Abstract: The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media.

Abstract: The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media.

Abstract: The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media.

Abstract: Various embodiments of the invention improve the dynamic response and output voltage accuracy in buck DC-DC converters. In certain embodiments, power efficiency is improved by storing and recycling energy that is otherwise lost during successive load transient events.

Abstract: The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media.

Abstract: A digital image includes a plurality of pixels arranged in an array. In a method of analyzing the image, some of the pixels are purposefully not processed. In particular, only those pixels in a particular subgroup are processed according to a Hough or similar transform. The number of pixels in the subgroup is less than the total number of pixels in the image (e.g., as little as about 5% of the total pixels), and each pixel in the subgroup is pseudo-randomly selected. The Hough transform is inherently configured to function within the context of noisy images, for identifying features of interest in the image, as simulated by the pseudo-random selection and processing of less than the total number of pixels in the image. This significantly reduces the processor resources required to analyze the image.

Abstract: A digital image includes a plurality of pixels arranged in an array. In a method of analyzing the image, some of the pixels are purposefully not processed. In particular, only those pixels in a particular subgroup are processed according to a Hough or similar transform. The number of pixels in the subgroup is less than the total number of pixels in the image (e.g., as little as about 5% of the total pixels), and each pixel in the subgroup is pseudo-randomly selected. The Hough transform is inherently configured to function within the context of noisy images, for identifying features of interest in the image, as simulated by the pseudo-random selection and processing of less than the total number of pixels in the image. This significantly reduces the processor resources required to analyze the image.

Abstract: A digital image includes a plurality of pixels arranged in an array. In a method of analyzing the image, some of the pixels are purposefully not processed. In particular, only those pixels in a particular subgroup are processed according to a Hough or similar transform. The number of pixels in the subgroup is less than the total number of pixels in the image (e.g., as little as about 5% of the total pixels), and each pixel in the subgroup is pseudo-randomly selected. The Hough transform is inherently configured to function within the context of noisy images, for identifying features of interest in the image, as simulated by the pseudo-random selection and processing of less than the total number of pixels in the image. This significantly reduces the processor resources required to analyze the image.

Abstract: Techniques are disclosed to control output power from a switching power supply. A sample power converter controller circuit includes a control circuit coupled to be coupled to a switch. The control circuit includes a current sense circuit to be coupled to a primary winding of an energy transfer element coupled to the switch. The control circuit also includes a measurement circuit to be coupled to a secondary winding of the energy transfer element. A feedback circuit is also included and is coupled to the control circuit and coupled to receive a feedback-signal derived from an output of a power converter. The feedback circuit is coupled to output a signal to the measurement circuit. An output of the measurement circuit and an output of the current sense circuit are coupled to control a switching of the switch to regulate a combination of an output voltage and an output current of the power converter.

Abstract: A digital image includes a plurality of pixels arranged in an array. In a method of analyzing the image, some of the pixels are purposefully not processed. In particular, only those pixels in a particular subgroup are processed according to a Hough or similar transform. The number of pixels in the subgroup is less than the total number of pixels in the image (e.g., as little as about 5% of the total pixels), and each pixel in the subgroup is pseudo-randomly selected. The Hough transform is inherently configured to function within the context of noisy images, for identifying features of interest in the image, as simulated by the pseudo-random selection and processing of less than the total number of pixels in the image. This significantly reduces the processor resources required to analyze the image.

Abstract: Techniques are disclosed to control output power from a switching power supply. For example, a switching regulator according to aspects of the present invention includes a switch to be coupled to an energy transfer element of a power supply. A controller is coupled to the switch to control a switching of the switch to regulate an output voltage and an output current at an output of power supply output. A feedback circuit is coupled to the controller. The feedback circuit is coupled to receive a feedback signal from the output of the power supply. Combinations of the output voltage and the output current correspond to output regions. There is at least one regulated output region and one unregulated output region. At least one unregulated output region is a self protection auto-restart region. Within at least one unregulated output region, the switching regulator provides continuous output power at substantially the maximum output power of the switching regulator.

Abstract: Disclosed herein are multimeric molecular complexes and compounds that are multivalent, i.e., they have two or more targeting elements directed to a ligand that confers paracellular transporting properties and/or transcytotic properties to complexes and compounds to which it is bound. The complexes and compounds have properties that are different from the properties of monomers, complexes and compounds having only one targeting element directed to a paracellular and/or transcytotic ligand. The complexes and compounds of the invention undergo endocytosis, transcytosis and exocytosis; following endocytosis, the complexes or compounds may be transported into the cytosol or an organelle of a cell. In polarized cells, transcytosis can proceed in a “forward” or “reverse” direction. Reverse transcytosis is used for the non-invasive delivery of biologically active agents from the lumen of, e.g., the gastrointestinal tract or the airways of lungs, to the circulatory system.

Abstract: Disclosed herein are complexes and compounds that pass through cellular barriers to deliver compounds into, through and out of cells, and methods of producing and using such complexes and compounds. The complexes and compounds of the invention comprise a biologically active portion and a targeting element directed to a ligand that confers transcellular, transcytotic or paracellular transporting properties to an agent specifically bound to the ligand. Also disclosed are complexes and compounds that comprise two or more targeting elements directed to a ligand that confers transcellular, transcytotic or paracellular transporting properties to an agent specifically bound to the ligand. Preferred ligands include but are not limited to the stalk of pIgR, a pIgR domain, an amino acid sequence that is conserved among pIgR's from different animals, and one of several regions of pIgR defined herein.

Abstract: The present invention relates to compositions and methods for the delivery of biologically-relevant moieties to subjects in need thereof. Particles comprising a physically entrapped targeting moiety, or a moiety that binds thereto, can promote paracellular transport, active transport, endocytosis or transcytosis into, through, out of and around, cells, tissues, and organs, optionally to an intracellular location. Such particles can provide targeted delivery of therapeutic, diagnostic, or imaging molecules.

Abstract: In an infrared obstruction detection system, an apparatus is used to detect the presence of ambient light and initiate compensation to minimize the effects of the ambient light on the performance of the system. In one embodiment, the magnitude of the ambient light is measured and an offset proportional to that magnitude is fed to the obstruction detection system such that the data indicative of an obstacle are not obscured. In another embodiment, the apparatus continually monitors the effect of ambient light and adjusts the obstacle detection information during the changing states of the ambient light detected.

Abstract: In an infrared obstruction detection system, an apparatus is used to detect the presence of ambient light and initiate compensation to minimize the effects of the ambient light on the performance of the system. In one embodiment, the magnitude of the ambient light is measured and an offset proportional to that magnitude is fed to the obstruction detection system such that the data indicative of an obstacle are not obscured. In another embodiment, the apparatus continually monitors the effect of ambient light and adjusts the obstacle detection information during the changing states of the ambient light detected.

Abstract: An object detection system employs a photo-emitter and photo-detector for synchronously detecting and processing an optical signal reflected from an object in a pinch zone of a window or door opening. A photo-emitter light signal is modulated by a modulation signal having an active phase and an inactive phase. The optical detector provides an optical detector signal that is a function of the intensity of the received light. The detected light signal is synchronously detected using a switching amplifier that multiplies the reflected modulated light signal by a first gain during the active phase and by a second gain during the inactive phase. The duration of the active and inactive phases and the first and second gains are selected such that the system gain will average to zero for ambient light when integrated over a predetermined measurement period. The synchronously detected signal is subtracted from a predetermined offset voltage, and this difference is then integrated over the measurement period.

Abstract: A system and method are disclosed for enabling the selective monitoring of various regions of an aperture having a powered closure operative therein. Each of plural, individually selectable emitters is adapted to provide a narrow beam whose angle with respect to a horizontal plane is offset from the other emitters. All of the radiated beams lie in substantially the same plane in azimuth. A controller responds to certain stimuli to selectively activate one or more of the emitters. The energy thus produced is monitored by a receiver preferably disposed within the same housing. The receiver output is analyzed by the controller to identify the presence of an obstacle in that portion of the aperture illuminated by the selected emitter(s). A variety of systems may provide input to the controller for the purpose of influencing which of the plural emitters are to be activated for object detection.

Abstract: An object detection system employs a photo-emitter and photo-detector for synchronously detecting and processing an optical signal reflected from an object in a pinch zone of a window or door opening. A photo-emitter light signal is modulated by a modulation signal having an active phase and an inactive phase. The optical detector provides an optical detector signal that is a function of the intensity of the received light. The detected light signal is synchronously detected using a switching amplifier that multiplies the reflected modulated light signal by a first gain during the active phase and by a second gain during the inactive phase. The duration of the active and inactive phases and the first and second gains are selected such that the system gain will average to zero for ambient light when integrated over a predetermined measurement period. The synchronously detected signal is subtracted from a predetermined offset voltage, and this difference is then integrated over the measurement period.

Abstract: An object detection system employs a photo-emitter and photo-detector for synchronously detecting and processing an optical signal reflected from an object in a pinch zone of a window or door opening. A photo-emitter light signal is modulated by a modulation signal having an active phase and an inactive phase. The optical detector provides an optical detector signal that is a function of the intensity of the received light. The detected light signal is synchronously detected using a switching amplifier that multiplies the reflected modulated light signal by a first gain during the active phase and by a second gain during the inactive phase. The duration of the active and inactive phases and the first and second gains are selected such that the system gain will average to zero for ambient light when integrated over a predetermined measurement period. The synchronously detected signal is subtracted from a predetermined offset voltage, and this difference is then integrated over the measurement period.