Abstract: Introduced here are diagnostic platforms able to optimize computer-aided diagnostic (CADx) models by simulating the optical performance of an imaging device based on its mechanical components. For example, a diagnostic platform may acquire a source image associated with a confirmed diagnosis of a medical condition, simulate optical performance based on design data corresponding to a virtual prototype of the imaging device, generate a training image by altering the source image based on the optical performance, apply a diagnostic model to the training image, and then determine whether the performance of the diagnostic model meets a specified performance threshold. If the diagnostic model fails to meet the specified performance threshold, the diagnostic platform can automatically optimize the diagnostic model for the imaging device by altering its underlying algorithm(s).

Abstract: A retinal imaging system includes an image sensor for acquiring a retinal image and an illuminator for illuminating a retina to acquire the retinal image. The illuminator surrounds an aperture through which an image path for the retinal image passes before reaching the image sensor. Illumination sources surround the aperture.

Abstract: An ophthalmic camera system includes an eyepiece lens disposed in or on a housing. A flexible eyecup has a proximal opening end attached to the housing and surrounding the eyepiece lens and a distal opening end shaped to press against a face around an eye. An image sensor is adapted to acquire a retinal image of the eye through the eyepiece lens when the flexible eyecup is pressed against the face. A field source generates a field. A field sensing system senses the field. One of the field source or the field sensing system is disposed in or on the flexible eyecup and the other one is rigidly mounted to the housing. A controller is coupled to the field sensing system for tracking a relative position of the eyepiece lens relative to the field source to aid aligning the eyepiece lens to the eye.

Abstract: A system for imaging a retina includes an optical relay system having a first lens for positioning proximate to an eye, a light source positioned to illuminate the eye, an image sensor positioned to capture a retinal image of the retina through the first lens, an aperture disposed between the image sensor and the first lens along an optical path extending from the image sensor through the first lens, and a processing apparatus. The aperture surrounds the optical path and is adapted to block off-axis reflections from the eye.

Abstract: A method of determining a number of a solution constituent includes introducing a first number of solution constituents to a first test location, establishing a first binding environment for the introduced first number of solution constituents, creating a first residual number of solution constituents by binding a first plurality of solution constituents, establishing a second binding environment for the first residual number of solution constituents, creating a second residual number of solution constituents by binding a second plurality of solution constituents from the first residual number of solution constituents, obtaining a first signal associated with the bound first plurality of solution constituents, obtaining a second signal associated with the bound second plurality of solution constituents, and determining a second number of a constituent of interest based upon the obtained first signal and the obtained second signal.

Abstract: A bifunctional polymer is functionalized at one end with an azlactone end group to conjugate biomolecules of interest, and is functionalized at another end with an azide anchor group to attach the polymer to a substrate. Methods of making the bifunctional polymer are also provided. A coated substrate includes the bifunctionalized polymers on the surface of a substrate. Methods of making the coated substrate are also provided. A microarray includes a plurality of discrete regions, each region including the coated substrate.

Abstract: A retinal imaging system includes an image sensor for acquiring a retinal image and a dynamic illuminator for illuminating a retina to acquire the retinal image. The dynamic illuminator includes a center baffle along with first and second illumination arrays. The center baffle extends from and surrounds an aperture through which an image path for the retinal image passes before reaching the image sensor. The first illumination array extends out from first opposing sides of the aperture along a first linear axis. The second illumination array extends out from second opposing sides of the aperture along a second linear axis that is substantially orthogonal to the first linear axis.

Abstract: The technology described herein is directed to a fundus camera and, more specifically, to a fundus camera having a display that projects active visual alignment stimuli onto an eye of an examinee via one or more components of an optimal assembly. The active visual alignment stimuli are dynamically adjusted to guide an examinee toward optical alignment for fundus imaging.

Abstract: A method of imaging an interior of an eye includes illuminating the interior of the eye with one or more beams of light from a light source, and said illuminating is configured to trigger a change to a pupil of the eye. An image sensor captures a sequence of images of light reflected by the interior of the eye during an expected timeframe when a width of the pupil is changing in response to said illuminating. A processing apparatus combines images in the sequence of images to form a composite image having a larger depth of field than a depth of field of each of the images in the sequence of images. The depth of field for each of the images corresponds to the width of the pupil when each of the images is captured.

Abstract: A system and method of quantitating the concentration of a molecule of interest in one embodiment includes establishing a plurality of test environments at a plurality of test sites, each of the plurality of test environments associated with one of a plurality of response curves, each of the plurality of response curves different from the other of the plurality of response curves, storing a combined response curve resulting from a summation of the plurality of response curves, exposing the plurality of test sites to a sample having a concentration of a molecule of interest, obtaining a plurality of quantitation signals, each of the plurality of quantitation signals associated with one of the plurality of test sites, associating a summation of the plurality of quantitation signals with the stored combined response curve, and generating a signal related to the concentration of the molecule of interest based upon the association.

Abstract: An apparatus for imaging an interior of an eye includes a light sensitive sensor, a plurality of light emitters (LEs) capable of outputting light, a plurality of nonvisible light emitters (NV-LEs) capable of outputting nonvisible light, and a controller. The controller is coupled to the plurality of LEs, the plurality of NV-LEs, and the light sensitive sensor, and the controller implements logic that when executed by the controller causes the apparatus to perform operations. The operations include illuminating the eye with the nonvisible light from the plurality of NV-LEs, and determining an amount of reflection of the nonvisible light from the eye for each of the NV-LEs in the plurality of NV-LEs. The operations also include illuminating the eye with selected one or more of the LEs in the plurality of LEs, and capturing, with the light sensitive sensor, a sequence of images of the interior of the eye while the eye is illuminated with the light from the LEs.

Abstract: The technology described herein is directed to a fundus camera and, more specifically, to a fundus camera having a display that projects active visual alignment stimuli onto an eye of an examinee via one or more components of an optimal assembly. The active visual alignment stimuli are dynamically adjusted to guide an examinee toward optical alignment for fundus imaging.

Abstract: A dynamic illuminator includes a central aperture, a ring of inner light sources, a plurality of primary illumination arrays, and a plurality of secondary illumination arrays. The ring of inner light sources is arranged around the central aperture. The plurality of primary illumination arrays extends along radial axes from the central aperture outside of the ring of inner light sources, wherein the primary illumination arrays each includes a plurality of primary light sources. The plurality of secondary illumination arrays is disposed along secondary axes extending from the central aperture outside of the ring of inner light sources. The secondary illumination arrays each includes a plurality of secondary light sources. The secondary axes of the secondary illumination arrays are disposed angularly between adjacent ones of the primary illumination arrays.

Abstract: Device and methods for use in a biosensor comprising a multisite array of test sites, the device and methods being useful for modulating the binding interactions between a (biomolecular) probe or detection agent and an analyte of interest by modulating the pH or ionic gradient near the electrodes in such biosensor. An electrochemically active agent that is suitable for use in biological buffers for changing the pH of the biological buffers. Method for changing the pH of biological buffers using the electrochemically active agents. The methods of modulating the binding interactions provided in a biosensor, analytic methods for more accurately controlling and measuring the pH or ionic gradient near the electrodes in such biosensor, and analytic methods for more accurately measuring an analyte of interest in a biological sample.

Abstract: Disclosed is a headset for monitoring periocular humidity, the headset including a housing configured to be positioned on a user's face and defining a periocular air space. The headset includes a humidity sensor configured to measure a humidity of air within the periocular air space, and an ophthalmic testing unit configured to measure a physiological parameter of an eye of the user. The headset further includes a processor coupled to the humidity sensor and the ophthalmic testing unit. The processor is configured to receive the measured humidity and the measured physiological parameter, and determine an ophthalmic condition based on the measured humidity and the measured physiological parameter.

Abstract: Device and methods for controlling pH or ionic gradient comprising a multisite array of feedback electrode sets comprising electrodes and pH sensing elements. The electrodes can include a reference electrode, counter electrode, and a working electrode. The device and methods iteratively select an amount of current and/or voltage to be applied to each working electrode, apply the selected amount of current and/or voltage to each working electrode to change pH of a solution close to the working electrode, and measure the signal output of the sensing element. The multisite array can include feedback and non-feedback electrode sets.

Abstract: A technique for retinal images includes configuring a dynamic illuminator to illuminate the retina with an illumination pattern. An image frame of the retina is captured while illuminated with the illumination pattern. The configuring and the capturing are iterated to sequentially reconfigure the dynamic illuminator to illuminate the retina with a plurality of different illumination patterns and to capture a plurality of image frames each illuminated with one of the different illumination patterns. The image frames are combined into a composite retinal image.

Abstract: A vision traffic marker network system includes a management system and a plurality of self-powered wireless traffic nodes configured to collect data from a wireless mesh network. Each wireless traffic node is configured to function as a mesh network traffic node and in each mesh network traffic node, the mesh network traffic node is configured to exchange data between the traffic nodes. Each wireless traffic node in the vision traffic marker system is communicatively coupled to any server and client machine. The management system includes a data acquisition layer, a processing layer, a communication layer, and a management layer. The acquisition layer is configured to receive data from the plurality of wireless traffic nodes and transmit the acquired data to the processing layer so that the acquired data is processed and filtered before the data is broadcasted and distributed the plurality of wireless traffic nodes which in turn exchange or share the data with any server and client machine.

Abstract: Introduced here are retinal cameras having optical stops whose size and/or position can be modified to increase the size of the space in which an eye can move while being imaged. In some embodiments, an optical stop is mechanically moved to recover retinal image quality as the subject shifts their eye. In some embodiments, an optical stop is digitally created using a pixelated liquid crystal display (LCD) layer having multiple pixels that are individually controllably. In some embodiments, multiple non-pixelated LCD layers are connected to one another to form a variable transmission stack, and each LCD layer within the variable transmission stack may be offset from the other LCD layers. In such embodiments, the optical stop can be moved by changing which LCD layer is active at a given point in time.

Abstract: A technique for performing a visual field test on an eye includes sequentially presenting visual stimuli to the eye on a display. A gaze direction of the eye is monitored with a camera to identify when the gaze direction has drifted from a fixation target. A first intended position associated with a first visual stimulus of the visual stimuli is offset to compensate for drifting of the gaze direction from the fixation target when the gaze direction is determined to have drifted during presentation of the first visual stimulus. User inputs indicating whether the user acknowledges observance of each of the visual stimuli presented are registered.