Abstract: A microfluidic pump with a movable drive membrane (diaphragm), a pumping chamber, at least two fluidic ports on one side of the diaphragm, and at least one control port on an opposing side of the diaphragm is provided. A flexible drive membrane intersects the pumping chamber such that a pressure chamber is created on the side of the drive membrane which is open to the control port, and a fluid flow chamber is created on the side of the drive membrane which is open to the fluidic ports. The drive membrane acts as a valve sealing and unsealing the fluidic ports and fluid flow through the flow chamber. The microfluidic pump has an asymmetric design which facilitates the sealing and unsealing of the fluidic ports by the pressure differential in the pressure chamber.

Abstract: A sequencing manifold for the purpose of supplying control and supply services of pre-determined temporal sequences to fluid processing assemblies is provided. The functioning of this sequencing manifold requires that translation be applied to the sequencing ports. Actuator mechanisms may supply such translation as either continuous motion or as a series of stepwise motions. Actuator mechanism can be obtained that rely on only mechanical means without the need for a source of electricity. With such actuators, it becomes feasible to conduct the operations of fluid processing assemblies in remote and primitive locations that lack a source of electricity. One skilled in the mechanical arts can provide various actuator mechanisms to meet these requirements.

Abstract: Systems and methods that enable automated processing of specimens carried on microscope slides are described herein. Aspects of the technology are directed, for example, to automated specimen processing systems configured to use microfluidic slide processing modules to robotically process tissue specimens. The slide processing modules can include reagents and a flow cell with a reaction chamber for holding the tissue specimens and reagent.

Abstract: The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract: The subject of this application is a sequencing manifold for the purpose of supplying control and supply services of pre-determined temporal sequences to fluid processing assemblies. The functioning of this sequencing manifold requires that translation be applied to the sequencing ports. Actuator mechanisms may supply such translation as either continuous motion or as a series of stepwise motions. Actuator mechanism can be obtained that rely on only mechanical means without the need for a source of electricity. With such actuators, it becomes feasible to conduct the operations of fluid processing assemblies in remote and primitive locations that lack a source of electricity. One skilled in the mechanical arts can provide various actuator mechanisms to meet these requirements. The figures included below with the description of attributes are intended to convey an understanding of the mechanical principles underpinning the operation of the sequencing manifold.

Abstract: Disclosed herein are devices and methods for storing, processing, preparing and/or analyzing samples. The inventions herein also relate to strategies and methods for automating device operations and for combining multiple devices in an integrated platform.

Abstract: The present invention concerns a microfluidic system comprising: a microchannel containing several elements of two non-miscible fluids, the microchannel comprising a droplet (30) containing magnetic particles (M), and a device for generating inside the microchannel magnetic field, said device comprising an activable magnetic element, the activable magnetic element comprising a tip (5,6), the microfluidic system being configured to transport the droplet by flow or by pressure difference.

Abstract: The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.

Abstract: The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preparing and/or analyzing samples (e.g., biospecimen samples) by using one or more of capture regions and/or automated analysis.

Abstract: The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preservation or storage of samples (e.g., biospecimen samples) by using one or more of a bridge, a membrane, and/or a desiccant.

Abstract: Provided herein are devices and methods for generating positive and negative pressures. The devices and methods are suited for the generation of pressures; in particular, the pressures generated can be useful for controlling the flow of fluids, such as in fluidic device.

Abstract: Provided herein are methods, compositions, and devices for the parallel handling of samples, such as cells or other biological samples. The methods, compositions, and devices are suited for multiple levels of analysis, including genetic and functional assays, of samples.

Abstract: The present invention concerns a microfluidic system comprising: a microchannel containing several elements of two non-miscible fluids, the microchannel comprising a droplet (30) containing magnetic particles (M), and a device for generating inside the microchannel magnetic field, said device comprising an activable magnetic element, the activable magnetic element comprising a tip (5,6), the microfluidic system being configured to transport the droplet by flow or by pressure difference.

Abstract: The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preservation or storage of samples (e.g., biospecimen samples) by using one or more of a bridge, a membrane, and/or a desiccant.