Patents by Inventor Shalini Gohil
Shalini Gohil has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240125772Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.Type: ApplicationFiled: November 7, 2023Publication date: April 18, 2024Inventors: Joshua Delaney, Shalini Gohil, Jill Herschleb, Adam Lowe, Albert Kim, Meiliana Tjandra
-
Publication number: 20240117410Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.Type: ApplicationFiled: October 31, 2023Publication date: April 11, 2024Inventors: Joshua DELANEY, Shalini Gohil, Veronica Emelina Gonzalez Munoz, Joshua Gu, Albert Dale Kim, Yi Luo, Tathagata Mukherjee, Monica Nagendran, James Francis Perna, III, Kristen Nguyen Pham, Meiliana Tjandra
-
Patent number: 11926822Abstract: This disclosure relates to compositions and methods for three-dimensional spatial profiling of analytes in a biological sample. The methods include use of a hydrogel comprising one or more polymers that include a phenol moiety, an azide moiety, or an alkyne moiety.Type: GrantFiled: September 22, 2021Date of Patent: March 12, 2024Assignee: 10x Genomics, Inc.Inventors: Shalini Gohil, Eswar Prasad Ramachandran Iyer
-
Publication number: 20240035071Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.Type: ApplicationFiled: June 16, 2023Publication date: February 1, 2024Applicant: 10X Genomics, Inc.Inventors: Joshua DELANEY, Shalini Gohil, Veronica Emelina Gonzalez Muñoz, Joshua Gu, Albert Dale Kim, Yi Luo, Tathagata Mukherjee, Monica Nagendran, James Francis Perna, III, Kristen Nguyen Pham, Meiliana Tjandra
-
Patent number: 11884964Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: July 11, 2022Date of Patent: January 30, 2024Assignee: 10X GENOMICS, INC.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
-
Publication number: 20230304072Abstract: The present disclosure relates generally to compositions and methods for generating hydrogel coatings on features. In some embodiments, hydrogel-coated features are prepared by horseradish peroxidase-mediated gelation of microspheres. Some embodiments relate to methods for generating hydrogel coatings using particle templated emulsification. The disclosure also relates to the use of hydrogel-coated features in arrays for spatial analysis of biological analytes in biological samples.Type: ApplicationFiled: March 22, 2023Publication date: September 28, 2023Inventors: Shalini GOHIL, Shea Thompson LANCE, Geoffrey P. MCDERMOTT, David Michael PATTERSON, Andrew D. PRICE
-
Publication number: 20230295556Abstract: The present disclosure provides methods, compositions, and systems for selective enzymatic gelation of cells, e.g., immune cells, contained in partitions.Type: ApplicationFiled: March 22, 2023Publication date: September 21, 2023Inventors: Shalini GOHIL, Joshua DELANEY, Yi LUO, Adam LOWE, Jason C. BELL, Michael John Terry STUBBINGTON, Wyatt James MCDONNELL
-
Publication number: 20230279481Abstract: The present disclosure provides compositions, methods, and kits comprising a nano-partition contained within a partition that allows for the separation and use of at least two enzymes in a partition-based assay of a biological sample, such as a single cell in a droplet. The compositions and methods are useful for carrying out partition-based assays of fixed biological samples.Type: ApplicationFiled: May 12, 2023Publication date: September 7, 2023Inventors: Sean Marrache, Shalini Gohil, Joshua Delaney, Paul Eugene Lund
-
Publication number: 20220364150Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: July 11, 2022Publication date: November 17, 2022Applicant: 10x Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
-
Publication number: 20220334031Abstract: The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.Type: ApplicationFiled: June 21, 2022Publication date: October 20, 2022Applicant: 10x Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Adam Lowe, Yi Luo, Dagmar Walter
-
Patent number: 11441172Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: September 1, 2020Date of Patent: September 13, 2022Assignee: 10x Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
-
Publication number: 20220049293Abstract: Provided herein are methods of determining a location of a biological analyte in a biological sample.Type: ApplicationFiled: December 6, 2019Publication date: February 17, 2022Applicant: 10x Genomics, Inc.Inventors: Lucas Frenz, Eswar Prasad Ramachandran Iyer, Tarjei Sigurd Mikkelsen, Preyas Shah, Katherine Pfeiffer, Yifeng Yin, Elliott Meer, Jessica Michele Terry, Yi Luo, Shalini Gohil, Joshua Delaney, Adam Lowe, Linda Kvastad, Eva Gracia Villacampa, Ludvig Larsson, Joakim Lundeberg, Zachary Bent, Meghan L. F. Frey, Jennifer Chew
-
Publication number: 20210190770Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.Type: ApplicationFiled: December 22, 2020Publication date: June 24, 2021Applicant: 10X Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Jill HERSCHLEB, Adam LOWE, Albert KIM, Meiliana TJANDRA
-
Publication number: 20200392564Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: September 1, 2020Publication date: December 17, 2020Applicant: 10X Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
-
Patent number: 10837047Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: April 3, 2019Date of Patent: November 17, 2020Assignee: 10X Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
-
Patent number: 10774369Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: April 3, 2019Date of Patent: September 15, 2020Assignee: 10X Genomics, Inc.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price, Joseph Francis Shuga
-
Patent number: 10590244Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: GrantFiled: November 1, 2018Date of Patent: March 17, 2020Assignee: 10X GENOMICS, INC.Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price
-
Publication number: 20190233878Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: April 3, 2019Publication date: August 1, 2019Applicant: 10X Genomics, Inc.Inventors: Joshua DELANEY, Shalini GOHIL, Christopher HINDSON, Adam LOWE, Andrew D. PRICE, Joseph Francis SHUGA
-
Publication number: 20190100632Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.Type: ApplicationFiled: November 1, 2018Publication date: April 4, 2019Inventors: Joshua Delaney, Shalini Gohil, Christopher Hindson, Adam Lowe, Andrew D. Price