Abstract: A method for detecting HER2-positive cancer cells, comprising steps of: (a) fixing and then permeabilizing cells; (b) contacting a primary antibody that recognizes an intracellular epitope of HER2 with the cells and then contacting a secondary antibody that recognizes the primary antibody and is labeled with a fluorescent dye; and (c) irradiating the cells with excitation light for the fluorescent dye and detecting fluorescence emitted from the cells is provided. According to such a method, HER2-positive cancer cells can be detected with high sensitivity.

Abstract: The present invention provides a method for detecting circulating tumor cells, comprising steps of: capturing cells in a blood sample on a filter; contacting an antibody that is labeled with a first fluorescent dye and recognizes a leukocyte marker protein with the cell-captured filter; contacting an animal serum, a surfactant and an antibody that is labeled with a second fluorescent dye and recognizes an epithelial cell marker protein with the cell-captured filter; contacting a third fluorescent dye that stains nucleic acids with the cell-captured filter; and detecting fluorescences emitted from each cell captured on the filter.

Abstract: The present invention relates to a standard cell suspension for confirming the stainability of a treatment solution that differentially stains circulating tumor cells (CTCs) and leucocytes, containing (a) a cell line having an antigen that is specific for human cancer cells and (b) a cell line having an antigen that is specific for human leukocytes, wherein the (a) and (b) components are each a cell line that is fixed by a cell-fixing reagent.

Abstract: A biomolecule capturing filter, comprising a gold plating on the surface of a biomolecule capturing filter made of a metal other than gold, the gold plating being electroless gold plating is disclosed.

Abstract: Provided is a cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid, and the method includes a step of draining a liquid for n (n is a natural number) times, in which from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.

Abstract: A method for capturing rare cells in blood allows for the preparation of a sample containing the capture of rare cells in blood suitable for a downstream. A method for capturing rare cells in blood includes isolating rare cells in blood from blood, the method including a step of removing white blood cells from the blood after blood filtration using a filter.

Abstract: A method for capturing rare cells in blood allows for the preparation of a sample containing the capture of rare cells in blood suitable for a downstream. A method for capturing rare cells in blood includes isolating rare cells in blood from blood, the method including a step of removing white blood cells from the blood before blood filtration using a filter.

Abstract: A polymer comprising a constitutional unit represented by the following formula (1) is useful because when the polymer is applied to a surface of a cancer cell enrichment filter, adhesiveness of cancer cells to the filter surface can be improved so that an enrichment ratio of the cancer cells can be improved. In the formula (1), R1 is a hydrogen atom or a methyl group, R2 is a methyl group or an ethyl group and m is 1 to 3.

Abstract: A cell-trapping system according to one embodiment of the present invention is a cell-trapping system which traps specific cells in blood by passing the blood from a first principal surface side of a filter with a plurality of through-holes formed across a thickness of a sheet toward a second principal surface side opposed to the first principal surface, wherein a linear velocity of the blood at a point in time when the blood passes through the filter is 1 cm/min to 40 cm/min, an aperture ratio of the filter is 3% to 10%, the plurality of through-holes disposed in the filter each have a rectangular shape or a rounded-corner rectangular shape, and a mean of minor pore diameters of the plurality of through-holes on the first principal surface side is 7.0 ?m to 10.0 ?m.

Abstract: The methods of rare cells collection from enclosed micro filter system are provided. This invention includes surface treatment of the filter, application of cells dissociation reagent, shaking/vibration and back/side flush systems.

Abstract: A biomolecule capturing filter, comprising a gold plating on the surface of a biomolecule capturing filter made of a metal other than gold, the gold plating being electroless gold plating is disclosed.

Abstract: A polymer comprising a constitutional unit represented by the following formula (1) is useful because when the polymer is applied to a surface of a cancer cell enrichment filter, adhesiveness of cancer cells to the filter surface can be improved so that an enrichment ratio of the cancer cells can be improved. In the formula (1), R1 is a hydrogen atom or a methyl group, R2 is a methyl group or an ethyl group and m is 1 to 3.