Patents by Inventor Shiro Fukuyama
Shiro Fukuyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240124902Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.Type: ApplicationFiled: November 21, 2023Publication date: April 18, 2024Applicant: Novozymes A/SInventors: Michael Glenn Catlett, Monica Tassone, Paul Vincent Harris, Robert Lyle Osborne, Shiro Fukuyama, Tomoko Matsul, Ryoko Kataoka
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Publication number: 20240101991Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.Type: ApplicationFiled: September 28, 2022Publication date: March 28, 2024Applicant: NOVOZYMES A/SInventors: Marc Dominique Morant, Tomoko Matsui, Kirk Matthew Schnorr, Shiro Fukuyama, Noriko Tsutsumi, Zhengfang Kang
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Patent number: 11866751Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.Type: GrantFiled: July 22, 2019Date of Patent: January 9, 2024Assignee: Novozymes A/SInventors: Michael Glenn Catlett, Monica Tassone, Paul Vincent Harris, Robert Lyle Osborne, Ryoko Kataoka, Shiro Fukuyama, Tomoko Matsui
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Publication number: 20230235367Abstract: The present invention relates to raw starch hydrolysis and in particular to a raw starch hydrolysis and fermentation process.Type: ApplicationFiled: February 9, 2021Publication date: July 27, 2023Applicant: Novozymes A/SInventors: Carsten Andersen, Thomas Agersten Poulsen, Dorota Nissen, Marie Thryøse Kruse, Sarah Schultheis Elliott, Shiro Fukuyama
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Patent number: 11667938Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.Type: GrantFiled: September 2, 2021Date of Patent: June 6, 2023Assignee: Novozymes A/SInventors: Shiro Fukuyama, Keiichi Ayabe
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Publication number: 20230142991Abstract: The present invention relates to isolated alpha-amylase variants of a parent alpha-amylase, comprising an alteration at one or more positions corresponding to positions 196, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 28, 38, 39, 43, 54, 56, 57, 64, 67, 68, 70, 71, 86, 89, 90, 94, 96, 99, 101, 103, 107, 108, 110, 113, 114, 117, 127, 134, 138, 142, 150, 151, 152, 156, 169, 171, 174, 179, 183, 193, 199, 200, 204, 205, 207, 208, 209, 212, 218, 221, 222, 224, 233, 241, 245, 259, 275, 278, 281, 282, 283, 284, 285, 308, 323, 335, 348, 359, 382, 386, 388, 392, 394, 396, 412, 414, 417, 424, 428, 457, 459, 466, 479, 489, 511, 533, 534, 542, 543, 545, 547, 549, 550, 551, 560, 566, 570, 574, 575, 576, 577, 578, 580, 581, 582, 589, 592, 599, 603, 605, 608, 614, 619, or 626 of the polypeptide of SEQ ID NO: 1, wherein the variant has alpha-amylase activity and wherein the variant has at least 60%, e.g.Type: ApplicationFiled: February 9, 2021Publication date: May 11, 2023Applicant: Novozymes A/SInventors: Carsten Andersen, Thomas Agersten Poulsen, Dorota Nissen, Marie Thryøse Kruse, Sarah Schultheis Elliott, Shiro Fukuyama
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Patent number: 11584783Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.Type: GrantFiled: June 26, 2018Date of Patent: February 21, 2023Assignee: NOVOZYMES A/SInventors: Marc Dominique Morant, Tomoko Matsui, Kirk Matthew Schnorr, Shiro Fukuyama, Noriko Tsutsumi, Zhengfang Kang
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Patent number: 11377648Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6.Type: GrantFiled: April 8, 2019Date of Patent: July 5, 2022Assignee: NOVOZYMES A/SInventors: Tomoko Namoto, Takashi Nakanishi, Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe
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Patent number: 11279920Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: September 19, 2018Date of Patent: March 22, 2022Assignee: NOVOZYMES A/SInventors: Ming Li, Junxin Duan, Zheng Liu, Shiro Fukuyama, Keiichi Ayabe, Guillermo Coward-Kelly, Randall Deinhammer
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Publication number: 20210395791Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.Type: ApplicationFiled: September 2, 2021Publication date: December 23, 2021Applicant: Novozymes A/SInventors: Shiro Fukuyama, Keiichi Ayabe
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Patent number: 11136607Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.Type: GrantFiled: May 6, 2019Date of Patent: October 5, 2021Assignee: Novozymes A/SInventors: Shiro Fukuyama, Keiichi Ayabe
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Publication number: 20210261988Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.Type: ApplicationFiled: July 22, 2019Publication date: August 26, 2021Applicant: Novozymes A/SInventors: Michael Glenn Catlett, Monica Tassone, Paul Vincent Harris, Robert Lyle Osborne, Ryoko Kataoka, Shiro Fukuyama, Tomoko Matsui
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Publication number: 20210139875Abstract: The present invention relates to a hybrid polypeptide having alpha-amylase activity, selected from a first polypeptide sequence comprising a catalytic core, and a second polypeptide sequence comprising a carbohydrate binding module (CBM), wherein (a) the catalytic core is selected from a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to amino acids 20 to 494 of SEQ ID NO: 1 or amino acids 20 to 496 of SEQ ID NO: 1; and (b) the CBM is selected from a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6.Type: ApplicationFiled: April 8, 2019Publication date: May 13, 2021Applicant: NOVOZYMES A/SInventors: Tomoko Namoto, Takashi Nakanishi, Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe
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Patent number: 10800999Abstract: The present invention relates to method for production of brewer's wort comprising adding to a mash, a particular glucoamylase. The glucoamylase is obtained from Penicillium oxalicum. Further, the invention relates to use of a particular glucoamylase for production of brewer's wort. Furthermore, the invention relates to use of a combination of a glucoamylase and a pullulanase for production of brewer's wort.Type: GrantFiled: April 11, 2012Date of Patent: October 13, 2020Assignee: Novozymes A/SInventors: Anne Mette Bhatia Frederiksen, Shiro Fukuyama, Keiichi Ayabe
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Publication number: 20200248208Abstract: The present invention provides in a process of producing ethanol from starch-containing material comprising: (a) saccharifying the starch-containing material; and (b) fermenting using a fermentation organism; wherein saccharification and/or fermentation is done in the presence of at least a glucoamyl ase and optionally an alpha-amylase; the fermenting organism is Saacaromyces cerevisiae; and wherein a glucoamylase and/or an alpha-amylase is expressed from the fermenting organism. Further the invention provides yeast strains specifically developed for the process of the invention.Type: ApplicationFiled: November 14, 2016Publication date: August 6, 2020Applicant: Novozymes A/SInventors: Michael G. Catlett, Jennifer Headman, Shiro Fukuyama
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Publication number: 20200172946Abstract: The present invention relates to a method of making glucose syrup from liquefied starch, and to compositions useful therein.Type: ApplicationFiled: February 12, 2020Publication date: June 4, 2020Applicant: Novozymes A/SInventors: Shiro Fukuyama, Keiichi Ayabe
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Publication number: 20200165591Abstract: Improved processes for producing ethanol from starch-containing materials by the combined use of at least one endoprotease and at least one exo-protease in an SSF process are disclosed. More particularly the exo-protease should make up at least 5% (w/w) of the protease mixture.Type: ApplicationFiled: March 1, 2017Publication date: May 28, 2020Applicant: NOVOZYMES A/SInventors: Chee-Leong Soong, Bernardo Vidal Jr., Shiro Fukuyama, Tomoko Matsui, Keiichi Ayabe, Louis Patrick Lessard, Nathaniel E. Kreel, Kenneth Jensen, Ming Li, Wei Peng, Lan Tang, Xinyan Guo
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Publication number: 20200140495Abstract: The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.Type: ApplicationFiled: June 26, 2018Publication date: May 7, 2020Applicant: NOVOZYMES A/SInventors: Marc Dominique Morant, Tomoko Matsui, Kirk Matthew Schnorr, Shiro Fukuyama, Noriko Tsutsumi, Zhengfang Kang
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Patent number: 10584324Abstract: The present invention relates to glucoamylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: October 23, 2015Date of Patent: March 10, 2020Assignee: NOVOZYMES A/SInventors: Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe
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Patent number: 10538752Abstract: The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.Type: GrantFiled: August 17, 2017Date of Patent: January 21, 2020Assignee: NOVOZYMES A/SInventors: Guillermo Coward-Kelly, Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe