Abstract: A method for specifically and efficiently quantifying the expression of targeted RNA variants with specific terminal sequences suitable to identify multiple isoforms bearing complex heterogeneity in terminal sequences by hybridizing a 5?-Dbs-adapter to the 5?-end of target RNAs, wherein the 5?-Dbs-adapter has a stem-loop structure whose protruding 5?-end base-pairs with the 5?-end of target RNAs, and wherein the loop region of 5?-Dbs-adapter contains a base-lacking spacer which will terminate reverse transcription in a subsequent step; hybridizing a 3?db-adapter to the 3?-end of target RNAs, wherein the 3?-db-adapter has a stem-loop structure whose protruding 3?-end base-pairs with the 3?-end of target RNAs; ligating both adapters with target RNAs by RN12 ligation to form a “dumbbell-like” structure; and, amplifying and quantifying the ligation product by RT-PCR.

Abstract: A method for specifically and efficiently quantifying the expression of targeted RNA variants with specific terminal sequences suitable to identify multiple isoforms bearing complex heterogeneity in terminal sequences by hybridizing a 5?-Dbs-adapter to the 5?-end of target RNAs, wherein the 5?-Dbs-adapter has a stem-loop structure whose protruding 5?-end base-pairs with the 5?-end of target RNAs, and wherein the loop region of 5?-Dbs-adapter contains a base-lacking spacer which will terminate reverse transcription in a subsequent step; hybridizing a 3?Db-adapter to the 3?-end of target RNAs, wherein the 3?-Db-adapter has a stem-loop structure whose protruding 3?-end base-pairs with the 3?-end of target RNAs; ligating both adapters with target RNAs by RN12 ligation to form a “dumbbell-like” structure; and, amplifying and quantifying the ligation product by RT-PCR.

Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for performing sequencing of 5?-htRNA in an RNA sample including obtaining a DNA library of 5?-htRNA and sequencing the DNA library of 5?-htRNAs in the RNA sample. The invention also teaches a method for quantifying 5?-htRNA half molecules in an RNA sample including (a) treating an RNA sample containing 5?-htRNAs half molecules having a 3? cyclic phosphate with a T4 polynucleotide kinase to form 3?-dephosphrylated 5?-tRNA halves, (b) 3?-AD ligating the 3?-dephosphrylated 5?-tRNA halves to form ligated RNA products, and (c) quantifying the ligated RNA products by RT-qPCR using a plurality of target primers and probes configured to simultaneously quantify at least two cP-containing 5?-tRNA half species. The treating of step (a) is simultaneously performed as one step in a single tube with the 3?-AD ligating of step (b).

Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for quantifying a 5?-htRNA; a method for quantifying a 3?-htRNA; a method for obtaining a DNA library of 5?-htRNAs and a DNA library of 5?-htRNAs obtained therefrom; and a method for obtaining a DNA library of 3?-htRNAs and a DNA library of 3?-htRNAs obtained therefrom. The invention also teaches a method for determining the presence or absence of a cancer cell in a biological sample; a method of diagnosing cancer in a subject; and a method of prognosing cancer in a subject.

Abstract: A method for quantifying individual mature tRNA species, comprising: incubating mature tRNA in a buffer to remove the amino acids from the 3? end; annealing a DNA/RNA stem-loop adapter; ligating the annealed hybrid stem-loop adapter to the mature tRNA; and amplifying and quantifying the ligation product by TaqMan qRT-PCR.

Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for quantifying a 5?-htRNA; a method for quantifying a 3?-htRNA; a method for obtaining a DNA library of 5?-htRNAs and a DNA library of 5?-htRNAs obtained therefrom; and a method for obtaining a DNA library of 3?-htRNAs and a DNA library of 3?-htRNAs obtained therefrom. The invention also teaches a method for determining the presence or absence of a cancer cell in a biological sample; a method of diagnosing cancer in a subject; and a method of prognosing cancer in a subject.

Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for quantifying a 5?-htRNA; a method for quantifying a 3?-htRNA; a method for obtaining a DNA library of 5?-htRNAs and a DNA library of 5?-htRNAs obtained therefrom; and a method for obtaining a DNA library of 3?-htRNAs and a DNA library of 3?-htRNAs obtained therefrom. The invention also teaches a method for determining the presence or absence of a cancer cell in a biological sample; a method of diagnosing cancer in a subject; and a method of prognosing cancer in a subject.

Abstract: A method for quantifying individual mature tRNA species, comprising: incubating mature tRNA in a buffer to remove the amino acids from the 3? end; annealing a DNA/RNA stem-loop adapter; ligating the annealed hybrid stem-loop adapter to the mature tRNA; and amplifying and quantifying the ligation product by TaqMan qRT-PCR.

Abstract: A method for specifically and efficiently quantify the expression of targeted RNA variants with specific terminal sequences suitable to identify multiple isoforms bearing complex heterogeneity in terminal sequences by hybridizing a 5?-Dbs-adapter to the 5?-end of target RNAs, wherein the 5?-Dbs-adapter has a stem-loop structure whose protruding 5?-end base-pairs 5?-end of target RNAs, and wherein the loop region of 5?-Dbs-adapter contains a base-lacking spacer which will terminate reverse transcription in a subsequent step; hybridizing a 3?-Db-adapter to the 3?-end of target RNAs, wherein the 3?-Db-adapter has a stem-loop structure whose protruding 3?-end base-pairs 3?-end of target RNAs; ligating the both adapters with target RNAs by Rnl2 ligation to form “dumbbell-like” structure; and, amplifying and quantifying the ligation product by TaqMan RT-PCR.

Abstract: The present invention relates to systems, devices and methods for diagnosing cancer. In various embodiments, the present invention provides a method for quantifying a 5?-htRNA; a method for quantifying a 3?-htRNA; a method for obtaining a DNA library of 5?-htRNAs and a DNA library of 5?-htRNAs obtained therefrom; and a method for obtaining a DNA library of 3?-htRNAs and a DNA library of 3?-htRNAs obtained therefrom. The invention also teaches a method for determining the presence or absence of a cancer cell in a biological sample; a method of diagnosing cancer in a subject; and a method of prognosing cancer in a subject.

Abstract: The injection molding machine comprises the closing mechanism 5 having the nozzle touch member 3 to close the tip opening 1a of the nozzle 1 and the electric heater 6 to regulate the temperature of the nozzle touch member 3. The closing member 5 is constituted in such a manner as the arm 13 is pivotally supported on the jacket cover 11 mounted on the nozzle 1, the cylinder 7 is mounted on mounting member 14 provided at the tip of the arm 13, and the nozzle touch member 3 is supported at the tip of the piston rod 7a. The electric heater 6 is arranged around the nozzle touch member 3. When the arm 13 is rotated from the retreated position R to the closed position C by the actuation of the rotor 8, the nozzle touch member 3 is faced to the tip opening 1a of the nozzle 1, and by extending the cylinder 7, the nozzle touch member 3 regulated to the temperature suitable for the resin material by the electric heater 6 closes the tip opening 1a from its front surface.

Abstract: In an injection molding machine, a fixed platen 2 is provided with an air cylinder 11 through a bracket 13 and a nozzle touch plate 10 is coupled to an end of a piston rod 12 of the air cylinder 11. The piston rod 12 is extendably and contractedly driven so that the nozzle touch plate 10 is moved forward and backward between a closed position where the tip opening 9a of the nozzle 9 can be closed and a retreated position where the opening 9a can be opened. On the nozzle touch plate 10, a spherical recess portion slightly larger in radius than the tip sphere of the nozzle 9 is formed as an abutment portion 10a, and a heater 14 for adjusting the temperature is disposed in the nozzle touch plate 10. Alternatively, a passage may be formed in the nozzle touch plate 10 so that a medium of an optimum temperature can be circulated and supplied.

Abstract: A treating method for improving the strength of molded products. Molded products of a thermosetting resin containing a phenol resin as a main component are taken out of a mold of an injection molding machine, and then immediately immersed in water or exposed to blown air. With this treatment, the breaking strength, particularly the toughness of the molded products is increased. This method provides molded products having a large strength at room temperature as well as at temperatures around 120.degree. C.