Patents by Inventor Sophie J. Boguslawski

Sophie J. Boguslawski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5492813
    Abstract: Muteins of enzyme acceptor polypeptide fragments of .beta.-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
    Type: Grant
    Filed: November 1, 1993
    Date of Patent: February 20, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Scott J. Eisenbeis, Sophie J. Boguslawski, Mark Krevolin, David J. Ledden
  • Patent number: 5464747
    Abstract: A mutein of an enzyme acceptor polypeptide fragment of .beta.-galactosidase which is resistant to oxidation is provided. The enzyme acceptor fragment has an amino acid other than cysteine located at position 602 of the natural sequence. An especially preferred substitution is serine. Also provided are a method for producing the novel mutein, a reagent composition comprising the novel mutein, and an immunoassay method for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
    Type: Grant
    Filed: October 29, 1993
    Date of Patent: November 7, 1995
    Assignee: Boehringer Mannheim Corporation
    Inventors: Scott J. Eisenbeis, Mark Krevolin, Christopher P. Bryant, Sophie J. Boguslawski, David J. Ledden, Scott Clark
  • Patent number: 4689294
    Abstract: The rate of hybridization between two complementary polynucleotide segments in an aqueous medium is increased by the presence of the anionic polymers polyacrylate and polymethacrylate. The acceleration effect is particularly useful in nucleic acid hybridization assays involving immobilization of sample nucleic acids and the use of labeled probes. Nonspecific binding of probe to nitrocellulose supports is substantially lower in the presence of the present polymers than in the presence of the prior art accelerator dextran sulfate. Polyacrylate is particularly advantageous since it has been found to be effective at low concentrations and is significantly less expensive than the prior art compound.
    Type: Grant
    Filed: November 19, 1984
    Date of Patent: August 25, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Sophie J. Boguslawski, Leslie H. DeRiemer Anderson