Abstract: Microfluidic devices, kits systems and methods are provided for high throughput phenotypic separation of magnetically labelled cell samples. The devices and methods can be used for example to sort cells based on level of target marker and to sort screen cells, such as CRISPR screen cells and other screens with a large number of target cells, to isolate target cells and putative genetic modifiers.

Abstract: Methods and devices for detecting metabolic activity of target cells in a sample. The target cells are concentrated in a nanoliter well having a microfilter. A reporter compound that exhibits a change in electrochemical state in response to metabolic activity of the target cells is introduced. Metabolic activity or viability of the target cells is detected based on a determined change in the electrochemical state of contents in the well.

Abstract: Methods and devices for detecting metabolic activity of target cells in a sample. The target cells are concentrated in a nanoliter well having a microfilter. A reporter compound that exhibits a change in electrochemical state in response to metabolic activity of the target cells is introduced. Metabolic activity or viability of the target cells is detected based on a determined change in the electrochemical state of contents in the well.

Abstract: Methods and devices for detecting metabolic activity of target cells in a sample. The target cells are concentrated in a nanoliter well having a microfilter. A reporter compound that exhibits a change in electrochemical state in response to metabolic activity of the target cells is introduced. Metabolic activity or viability of the target cells is detected based on a determined change in the electrochemical state of contents in the well.

Abstract: Methods and devices for detecting metabolic activity of target cells in a sample. The target cells are concentrated in a nanoliter well having a microfilter. A reporter compound that exhibits a change in electrochemical state in response to metabolic activity of the target cells is introduced. Metabolic activity or viability of the target cells is detected based on a determined change in the electrochemical state of contents in the well.

Abstract: Devices and methods for capture of target particles in a flow. There is a plurality of flow rate-reducing structures in a flow chamber, each structure including a trapping surface shaped to reduce flow rate in a vicinity of the trapping surface. Reduced flow rate in the vicinity of the trapping surface is non-zero and has a magnitude lower than that of flow rate in other regions of the flow chamber. The reduced flow rate is sufficiently low for an attraction force acting on the target particles to overcome drag force on the target particles, to promote capture of particles in the vicinity of the trapping surface. The device may exhibit different sorting zones for capturing particles that experience different amounts and/or types of attraction force. The device may enable sorting of cells according to their level of display of specific protein surface markers.

Abstract: Devices and methods for capture of target particles in a flow. There is a plurality of flow rate-reducing structures in a flow chamber, each structure including a trapping surface shaped to reduce flow rate in a vicinity of the trapping surface. Reduced flow rate in the vicinity of the trapping surface is non-zero and has a magnitude lower than that of flow rate in other regions of the flow chamber. The reduced flow rate is sufficiently low for an attraction force acting on the target particles to overcome drag force on the target particles, to promote capture of particles in the vicinity of the trapping surface. The device may exhibit different sorting zones for capturing particles that experience different amounts and/or types of attraction force. The device may enable sorting of cells according to their level of display of specific protein surface markers.