in such a manner that ions reach the ion inlet (25) at a predetermined timing of a cycle of the voltage. In the case of a positive ion (cation) for example, the timing of laser light irradiation is adjusted in such a manner that the target ions reach the ion inlet (25) in the low level period of a cycle of the square wave voltage. By injecting ions in addition to the ions already captured in the ion trap (20) in this manner, the amount of ions can be increased, and by performing a mass separation and detection after that, the signal intensity in one mass analysis can be increased. Accordingly, by decreasing the number of repetitions of the mass analysis for summing up mass profiles, the measuring time can be shortened.
    Type: Application
    Filed: March 28, 2008
    Publication date: May 13, 2010
    Applicant: Shimadzu Corporation
    Inventors: Shinichi Iwamoto, Kei Kodera, Sadanori Sekiya
  • Publication number: 20050089951
    Abstract: The present invention provides a method for improving the sensitivity of mass spectrometry for biological molecules. The present invention also provides a method for a rapid and simple analysis of biological samples by making use of the method for improving the sensitivity of mass spectrometry. A method for labeled amidation, comprising the step of reacting an amidating reagent labeled with 15N, an isotope of nitrogen, with the carboxyl group of a biological molecule. A method for analyzing a biological molecule, comprising the steps of performing amidation to a carboxyl group of a biological molecule to obtain an amidated form of the biological molecule, and subsequently subjecting the amidated form of the biological molecule to mass spectrometry. A labeled amidated form of a biological molecule wherein a carboxyl group of the biological molecule is amidated as 15N-labeled amide group.
    Type: Application
    Filed: October 22, 2004
    Publication date: April 28, 2005
    Inventors: Koichi Tanaka, Sadanori Sekiya, Yoshinao Wada