Patents by Inventor Shinya Kurata
Shinya Kurata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11935944Abstract: The on-state characteristics of a transistor are improved and thus, a semiconductor device capable of high-speed response and high-speed operation is provided. A highly reliable semiconductor device showing stable electric characteristics is made. The semiconductor device includes a transistor including a first oxide layer; an oxide semiconductor layer over the first oxide layer; a source electrode layer and a drain electrode layer in contact with the oxide semiconductor layer; a second oxide layer over the oxide semiconductor layer; a gate insulating layer over the second oxide layer; and a gate electrode layer over the gate insulating layer. An end portion of the second oxide layer and an end portion of the gate insulating layer overlap with the source electrode layer and the drain electrode layer.Type: GrantFiled: September 2, 2022Date of Patent: March 19, 2024Assignee: Semiconductor Energy Laboratory Co., Ltd.Inventors: Shunpei Yamazaki, Hideomi Suzawa, Shinya Sasagawa, Motomu Kurata, Masashi Tsubuku
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Publication number: 20180087096Abstract: Provided are techniques by which a mutant gene in a gene group comprising a large number of wild-type genes can be detected with high sensitivity and in a rapid and simple way. Provided is a method for measuring a nucleic acid for the purpose of specifically detecting a genotype as an examination target from subjects which are genes or specimens likely to have a plurality of genetic polymorphisms, wherein the measurement method comprises hybridizing a fluorescent dye-labeled oligonucleotide with a genotype other than the examination target to suppress the gene amplification of such genotype while at the same time, hybridizing the same fluorescence-labeled oligo as described above with an amplification product derived from the genotype of the examination target amplified in the same gene amplification step as described above, and specifically detecting the genotype of the examination target based on a change in the fluorescence intensity of the fluorescent dye before and after the hybridization.Type: ApplicationFiled: March 29, 2016Publication date: March 29, 2018Applicant: NIPPON STEEL & SUMIKIN ECO-TECH CORPORATIONInventors: Masahiro YAMAGUCHI, Shinya KURATA, Norio KOMATSU, Soji MORISHITA
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Patent number: 9587271Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: GrantFiled: October 31, 2012Date of Patent: March 7, 2017Assignee: NIPPON STEEL & SUMIKIN ECO-TECH CORPORATIONInventors: Kazunori Nakamura, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20150368712Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.Type: ApplicationFiled: September 8, 2015Publication date: December 24, 2015Inventors: Yuji SEKIGUCHI, Naohiro NODA, Ryo MIYATA, Kazunori NAKAMURA, Shinya KURATA, Satoshi TSUNEDA, Hidenori TANI
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Publication number: 20140248611Abstract: Objects are to provide nucleic acid probes, which in the detection of target nucleic acids with fluorescent quenching probes, can assay a greater variety of target nucleic acids at the same time while suppressing increases in assay labor and cost, and also a method for assaying target nucleic acids by using the nucleic acid probes. Provided is a nucleic acid probe having a base sequence hybridizable with a target nucleic acid sequence in a target nucleic acid.Type: ApplicationFiled: June 18, 2012Publication date: September 4, 2014Applicant: NIPPON STEEL & SUMIKIN ECO-TECH CORPORATIONInventors: Kohei Ichikawa, Akiyoshi Hanawa, Shinya Kurata
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Publication number: 20140178876Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.Type: ApplicationFiled: December 2, 2013Publication date: June 26, 2014Applicant: Nippon Steel & Sumikin Eco-Tech CorporationInventors: Yuji SEKIGUCHI, Naohiro NODA, Ryo MIYATA, Kazunori NAKAMURA, Shinya KURATA, Satoshi TSUNEDA, Hidenori TANI
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Publication number: 20130065237Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: October 31, 2012Publication date: March 14, 2013Inventors: Kazunori NAKAMURA, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20110224417Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: April 19, 2011Publication date: September 15, 2011Applicants: KANKYO ENGINEERING CO., LTD., NATIONAL INSTITUTE OF ADV. INDUSTRIAL SCI. & TECH.Inventors: KAZUNORI NAKAMURA, TAKAHIRO KANAGAWA, NAOHIRO NODA, SATOSHI TSUNEDA, HIDENORI TANI, SHINYA KURATA
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Publication number: 20110212442Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, which can hybridize to the fluorescent probe (A), and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid sequence (C). The fluorescent substance (d) is a fluorescent substance which changes in fluorescent character upon interaction with guanine. At least one of nucleotide units which constitute the fluorescent probe (A) is an artificial nucleotide unit having a function to raise a dissociation temperature between the probe (A) and the fluorescent probe binding region (b1). The nucleic acid probe is provided with an improved fluorescence quenching efficiency.Type: ApplicationFiled: July 30, 2009Publication date: September 1, 2011Applicant: Nippon Steel Kankyo Engineering Co., Ltd.Inventors: Yuji Sekiguchi, Naohiro Noda, Ryo Miyata, Kazunori Nakamura, Shinya Kurata, Satoshi Tsuneda, Hidenori Tani
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Publication number: 20110189666Abstract: A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide including (a) a nucleotide labeled with a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, to which the fluorescent probe (A) can hybridize, and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid (C). The nucleic acid probe set is designed such that, when the fluorescent probe (A) hybridizes to the fluorescent probe binding region (b1) and the target nucleic acid (C) hybridizes to the target nucleic acid binding region (b2), guanine in the target nucleic acid (C) and the fluorescent substance (d) labeled on the nucleotide (a) interact with each other to change a fluorescent character of the fluorescent substance (d).Type: ApplicationFiled: July 30, 2008Publication date: August 4, 2011Applicant: Nippon Steel Kankyo Engineering Co., LtdInventors: Kohei Ichikawa, Kazunori Nakamura, Shinya Kurata
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Patent number: 7951604Abstract: To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: GrantFiled: August 13, 2009Date of Patent: May 31, 2011Assignees: Kankyo Engineering Co., Ltd., National Institute of Advanced Industrial Science and TechnologyInventors: Kazunori Nakamura, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Patent number: 7785795Abstract: A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system.Type: GrantFiled: April 10, 2009Date of Patent: August 31, 2010Assignees: Kankyo Engineering Co., Ltd., National Institute of Advanced Industrial Science and TechnologyInventors: Shinya Kurata, Kyoko Takatsu, Kazunori Nakamura, Takahiro Kanagawa
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Publication number: 20100015719Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: August 13, 2009Publication date: January 21, 2010Applicants: KANKYO ENGINEERING Co., Ltd., National Institute of Adv. Industrial Sci. & Tech.Inventors: Kazunori NAKAMURA, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Publication number: 20090233308Abstract: A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with at least one label selected from the group consisting of (a) fluorescent dyes, (b) quenchers and (c) immune related substances with a fluorescent dye or quencher contained therein, and (C) at least one nucleic acid-synthesizing enzyme. The template nucleic acid or a nucleic acid, which has been synthesized using the template nucleic acid as a template, is then assayed from a change or an amount of a change in an optical character of the nucleic acid polymerization system.Type: ApplicationFiled: April 10, 2009Publication date: September 17, 2009Applicants: KANKYO ENGINEERING Co., Ltd., Nat Institute of Advance Indust Science & Tech.Inventors: Shinya KURATA, Kyoko Takatsu, Kazunori Nakamura, Takahiro Kanagawa
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Patent number: 7429655Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.Type: GrantFiled: October 14, 2003Date of Patent: September 30, 2008Assignees: Kankyo Engineering Co., Ltd., National Institute of Advanced Industrial Science and TechnologyInventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
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Patent number: 7419786Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.Type: GrantFiled: January 27, 2006Date of Patent: September 2, 2008Assignees: Japan Bioindustry Association, Japan as represented by Secretary of Agency of Industrial Science and Technology, KANKYO ENGINEERING Co., Ltd.Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
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Patent number: 7354707Abstract: Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.Type: GrantFiled: June 27, 2001Date of Patent: April 8, 2008Assignees: National Institute of Advanced Industrial Science and Technology, Kankyo Engineering Co., Ltd.Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
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Patent number: 7273700Abstract: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.Type: GrantFiled: March 27, 2002Date of Patent: September 25, 2007Assignees: National Institute of Advanced Industrial Science and Technology, Kankyo Engineering Co., Ltd.Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
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Publication number: 20070128608Abstract: [Problems] To provide a novel mixture for assaying a target nucleic acid, characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; 2) requiring no procedure of changing a probe concentration depending on a concentration of the target nucleic acid. [Means for Solving Problems] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with a fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with a fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and one double nucleic acid probe labeled with two fluorescent dyes; and a method for assaying a nucleic acid by making use thereof.Type: ApplicationFiled: December 20, 2004Publication date: June 7, 2007Applicants: Kankyo Engineering Co., Ltd., National Institute of Adv. Industrial Sci. & Tech.Inventors: Kazunori Nakamura, Takahiro Kanagawa, Naohiro Noda, Satoshi Tsuneda, Hidenori Tani, Shinya Kurata
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Patent number: 7094540Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.Type: GrantFiled: August 1, 2002Date of Patent: August 22, 2006Assignees: Japan Bioindustry Association, Agency of Industrial Science and Technology, Kankyo Engineering Co., Ltd.Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho