Patents by Inventor Selena Chan

Selena Chan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7476786
    Abstract: The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by scanning probe microscopy (SPM), such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and scanning probe microscopy (SPM) analysis. Compositions comprising coded probes are also disclosed herein.
    Type: Grant
    Filed: September 19, 2003
    Date of Patent: January 13, 2009
    Assignee: Intel Corporation
    Inventors: Selena Chan, Xing Su, Mineo Yamakawa
  • Patent number: 7442339
    Abstract: Disclosed herein are methods, apparatuses, and systems for performing nucleic acid sequencing reactions and molecular binding reactions in a microfluidic channel. The methods, apparatuses, and systems can include a restriction barrier to restrict movement of a particle to which a nucleic acid is attached. Furthermore, the methods, apparatuses, and systems can include hydrodynamic focusing of a delivery flow. In addition, the methods, apparatuses, and systems can reduce non-specific interaction with a surface of the microfluidic channel by providing a protective flow between the surface and a delivery flow.
    Type: Grant
    Filed: March 31, 2004
    Date of Patent: October 28, 2008
    Assignee: Intel Corporation
    Inventors: Narayanan Sundararajan, Lei Sun, Yuegang Zhang, Xing Su, Selena Chan, Tae-Woong Koo, Andrew A. Berlin
  • Patent number: 7432112
    Abstract: The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.
    Type: Grant
    Filed: May 19, 2005
    Date of Patent: October 7, 2008
    Assignee: Intel Corporation
    Inventors: Tae-Woong Koo, Selena Chan, Xing Su, Jingwu Zhang, Lei Sun
  • Patent number: 7432113
    Abstract: The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.
    Type: Grant
    Filed: May 19, 2005
    Date of Patent: October 7, 2008
    Assignee: Intel Corporation
    Inventors: Tae-Woong Koo, Selena Chan, Xing Su, Jingwu Zhang, Lei Sun
  • Patent number: 7427513
    Abstract: The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.
    Type: Grant
    Filed: May 19, 2005
    Date of Patent: September 23, 2008
    Assignee: Intel Corporation
    Inventors: Tae-Woong Koo, Selena Chan, Xing Su, Jingwu Zhang, Lei Sun
  • Patent number: 7400395
    Abstract: The disclosed methods and apparatus concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates. Porous silicon substrates may be formed by anodic etching in dilute hydrofluoric acid. A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon by cathodic electromigration or any known technique. In certain alternatives, the metal coated porous silicon substrate comprises a plasma-oxidized, dip and decomposed porous silicon substrate. The metal-coated substrate provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain alternatives, metal nanoparticles may be added to the metal-coated substrate to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus.
    Type: Grant
    Filed: October 31, 2005
    Date of Patent: July 15, 2008
    Assignee: Intel Corporation
    Inventors: Selena Chan, Andrew A. Berlin, Sunghoon Kwon, Narayanan Sundararajan, Mineo Yamakawa
  • Publication number: 20080108131
    Abstract: The invention provides methods used to analyze the contents of a biological sample, such as blood serum, with cascade Raman sensing. A fluorescence producing nanoporous biosensor having probes that bind specifically to known analytes is contacted with a biological sample and one or more bound complexes coupled to the porous semiconductor structure are formed. The bound complexes are contacted with a Raman-active probe that binds specifically to the bound complexes and the biosensor is illuminated to generate fluorescent emissions from the biosensor. These fluorescent emissions generate Raman signals from the bound complexes. The Raman signals produced by the bound complexes are detected and the Raman signal associated with a bound protein-containing analyte is indicative of the presence of the protein-containing compound in the sample. The invention methods are useful to provide a protein profile of a patient sample. The invention also provides detection systems useful to practice the invention methods.
    Type: Application
    Filed: February 1, 2007
    Publication date: May 8, 2008
    Applicant: INTEL CORPORATION
    Inventors: Selena Chan, Tae-Woong Koo
  • Patent number: 7361313
    Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.
    Type: Grant
    Filed: February 18, 2003
    Date of Patent: April 22, 2008
    Assignee: Intel Corporation
    Inventors: Selena Chan, Sunghoon Kwon, Narayan Sundararajan
  • Patent number: 7361821
    Abstract: The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein.
    Type: Grant
    Filed: September 20, 2002
    Date of Patent: April 22, 2008
    Assignee: Intel Corporation
    Inventors: Selena Chan, Xing Su, Mineo Yamakawa
  • Patent number: 7351591
    Abstract: The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.
    Type: Grant
    Filed: March 30, 2004
    Date of Patent: April 1, 2008
    Assignee: Intel Corporation
    Inventors: Tae-Woong Koo, Selena Chan, Xing Su, Jingwu Zhang, Lei Sun
  • Patent number: 7330252
    Abstract: Embodiments of the present invention provide a miniaturized spectroscopy system comprising a light source fabricated from porous silicon. Porous silicon light emitting devices can provide tunable, narrow, and directional luminescence. Advantageously, a porous silicon light source can be integrated into a silicon wafer based device thus simplifying the manufacture of a miniaturized spectros copy system and lab-on-a-chip type devices employing spectroscopic detection.
    Type: Grant
    Filed: December 30, 2005
    Date of Patent: February 12, 2008
    Assignee: Intel Corporation
    Inventors: Tae-Woong Koo, Selena Chan
  • Publication number: 20080032297
    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
    Type: Application
    Filed: February 9, 2007
    Publication date: February 7, 2008
    Applicant: INTEL CORPORATION
    Inventors: Xing Su, Andrew Berlin, Selena Chan, Steven Kirch, Tae-Woong Koo, Gabi Neubauer, Valluri Rao, Narayanan Sundararajan, Mineo Yamakawa
  • Patent number: 7291466
    Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.
    Type: Grant
    Filed: April 20, 2005
    Date of Patent: November 6, 2007
    Assignee: Intel Corporation
    Inventors: Xing Su, Selena Chan, Tae-Woong Koo, Mineo Yamakawa, Andrew A. Berlin
  • Patent number: 7279134
    Abstract: Microfluidic devices with porous membranes for molecular sieving, metering, and separation of analyte fluids. In one aspect, a microfluidic device includes a substrate having input and output microfluidic channel sections separated by a porous membrane formed integral to the substrate. In another aspect, the porous membrane may comprise a thin membrane that is sandwiched between upper and lower substrate members. The microfluidic device may include one or a plurality of porous membranes. In one embodiment, a plurality of porous membranes having increasingly smaller pores are disposed along portions of a microfluidic channel. In another embodiment, a cascading series of upper and lower channels are employed, wherein each upper/lower channel interface is separated by a respective porous membrane. In another aspect, a porous membrane is rotatably coupled to a substrate within a microfluidic channel via a MEMS actuator to enable the porous membrane to be positioned in filtering and pass-through positions.
    Type: Grant
    Filed: September 17, 2002
    Date of Patent: October 9, 2007
    Assignee: Intel Corporation
    Inventors: Selena Chan, Narayan Sundararajan, Andrew A. Berlin, Mineo Yamakawa
  • Patent number: 7270952
    Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.
    Type: Grant
    Filed: September 24, 2002
    Date of Patent: September 18, 2007
    Assignee: Intel Corporation
    Inventors: Xing Su, Selena Chan, Tae-Woong Koo, Mineo Yamakawa, Andrew A. Berlin
  • Patent number: 7271896
    Abstract: The invention provides methods used to analyze the contents of a biological sample, such as blood serum, with cascade Raman sensing. A fluorescence producing nanoporous biosensor having probes that bind specifically to known analytes is contacted with a biological sample and one or more bound complexes coupled to the porous semiconductor structure are formed. The bound complexes are contacted with a Raman-active probe that binds specifically to the bound complexes and the biosensor is illuminated to generate fluorescent emissions from the biosensor. These fluorescent emissions generate Raman signals from the bound complexes. The Raman signals produced by the bound complexes are detected and the Raman signal associated with a bound protein-containing analyte is indicative of the presence of the protein-containing compound in the sample. The invention methods are useful to provide a protein profile of a patient sample. The invention also provides detection systems useful to practice the invention methods.
    Type: Grant
    Filed: December 29, 2003
    Date of Patent: September 18, 2007
    Assignee: Intel Corporation
    Inventors: Selena Chan, Tae-Woong Koo
  • Publication number: 20070153266
    Abstract: Embodiments of the present invention provide a miniaturized spectroscopy system comprising a light source fabricated from porous silicon. Porous silicon light emitting devices can provide tunable, narrow, and directional luminescence. Advantageously, a porous silicon light source can be integrated into a silicon wafer based device thus simplifying the manufacture of a miniaturized spectros copy system and lab-on-a-chip type devices employing spectroscopic detection.
    Type: Application
    Filed: December 30, 2005
    Publication date: July 5, 2007
    Inventors: Tae-Woong Koo, Selena Chan
  • Publication number: 20070155022
    Abstract: Embodiments of the present invention provide methods for determining the degenerate binding capabilities of antibodies. The methods provide information about degenerate binding capabilities without the use of involved procedures. Optionally, a molecule toward which an antibody exhibits degenerate binding ability may be identified through the use of a reporter, such as, a composite organic inorganic nanocluster (COIN). COINs are sensitive SERS (surface enhanced Raman spectroscopy) reporters capable of multiplex analysis of analytes.
    Type: Application
    Filed: December 30, 2005
    Publication date: July 5, 2007
    Inventors: Mineo Yamakawa, Narayan Sundararajan, Andrew Berlin, Selena Chan, Xing Su, Tae-Woong Koo, Lei Sun, Kung-Bin Sung, Mark Roth
  • Patent number: 7238477
    Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.
    Type: Grant
    Filed: September 12, 2003
    Date of Patent: July 3, 2007
    Assignee: Intel Corporation
    Inventors: Xing Su, Andrew A. Berlin, Selena Chan, Steven J. Kirch, Tac-Woong Koo, Gabi Neubauer, Valluri Rao, Narayanan Sundararajan, Mineo Yamakawa
  • Publication number: 20070141714
    Abstract: Embodiments of the invention relate to detecting binding of a first analyte to a second analyte by Raman spectroscopy. An embodiment includes attaching one analyte to a substrate and then detecting the binding of another analyte to the analyte on the substrate by Raman spectroscopy. Another embodiment includes contacting analytes in a fluid and then detecting the binding of one analyte to another analyte by Raman spectroscopy.
    Type: Application
    Filed: December 19, 2005
    Publication date: June 21, 2007
    Applicant: Intel Corporation
    Inventors: Kung-bin Sung, Narayan Sundararajan, Selena Chan