Abstract: An optical element comprises a planoconvex basic shape along an optical axis of the optical element; a third portion arranged between a first portion and a second portion. The first portion includes a plane side face for facing an object to be imaged. The second portion includes a convexly shaped side face for facing an image plane. The plane side face of the first portion is designed to collect and steer a radiation to be captured into the optical element, the optical element being designed to guide rays of the captured radiation in a beam path. The first, the second and the third portions are arranged directly adjacent to one another. A refractive index of the third portion is greater than a refractive index of the second portion, and the refractive index of the second portion is greater than a refractive index of the first portion.

Abstract: An arrangement and method for supplying immersion media and a method for setting optical parameters of a medium, includes a media supply unit for the controlled supply of a medium or of a mixture into a contact region between an optical lens and a specimen slide, on which a specimen may be arranged. An image capture unit is provided for capturing image data on the basis of detection radiation from the object space along a detection beam path extending through the contact region. An evaluation unit is provided to establish current image parameters on the basis of captured image data, to compare said current image parameters to intended image parameters and to establish a desired mixing ratio of at least two components of the medium depending on the comparison.

Abstract: An arrangement and method for supplying immersion media and a method for setting optical parameters of a medium, includes a media supply unit for the controlled supply of a medium or of a mixture into a contact region between an optical lens and a specimen slide, on which a specimen may be arranged. An image capture unit is provided for capturing image data on the basis of detection radiation from the object space along a detection beam path extending through the contact region. An evaluation unit is provided to establish current image parameters on the basis of captured image data, to compare said current image parameters to intended image parameters and to establish a desired mixing ratio of at least two components of the medium depending on the comparison.

Abstract: Currently, relatively weak light sources with low light intensities are used in wide-field microscopes. Inevitably, focusing in the pupil plane thereby results in low light intensities in the sample, since the output of the light source is distributed over a very large sample area. However, there are also wide-field technologies requiring very high intensities, for example, photo-activates localization (PAL) microscopy. It is the object of the invention to allow, with little expenditure, the flexible adjustment of the illumination light intensity in the sample. For this purpose, a laser (2) is used for a light source, and a variable lens (10) is arranged in the illumination beam path thus making a variable adjustment of a bean cross section of the illumination light in an intermediate image plane possible, wherein a divergence of the illumination light is identical for different beam cross sections. In this way, the size of the visual field of the microscope can be flexibly adjusted.

Abstract: The invention relates to a microscope, more particularly to a fluorescence microscope, for the structured illumination microscopy, comprising a microscope light path having an optical axis, including a beam splitter for coupling illumination light into the microscope light path, including an illumination pattern unit disposed, in particular, in the microscope light path for the purpose of generating an illuminated pattern on, or in, a sample to be examined, comprising a rotary device for the purpose of effecting relative rotation about the optical axis between the illumination pattern and the sample to be examined.