Patents by Inventor Shoen Kume
Shoen Kume has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11692173Abstract: The present invention provides, in order to prepare matured hepatocytes analogous in various points to primary hepatocytes, a method for preparing hepatocytes or cells that can be differentiated into hepatocytes from pluripotent stem cells, comprising the steps of: (1) culturing the pluripotent stem cells in a medium containing an activator of an activin receptor-like kinase-4,7; (2) culturing the cells obtained in the step (1) in a medium containing a bone morphogenetic factor and a fibroblast growth factor; (3) culturing the cells obtained in the step (2) in a medium containing an activator of a hepatocyte growth factor receptor and an activator of an oncostatin M receptor; and (4) culturing the cells obtained in the step (3) to obtain hepatocytes or cells that can be differentiated into hepatocytes, wherein in at least one of the steps (2), (3) and (4), cells are cultured on a high-density collagen gel membrane.Type: GrantFiled: October 9, 2018Date of Patent: July 4, 2023Assignees: TOKYO INSTITUTE OF TECHNOLOGY, KANTO KAGAKU KABUSHIKI KAISHA, CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Shoen Kume, Nobuaki Shiraki, Hiroyuki Yamaguchi, Tomoaki Inoue, Toshito Nakagawa, Jumpei Kiyokawa
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Publication number: 20220339260Abstract: As an approach of efficiently inducing differentiation from pluripotent stem cells into insulin-producing cells, provided is a method comprising the step of three-dimensionally culturing cells in a medium containing a dihydroindolizinone derivative.Type: ApplicationFiled: July 7, 2020Publication date: October 27, 2022Applicants: TOKYO INSTITUTE OF TECHNOLOGY, DAIICHI SANKYO COMPANY, LIMITEDInventors: Shoen Kume, Nobuaki Shiraki, Tatsuya Yano, Toshihiro Kiho
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Publication number: 20210340500Abstract: As a method for producing enterocytes derived from pluripotent stem cells and having functions corresponding to those of actual enterocytes, a method for producing enterocytes using an enterocyte differentiation medium containing a GSK3 inhibitor, and at least one selected from the group consisting of an activator of a hepatocyte growth factor receptor, an adrenal cortex hormone, calcitriol, and dimethyl sulfoxide is provided.Type: ApplicationFiled: September 9, 2019Publication date: November 4, 2021Applicants: TOKYO INSTITUTE OF TECHNOLOGY, THE UNIVERSITY OF TOKYO, KANTO KAGAKU KABUSHIKI KAISHAInventors: Shoen KUME, Nobuaki SHIRAKI, Kazuya MAEDA, Hiroyuki KUSUHARA, Masaya ISHIKAWA, Teruhiko WATANABE
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Publication number: 20210207085Abstract: The present invention provides, in order to prepare matured hepatocytes analogous in various points to primary hepatocytes, a method for preparing hepatocytes or cells that can be differentiated into hepatocytes from pluripotent stem cells, comprising the steps of: (1) culturing the pluripotent stem cells in a medium containing an activator of an activin receptor-like kinase-4,7; (2) culturing the cells obtained in the step (1) in a medium containing a bone morphogenetic factor and a fibroblast growth factor; (3) culturing the cells obtained in the step (2) in a medium containing an activator of a hepatocyte growth factor receptor and an activator of an oncostatin M receptor; and (4) culturing the cells obtained in the step (3) to obtain hepatocytes or cells that can be differentiated into hepatocytes, wherein in at least one of the steps (2), (3) and (4), cells are cultured on a high-density collagen gel membrane.Type: ApplicationFiled: October 9, 2018Publication date: July 8, 2021Applicants: TOKYO INSTITUTE OF TECHNOLOGY, KANTO KAGAKU KABUSHIKI KAISHA, CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Shoen KUME, Nobuaki SHIRAKI, Hiroyuki YAMAGUCHI, Tomoaki INOUE, Toshito NAKAGAWA, Jumpei KIYOKAWA
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Publication number: 20210115397Abstract: In order to improve the efficiency of inducing differentiation of pluripotent stem cells, provided is a method for promoting differentiation of pluripotent stem cells, the method including a step of culturing pluripotent stem cells in a medium, wherein the medium is a medium containing a) an insulin-like growth factor, b) an insulin analogue preparation containing no zinc, c) an insulin analogue preparation containing a low concentration of zinc, or d) a compound exhibiting an insulin-like action.Type: ApplicationFiled: April 25, 2019Publication date: April 22, 2021Applicants: TOKYO INSTITUTE OF TECHNOLOGY, Ajinomoto Co., Inc.Inventors: Shoen KUME, Nobuaki SHIRAKI, Akira CHIBA, Takayuki ENOMOTO
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Patent number: 10457916Abstract: It is an object of the present invention to provide a method for efficiently directing differentiation into insulin-producing cells in a xeno-free culture system.Type: GrantFiled: May 19, 2015Date of Patent: October 29, 2019Assignee: Tokyo Institute of TechnologyInventors: Shoen Kume, Nobuaki Shiraki
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Patent number: 10000739Abstract: The purpose of the present invention is to provide a method and a culture medium which are capable of efficiently inducing the differentiation of pluripotent stem cells such as ES cells and iPS cells into desired cells by a simple means. The differentiation of pluripotent stem cells can be induced by culturing mammal-derived pluripotent stem cells in a differentiation culture medium that does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine. Also provided is a differentiation-inducing culture medium which does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine.Type: GrantFiled: October 20, 2011Date of Patent: June 19, 2018Assignee: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITYInventors: Shoen Kume, Fumio Endo, Nobuaki Shiraki, Yasuko Shiraki, Kazuhiko Kume
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Patent number: 9617517Abstract: A purpose of the present invention is to provide a small chemical compound which especially promotes induction of the differentiation of ES cells into insulin-producing cells. Another purpose of the present invention is to provide: a method for inducing the differentiation of ES cells into insulin-producing cells using the compound; and an agent for promoting induction of the differentiation into insulin-producing cells. Another purpose of the present invention is to provide the thus-induced insulin-producing cells. Provided are: an agent for promoting induction of the differentiation of stem cells derived from a mammal into insulin-producing cells, which contains a compound that is selected from the group consisting of dopamine metabolism inhibitors, serotonin metabolism inhibitors, acetylchotine, acetylcholine degrading enzyme inhibitors and acetylcholine receptor activators; and a method for inducing differentiation using the agent for promoting induction of differentiation.Type: GrantFiled: May 1, 2012Date of Patent: April 11, 2017Assignees: NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY, KYOTO UNIVERSITYInventors: Shoen Kume, Daisuke Sakano, Nobuaki Shiraki, Kahoko Umeda, Taiji Yamazoe, Kazuhiko Kume, Motonari Uesugi
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Publication number: 20170081639Abstract: It is an object of the present invention to provide a method for efficiently directing differentiation into insulin-producing cells in a xeno-free culture system.Type: ApplicationFiled: May 19, 2015Publication date: March 23, 2017Applicant: TOKYO INSTITUTE OF TECHNOLOGYInventors: Shoen KUME, Nobuaki SHIRAKI
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Patent number: 9376665Abstract: An object of the present invention is to provide a method of producing intestinal cells by use of pluripotent stem cells as a starting material. According to the present invention, provided is a method of producing intestinal cells, comprising the steps of: (A) inducing differentiation of pluripotent stem cells into definitive endoderm cells; and (B) culturing the definitive endoderm cells in the presence of (2?Z,3?E)-6-bromoindirubin-3?-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) to thereby induce differentiation of the definitive endoderm cells into intestinal cells.Type: GrantFiled: October 31, 2011Date of Patent: June 28, 2016Assignees: National University Corporation Kumamoto University, LSIP, LLCInventors: Shoen Kume, Soichiro Ogaki, Nobuaki Shiraki, Kazuhiko Kume
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Publication number: 20140256043Abstract: A purpose of the present invention is to provide a small chemical compound which especially promotes induction of the differentiation of ES cells into insulin-producing cells. Another purpose of the present invention is to provide: a method for inducing the differentiation of ES cells into insulin-producing cells using the compound; and an agent for promoting induction of the differentiation into insulin-producing cells. Another purpose of the present invention is to provide the thus-induced insulin-producing cells. Provided are: an agent for promoting induction of the differentiation of stem cells derived from a mammal into insulin-producing cells, which contains a compound that is selected from the group consisting of dopamine metabolism inhibitors, serotonin metabolism inhibitors, acetylchotine, acetylcholine degrading enzyme inhibitors and acetylcholine receptor activators; and a method for inducing differentiation using the agent for promoting induction of differentiation.Type: ApplicationFiled: May 1, 2012Publication date: September 11, 2014Applicants: Kyoto University, National University Corporation Kumamoto UniversityInventors: Shoen Kume, Daisuke Sakano, Nobuaki Shiraki, Kahoko Umeda, Taiji Yamazoe, Kazuhiko Kume, Motonari Uesugi
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Publication number: 20140199700Abstract: An object of the present invention is to provide a method of producing intestinal cells by use of pluripotent stem cells as a starting material. According to the present invention, provided is a method of producing intestinal cells, comprising the steps of: (A) inducing differentiation of pluripotent stem cells into definitive endoderm cells; and (B) culturing the definitive endoderm cells in the presence of (2?Z,3?E)-6-bromoindirubin-3?-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) to thereby induce differentiation of the definitive endoderm cells into intestinal cells.Type: ApplicationFiled: October 31, 2011Publication date: July 17, 2014Applicant: LSIP, LLCInventors: Shoen Kume, Soichiro Ogaki, Nobuaki Shiraki, Kazuhiko Kume
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Publication number: 20130252335Abstract: The purpose of the present invention is to provide a method and a culture medium which are capable of efficiently inducing the differentiation of pluripotent stem cells such as ES cells and iPS cells into desired cells by a simple means. The differentiation of pluripotent stem cells can be induced by culturing mammal-derived pluripotent stem cells in a differentiation culture medium that does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine. Also provided is a differentiation-inducing culture medium which does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine.Type: ApplicationFiled: October 20, 2011Publication date: September 26, 2013Inventors: Shoen Kume, Fumio Endo, Nobuaki Shiraki, Yasuko Shiraki, Kazuhiko Kume
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Patent number: 8309353Abstract: It is an object of the present invention to establish a system for reliably differentiating an ES cell into a hepatic cell. The present invention provides a method for inducing the differentiation of an ES cell into a hepatic cell, which comprises, in the presence of an M15 cell, culturing a mammal-derived ES cell in the presence of activin and bFGF, and then culturing the ES cell in the presence of dexamethasone, HGF, and oncostatin M.Type: GrantFiled: May 30, 2008Date of Patent: November 13, 2012Assignee: Kumamoto UniversityInventors: Shoen Kume, Nobuaki Shiraki, Kahoko Umeda, Kazuhiko Kume
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Publication number: 20120058491Abstract: It is an object of the present invention to establish a system for reliably differentiating an ES cell into a hepatic cell. The present invention provides a method for inducing the differentiation of an ES cell into a hepatic cell, which comprises, in the presence of an M15 cell, culturing a mammal-derived ES cell in the presence of activin and bFGF, and then culturing the ES cell in the presence of dexamethasone, HGF, and oncostatin M.Type: ApplicationFiled: May 30, 2008Publication date: March 8, 2012Applicant: KUMAMOTO UNIVERSITYInventors: Shoen Kume, Nobuaki Shiraki, Kahoko Umeda, Kazuhiko Kume