Abstract: Disclosed is a marker for observing an effect of a compound or a drug on cells in real time. The marker is: 1) an amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2; or 2) an amino acid sequence having a function for observing an effect of a compound or a drug on cells in real time and having at least more than 80%, preferably more than 85%, more preferably 90%, further preferably 95%, and most preferably 99% homology with the amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2. Also disclosed is a method and a kit for observing an effect of a compound or a drug on cells in real time and use thereof.

Abstract: Disclosed is a marker for observing an effect of a compound or a drug on cells in real time. The marker is: 1) an amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2; or 2) an amino acid sequence having a function for observing an effect of a compound or a drug on cells in real time and having at least more than 80%, preferably more than 85%, more preferably 90%, further preferably 95%, and most preferably 99% homology with the amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2.

Abstract: The invention relates to the usage of Exocyst complex component or Sec3 or its homolog as a molecular marker for monitoring an exogenous molecule like nucleic acid in transit and optimizing the delivery of the exogenous molecule to a cell. Delivery of the molecule is monitored by detecting expression of an Exocyst complex component or Sec3 or its homolog in said cell, wherein the expression of the Exocyst complex component or Sec 3 or its homolog indicates that the cell is competent to receive the exogenous molecule. The invention may be used (a) to determine the efficiency of delivery; (b) to control the copy number of DNA delivery; (c) to identify the cells transformed with an exogenous DNA; (d) to identify molecular markers associated with transformation competency of a cell; (e) identifying, characterizing and producing cells competent to receive exogenous nucleic acid.

Abstract: The present invention provides an Agrobacterium tumefaciens outer membrane protein designated aopB, its variants, homologs and fragments and corresponding DNA molecules. The invention also relates to a method whereby aopB.related proteins are used as carriers to display passenger proteins on the surface of bacteria. Display of the passenger protein permits applicatins such as live vaccine development, library screening, protein purification, bio-decontainment, and whole cell catalysis.

Abstract: The present invention provides an Agrobacterium tumefaciens outer membrane protein designated aopB, its variants, homologs and fragments and corresponding DNA molecules. The invention also relates to a method whereby aopB.related proteins are used as carriers to display passenger proteins on the surface of bacteria.

Abstract: This invention relates to methods and kits for determining the level of H2O2 inside a cell, and for determining whether a test compound has ability to scavenge a reactive oxygen species (ROS). The methods and diagnostic kits of this invention employ a cell containing a promoter which is inducible by an ROS, such as the H2O2-inducible KatA promoter of Agrobacterium tumefacines; the promoter is used to drive expression of a reporter gene. The expression level of the reporter gene correlates with the level of ROS, specifically H2O2, inside the cell. When the cell is exposed to a test compound, either intra-cellularly or extra-cellularly, if the level of ROS-inducible expression of the reporter is reduced, then this indicates that the test compound is a scavenger of the ROS.