Patents by Inventor Sergei Svitashev

Sergei Svitashev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10150956
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: December 11, 2018
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED IINTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young
  • Publication number: 20180327785
    Abstract: Compositions and methods are provided for restoring function to a non-functional gene product in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to restore function to a non-functional gene product and to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. The present disclosure also describes methods for modifying a nucleotide sequence in the genome of a cell using a restored functional selectable marker, as well as methods for editing a nucleotide sequence in the genome a cell without introducing a polynucleotide modification template into said cell. Compositions and methods are also provided for DNA free delivery of Cas endonucleases, sgRNAs and guide RNA/Cas complexes.
    Type: Application
    Filed: July 24, 2018
    Publication date: November 15, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
  • Publication number: 20180305706
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Application
    Filed: June 13, 2018
    Publication date: October 25, 2018
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
  • Publication number: 20180282763
    Abstract: Compositions and methods are provided for restoring function to a non-functional gene product in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to restore function to a non-functional gene product and to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. The present disclosure also describes methods for modifying a nucleotide sequence in the genome of a cell using a restored functional selectable marker, as well as methods for editing a nucleotide sequence in the genome a cell without introducing a polynucleotide modification template into said cell. Compositions and methods are also provided for DNA free delivery of Cas endonucleases, sgRNAs and guide RNA/Cas complexes.
    Type: Application
    Filed: October 17, 2016
    Publication date: October 4, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
  • Publication number: 20180273960
    Abstract: Compositions and methods are provided for modifying a nucleotide sequence in the genome of a plant cell, without the use of a selectable marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to make a double strand break in a target site located in a nucleotide sequence and plant cells are obtained without the use of a selectable marker, and to provide an effective system for modifying target sites within the genome of a plant, plant cell or seed. Compositions and methods are also provided for producing a plant cell, callus tissue or plant having a modified nucleotide sequence in its genome, without the use of a selectable marker.
    Type: Application
    Filed: October 17, 2016
    Publication date: September 27, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
  • Patent number: 10030245
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: July 24, 2018
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Michael Lassner, Derek Jantz, James Jefferson Smith, Mark Cigan, Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev
  • Publication number: 20180142223
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Application
    Filed: January 10, 2018
    Publication date: May 24, 2018
    Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ERICKA BERMUDEZ, ANDREW MARK CIGAN, JAMES J ENGLISH, SAVERIO CARL FALCO, HUIRONG GAO, LU LIU, ZHAN-BIN LIU, AZALEA S ONG, SERGEI SVITASHEV, JOSHUA K YOUNG
  • Patent number: 9909110
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Grant
    Filed: October 11, 2016
    Date of Patent: March 6, 2018
    Assignees: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Andrew Mark Cigan, James English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea Ong, Sergei Svitashev, Joshua K Young
  • Publication number: 20180002715
    Abstract: Compositions and methods are provided for regulated expression of a guide RNA/Cas endonuclease system in a plant cell, plant and seed. Compositions and methods are also provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a regulated guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed.
    Type: Application
    Filed: February 15, 2016
    Publication date: January 4, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
  • Publication number: 20170022488
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Application
    Filed: October 11, 2016
    Publication date: January 26, 2017
    Inventors: ERICKA BERMUDEZ, ANDREW MARK CIGAN, JAMES ENGLISH, CARL FALCO, HUIRONG GAO, LU LIU, ZHAN-BIN LIU, AZALEA ONG, SERGEI SVITASHEV, JOSHUA K YOUNG
  • Patent number: 9499827
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Grant
    Filed: May 3, 2013
    Date of Patent: November 22, 2016
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Andrew Mark Cigan, James English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea Ong, Sergei Svitashev, Joshua K Young
  • Publication number: 20160208272
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: August 20, 2014
    Publication date: July 21, 2016
    Inventors: Andrew Cigan, Saverio Carl Falco, Huirong GAO, Zhongsen Li, Zhan-Bin Liu, L. Aleksander Lyznik, Jinrui Shi, Sergei Svitashev, Joshua K. Young
  • Publication number: 20160208271
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.
    Type: Application
    Filed: August 20, 2014
    Publication date: July 21, 2016
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Michael Lassner, Zhan-Bin Liu, Sergei Svitashev
  • Publication number: 20150184173
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Application
    Filed: May 1, 2013
    Publication date: July 2, 2015
    Inventors: Ericka Bermudez, Andrew Mark Cigan, James English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-bin Liu, Azalea Ong, Sergei Svitashev, Joshua K. Young
  • Publication number: 20150082478
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: August 20, 2014
    Publication date: March 19, 2015
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, L. Aleksander Lyznik, Jinrui Shi, Sergei Svitashev, Joshua K. Young
  • Publication number: 20130263324
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Application
    Filed: March 22, 2012
    Publication date: October 3, 2013
    Applicants: E.I. du Pont de Nemours and Company, Pioneer Hi-Bred International, Inc.
    Inventors: Michael Lassner, Derek Jantz, James Jefferson Smith, Mark Cigan, Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev
  • Publication number: 20110119795
    Abstract: Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
    Type: Application
    Filed: September 30, 2010
    Publication date: May 19, 2011
    Inventors: EVGUENI ANANIEV, Olga N. Danilevskaya, MARK A. CHAMBERLIN, WILLIAM J. GORDON-KAMM, SERGEI SVITASHEV, CHENGCANG WU
  • Publication number: 20090165176
    Abstract: Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
    Type: Application
    Filed: July 29, 2008
    Publication date: June 25, 2009
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Evgueni Ananiev, Olga N. Danilevskaya, Mark A. Chamberlin, William J. Gordon-Kamm, Sergei Svitashev, Chengcang C. Wu
  • Publication number: 20090100550
    Abstract: Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
    Type: Application
    Filed: June 20, 2008
    Publication date: April 16, 2009
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Evgueni Ananiev, Mark A. Chamberlin, William J. Gordon-Kamm, Sergei Svitashev, Chengcang Wu
  • Publication number: 20070271629
    Abstract: Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C (CENPC) and methods for making such minichromosomes are described.
    Type: Application
    Filed: May 17, 2007
    Publication date: November 22, 2007
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Evgueni Ananiev, Mark A. Chamberlin, William J. Gordon-Kamm, Sergei Svitashev, Chengcang Wu