Abstract: The present invention provides a prosthetic tissue or sheet capable of withstanding implantation operations, which can be used in actual operation and can be produced by culture. The present invention also provides a novel therapy which can substitute for cell therapy. Particularly, the present invention provides a method for producing a prosthetic tissue comprising a cell derived from a part other than myocardium and capable of withstanding implantation operation. The above-described objects of the present invention were partially achieved by finding that by culturing cells under specific culture conditions, the cells are unexpectedly organized into a tissue, and the resultant prosthetic tissue is capable of being detached from culture dishes. The present invention also provides a three-dimensional structure applicable to heart, comprising a cell derived from a part other than the myocardium of an adult.

Abstract: The present invention provides a prosthetic tissue or sheet capable of withstanding implantation operations, which can be used in actual operation and can be produced by culture. The present invention also provides a novel therapy which can substitute for cell therapy. Particularly, the present invention provides a method for producing a prosthetic tissue comprising a cell derived from a part other than myocardium and capable of withstanding implantation operation. The above-described objects of the present invention were partially achieved by finding that by culturing cells under specific culture conditions, the cells are unexpectedly organized into a tissue, and the resultant prosthetic tissue is capable of being detached from culture dishes. The present invention also provides a three-dimensional structure applicable to heart, comprising a cell derived from a part other than the myocardium of an adult.

Abstract: Decellularization of tissue by means of an amphipathic solvent a well-established practice. However, situations exist where the provision of enhanced decellularization is preferred. There is a demand for treating methods for coping with such situations. Thus, it is intended to provide a method for enhancing decellularization. The method comprises not only the immersing of a tissue in a solution containing an amphiphilic molecule in non-micellar form (for example, 1,2-epoxide polymer) but also performing a radical reaction (for example, treatment selected from the group consisting of exposure to gamma-ray irradiation, ultraviolet irradiation, a free radical supply source, ultrasonication, electron beam irradiation, and X-ray irradiation).

Abstract: The invention provides a reagent-introducing medical device that can guide a reagent into a reagent injector while maintaining all biological materials contained in the reagent in a healthy state. In an embodiment of the reagent-introducing medical device, an apparatus body (12) comprises a channel (77, 78, 80, 50e) through which a reagent flows, as well as a reagent inlet (76) and outlet (21), and further comprises a first control mechanism (58, 64) for controlling the flow of the reagent to create a laminar reagent flow in the channel (77, 78, 80, 50e) and a second control mechanism (70) for further controlling the laminar reagent flow in the channel to create a rotational reagent flow.

Abstract: The present invention provides a prosthetic tissue or sheet capable of withstanding implantation operations, which can be used in actual operation and can be produced by culture. The present invention also provides a novel therapy which can substitute for cell therapy. Particularly, the present invention provides a method for producing a prosthetic tissue comprising a cell derived from a part other than myocardium and capable of withstanding implantation operation. The above-described objects of the present invention were partially achieved by finding that by culturing cells under specific culture conditions, the cells are unexpectedly organized into a tissue, and the resultant prosthetic tissue is capable of being detached from culture dishes. The present invention also provides a three-dimensional structure applicable to heart, comprising a cell derived from a part other than the myocardium of an adult.

Abstract: It is a purpose to provide a medicinal-liquid injection apparatus in which a needle-like tubular member is smoothly movable in a lumen without damaging an inner circumferential surface of the lumen. It includes that the needle-like tubular member having an acuminate needle portion as a free end portion thereof, in which a medicinal liquid can flow, is inserted in the lumen of a tubular main body insertable in a body of a living being, and a protection member having a flexible portion provided in a state such that the protection member is movable together with the needle-like tubular member and such that the flexible portion is interposed between an inner circumferential surface of the lumen and a tip of the needle portion of the needle-like tubular member.

Abstract: A surgical holder comprises a grasping member for grasping a tissue, a manipulation member for manipulating the grasping member, and a connection portion with one end connected to the manipulation member, wherein: the grasping member includes a first grasping plate, and a second grasping plate provided so as to oppose the first grasping plate in a movable manner so that they are able to become closer to each other or more distranced from each other; a first grasping portion which can grasp a part of a tissue between the first grasping plate and the second grasping plate, and an opening which can expose another part of the tissue are provided in one end portion of the grasping member; and a second grasping portion which can form a tissue grasping space between the first grasping plate and the second grasping plate is provided in another end portion of the grasping member.

Abstract: The present invention provides an implant capable of being cellularized in treatment of an injured organ or tissue in organisms. The present inventors found that a biocompatible implant comprising a biological molecule and a support is capable of being cellularized. The implant can be used instead of conventional implants which essentially comprise cells. The present invention provides a biocompatible implant comprising A) a biological molecule and B) a support. The present invention also provides A) a first layer having a rough surface, B) a rough surface; B) a second layer having a strength which allows the support to resist in vivo shock. The first layer is attached to the second layer via at least one point.

Abstract: It is a purpose to provide a medicinal-liquid injection apparatus in which a needle-like tubular member is smoothly movable in a lumen without damaging an inner circumferential surface of the lumen. It includes that the needle-like tubular member having an acuminate needle portion as a free end portion thereof, in which a medicinal liquid can flow, is inserted in the lumen of a tubular main body insertable in a body of a living being, and a protection member having a flexible portion provided in a state such that the protection member is movable together with the needle-like tubular member and such that the flexible portion is interposed between an inner circumferential surface of the lumen and a tip of the needle portion of the needle-like tubular member.

Abstract: A method for injecting a liquid drug containing a biological material is provided, which comprises the step of injecting the liquid drug containing the biological material contained in an injector into a subject at a predetermined range of velocity.

Abstract: An objective of the present invention is to overcome a problem that there is an inverse relationship between the decellularization rate and the strength of tissue. This problem was solved by immersing tissue in a solution containing a non-micellar amphipathic molecule (e.g., a 1,2-epoxide polymer). Thus, the present invention provides decellularized tissue, in which the cell survival rate of the tissue is less than a level at which calcification or an immune reaction is elicited in an organism and the tissue damage rate of the tissue is suppressed to a level which permits clinical applications. Tissue prepared by the above-described treatment preferably retains a certain level of tissue strength. Further, the tissue of the present invention has an effect of performing cell replacement.

Abstract: A method for injecting a liquid drug containing a biological material is provided, which comprises the step of injecting the liquid drug containing the biological material contained in an injector into a subject at a predetermined range of velocity.