Abstract: The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligo-nucleotides can be used in a variety of nucleic acid sequencing methods.

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.

Abstract: An image processing method includes obtaining a sample image, a category label of the sample image, and a label value of the category label. The method further includes calling a preset image processing model to perform segmentation processing on the sample image to obtain at least two sub-regions. The method further includes calling the preset image processing model to perform category prediction on the sample image to obtain a predicted value of the category label. The method further includes updating a network parameter of the preset image processing model according to center coordinates of the sub-regions, the label value of the category label, and the predicted value of the category label. The method further includes performing iterative training on the preset image processing model according to the updated network parameter, to obtain a target image processing model.

Abstract: This application relates to an image segmentation method and apparatus. The method includes obtaining a current frame and historical affine transformation information transmitted by a previous video frame in a video frame sequence; performing affine transformation on the current frame according to the historical affine transformation information to obtain a candidate region image corresponding to the current frame; performing feature extraction on the candidate region image to obtain a feature map corresponding to the candidate region image; performing semantic segmentation based on the feature map to obtain a segmentation result corresponding to a target in the current frame; and revising the historical affine transformation information according to the feature map to obtain updated affine transformation information, and using the updated affine transformation information as historical affine transformation information corresponding to a subsequent video frame in the video frame sequence.

Abstract: Provided herein are methods, and compositions for the real-time detection of protein synthesis. The methods are applicable to monitoring on a microfluidic device.

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.

Abstract: A semantic segmentation method and apparatus for a three-dimensional image, and a storage medium are provided. The method includes: obtaining a three-dimensional image; slicing the three-dimensional image according to three directional planes, to obtain two-dimensional slice images of an x axis, two-dimensional slice images of a y axis, and two-dimensional slice images of a z axis; invoking a first segmentation model, a second segmentation model, and a third segmentation model to respectively perform semantic segmentation on the two-dimensional slice images of the x axis, the y axis, and the z axis, to obtain distribution probability maps of a target object on the three directional planes; and obtaining a three-dimensional distribution binary image of the target object by invoking an adaptive fusion model to perform three-dimensional fusion on the three distribution probability maps respectively corresponding to an x-axis directional plane, a y-axis directional plane, and a z-axis directional plane.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: A target detection method is provided for a computing device. The method includes obtaining a current frame and a previous key frame corresponding to the current frame in a video frame sequence, determining a flow feature map and a flow field between the previous key frame and the current frame, obtaining, in response to determining the current frame is a non-key frame according to the flow feature map, a key frame feature corresponding to the previous key frame, and performing affine transformation on the key frame feature according to the flow field to obtain an image feature corresponding to the current frame, and performing target detection on the current frame according to the image feature to obtain a target detection result of the current frame.

Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of PoIµ, PoI?, PoI?, and PoI? of any species or the homologous amino acid sequence of X family polymerases of any species in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases or homologous enzymes and 3?-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.

Abstract: The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligonucleotides can be used in a variety of nucleic acid sequencing methods.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Abstract: The present invention is directed to compositions, methods and kits useful for the generation of nucleic acids from RNA templated and further nucleic acid amplification and detection. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase polymerase chain reaction (RT-PCR). Provided are compositions and methods for improved amplification of nucleic acid molecules in a two-step, addition only RT-PCR procedure. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules, and is useful for a variety of research, industrial, medical and forensic purposes.

Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

Abstract: An image segmentation method is provided for a computer device. The method includes obtaining a plurality of sample images, calling an initial model to input the plurality of sample images into the initial model and to train the initial model based on the plurality of sample images to obtain an image segmentation model and, based on the initial model, determining a number of image segmentation modules according to a number of types of pixels of the plurality of sample images. Different image segmentation modules are used for segmenting different regions of an image. The method also includes calling the image segmentation model in response to obtaining a first image to be segmented, and segmenting the first image by using the image segmentation model based on a plurality of image segmentation modules, to output a second image. Computer device and non-transitory computer-readable storage medium counterparts are also contemplated.

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Pol?, Pol?, Pol?, and Pol? of any species or the homologous amino acid sequence of X family polymerases of any species and uses thereof.

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Pol?, Pol?, Pol?, and Pol? of any species or the homologous amino acid sequence of X family polymerases of any species and their use in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of new terminal deoxynucleotidyl transferases and 3?-blocked nucleoside triphosphates in a method of template independent nucleic acid synthesis.