Patents by Inventor Stephen L. Pentoney

Stephen L. Pentoney has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190275208
    Abstract: Disclosed are compositions comprising cellular constructs comprising mesenchymal cells and epithelial cells. Also disclosed are methods of making the cellular constructs, methods of hair restoration, and kits. The invention also discloses parallel bio-printing systems and methods for making cellular constructs, such as cellular constructs comprising mesenchymal cells and epithelial cells.
    Type: Application
    Filed: November 10, 2017
    Publication date: September 12, 2019
    Inventors: Emily BOZEK, Jonah COOL, Harry S. RAPOPORT, Christian HOLMES, Stephen L. PENTONEY, Chad VIERGEVER, Howard HWANG, Christopher SHAW
  • Patent number: 7518727
    Abstract: A system for detecting electromagnetic radiation from samples comprising: a plurality of sample volumes, each of said sample volumes confined within a portion of a capillary column; a plurality of electromagnetic radiation sources; a mirror for receiving electromagnetic radiation from the electromagnetic radiation sources and for reflecting the electromagnetic radiation to the sample volumes; a scanner attached to the mirror; a parabolic reflector for collecting sample electromagnetic radiation from the sample volumes, the sample electromagnetic radiation being generated as a result of interaction of the reflected electromagnetic radiation with the sample volumes; a plurality of filters for filtering the sample electromagnetic radiation; and a plurality of detectors for detecting sample electromagnetic radiation from the sample volumes, each of the detectors being configured to receive sample electromagnetic radiation that has passed through a corresponding one of the plurality of filters and generate a signa
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: April 14, 2009
    Assignee: Beckman Coulter, Inc.
    Inventors: Stephen L. Pentoney, Jr., David L. Yang
  • Publication number: 20080203319
    Abstract: A system for detecting electromagnetic radiation from samples comprising: a plurality of sample volumes, each of said sample volumes confined within a portion of a capillary column; a plurality of electromagnetic radiation sources; a mirror for receiving electromagnetic radiation from the electromagnetic radiation sources and for reflecting the electromagnetic radiation to the sample volumes; a scanner attached to the mirror; a parabolic reflector for collecting sample electromagnetic radiation from the sample volumes, the sample electromagnetic radiation being generated as a result of interaction of the reflected electromagnetic radiation with the sample volumes; a plurality of filters for filtering the sample electromagnetic radiation; and a plurality of detectors for detecting sample electromagnetic radiation from the sample volumes, each of the detectors being configured to receive sample electromagnetic radiation that has passed through a corresponding one of the plurality of filters and generate a signa
    Type: Application
    Filed: February 28, 2007
    Publication date: August 28, 2008
    Inventors: Stephen L. Pentoney, David L. Yang
  • Patent number: 7300800
    Abstract: An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.
    Type: Grant
    Filed: May 13, 2005
    Date of Patent: November 27, 2007
    Assignee: Beckman Coulter, Inc.
    Inventors: Michael L. Bell, Yuan Lin, Josephine M. Michael, Stephen L. Pentoney, Jr., Tsong-Tseh Tsay
  • Patent number: 7179357
    Abstract: The invention is a method of performing electrophoresis that increases sample throughput and increases the efficiency and speed of the analysis of polynucleotides by electrophoresis. The method is performed by loading and running multiple sequential samples on each capillary gel without flushing or replacing the gel between samples.
    Type: Grant
    Filed: October 25, 2002
    Date of Patent: February 20, 2007
    Assignee: Beckman Coulter, Inc.
    Inventors: Clarence Lew, Stephen L. Pentoney, Jr., David L. Yang
  • Patent number: 6962820
    Abstract: An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.
    Type: Grant
    Filed: February 12, 2004
    Date of Patent: November 8, 2005
    Assignee: Beckman Coulter, Inc.
    Inventors: Michael L. Bell, Yuan Lin, Josephine M. Michael, Stephen L. Pentoney, Jr., Tsong-Tseh Tsay
  • Patent number: 6838289
    Abstract: An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: January 4, 2005
    Assignee: Beckman Coulter, Inc.
    Inventors: Michael L. Bell, Yuan Lin, Josephine M. Michael, Stephen L. Pentoney, Jr., Tsong-Tseh Tsay
  • Publication number: 20040214176
    Abstract: Oligonucleotide probes, kits, and methods useful for detecting more than one target nucleic acid at a time are provided. The invention provides oligonucleotides that interact with target nucleic acids to form first stage cleavage structures that are cleaved by a structure-specific nuclease. In an embodiment, the cleavage products of the first stage cleavage reactions interact with secondary cleavage oligonucleotides that are specific for each target nucleic acid and that are coupled to a solid support. The cleavage of the specific secondary cleavage oligonucleotides leads to detectible changes in fluorescence and is used to detect multiple target nucleic acids in one assay. In one embodiment, the solid support is a coded bead.
    Type: Application
    Filed: April 22, 2003
    Publication date: October 28, 2004
    Inventors: James C. Osborne, John P. Wareham, Stephen L. Pentoney, Martin F. Siaw
  • Publication number: 20040166595
    Abstract: An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.
    Type: Application
    Filed: February 12, 2004
    Publication date: August 26, 2004
    Inventors: Michael L. Bell, Yuan Lin, Josephine M. Michael, Stephen L. Pentoney, Tsong-Tseh Tsay
  • Publication number: 20040079640
    Abstract: The invention is a method of performing electrophoresis that increases sample throughput and increases the efficiency and speed of the analysis of polynucleotides by electrophoresis. The method is performed by loading and running multiple sequential samples on each capillary gel without flushing or replacing the gel between samples.
    Type: Application
    Filed: October 25, 2002
    Publication date: April 29, 2004
    Inventors: Clarence Lew, Stephen L. Pentoney, David L. Yang
  • Publication number: 20030207461
    Abstract: An analyte detection system utilizing a combination of fluorescent labels for labeling particles and an analyte specific fluorescent analyte detection dye. The particles contain a combination of fluorescent labels for coding the particles and an analyte specific fluorescent dye. The particles can be used to identify and quantify analytes in an analytical sample by reaction of the analytical sample with the particles. An analytical device can identify the particles according to the combination of fluorescent labels. The device can then correlate the identified particle with the analyte specific fluorescent analyte detection dye. Multiple subpopulations of particles can be used to identify and quantify multi-analytes in a single analytical sample. Near infrared (NIR) fluorescent labels useful in the detection system are also provided.
    Type: Application
    Filed: November 14, 2001
    Publication date: November 6, 2003
    Inventors: Michael L. Bell, Yuan Lin, Josephine M. Michael, Stephen L. Pentoney, Tsong-Tseh Tsay
  • Patent number: 5900132
    Abstract: A holder (10) is provided for securing a capillary (30) having a window section (28) including first and second edges (46) (48) to a mount (80). The holder (10) comprises a retaining surface (14) for securing the capillary (30) to the holder (10) and a mounting surface (18) for detachably securing the holder (10) to the mount (80). The retaining surface (14) is attached to the capillary (30) proximate to the opposed first and second edges (46), (48), with a portion of the window section (28) exposed. Since the capillary (30) is secured proximate the opposed first and second edges (46), (48), the fragile capillary window section (28) is protected during manufacturing or installation into an electrophoretic system (32). The retaining surface (14) preferably is sized and shaped to retain a plurality of capillaries (30) to allow for the substantially simultaneous testing of multiple capillaries (30).
    Type: Grant
    Filed: June 28, 1996
    Date of Patent: May 4, 1999
    Assignee: Beckman Coulter, Inc.
    Inventors: Charles A. Keenan, Stephen L. Pentoney, Jr., Brian D. Peterson, Jack D. McNeal
  • Patent number: 5675155
    Abstract: A detection system for sequentially and repetitively scanning a plurality of sample volumes and detecting electromagnetic radiation emitting from each of the sample volumes includes a plurality of coplanar side-by-side capillaries each containing a sample volume, an electromagnetic radiation source, a mirror aligned to receive and reflect electromagnetic radiation, a scanner for moving the mirror, and a detector aligned to receive electromagnetic radiation collected from the sample volumes. In operation, the scanner adjusts the mirror position so that the mirror is positioned to receive the electromagnetic radiation from the electromagnetic radiation source and reflect the electromagnetic radiation sequentially and repetitively to the sample volumes. Emitted electromagnetic radiation from the sample volume is collected and directed to a detector where a signal is generated in response to the interaction of the electromagnetic radiation with the sample.
    Type: Grant
    Filed: April 26, 1995
    Date of Patent: October 7, 1997
    Assignee: Beckman Instruments, Inc.
    Inventors: Stephen L. Pentoney, Jr., David J. Rakestraw
  • Patent number: 5635050
    Abstract: A gel pump operated by a stepper motor may be used to deliver fresh gel to one or more capillaries through one or more manifolds and valves. By controlling the valves, fresh gel delivered by the pump will replace the old gel in the capillaries for an automated gel replacement system. In a different setting of the valves, the manifolds may be purged of the old gel prior to delivery of fresh gel to the capillaries. The gel delivery system may also be combined with an electrophoresis system so that sealing connection capable of withstanding high pressure adequate for gel injection need not be frequently broken when the gel is to be replaced. Manifold/reflector assembly is advantageously used in the system that facilitates electrical circuit for electrophoresis and for gel replacement.
    Type: Grant
    Filed: August 23, 1995
    Date of Patent: June 3, 1997
    Assignee: Beckman Instruments, Inc.
    Inventors: Stephen L. Pentoney, Jr., Brian D. Peterson, James C. Osborne, Charles A. Keenan
  • Patent number: 5630925
    Abstract: An electrophoretic system employing a capillary with at least one end having an electrically conductive coating or layer directly thereon. In order to introduce a plug of sample from a small amount of the sample in a vial, the conductive tip is submersed into the small amount of sample and an electric field is applied at the end by applying the high voltage to the tip in order to electrokinetically inject a plug of the sample. The conductive tip may also be used as a terminal for completing the circuit for applying the high voltage across the capillary column for electrophoresis. The separated components may be collected on a surface or small amount of buffer to reduce sample dilution or mixing of one sample component with a different sample component from the electrophoretic process.
    Type: Grant
    Filed: July 13, 1995
    Date of Patent: May 20, 1997
    Assignee: Beckman Instruments, Inc.
    Inventors: Stephen L. Pentoney, Jr., Kenneth D. Konrad
  • Patent number: 5614726
    Abstract: A system and method are disclosed for optically aligning a capillary tube and an excitation laser beam for fluorescence detection applications by utilizing the Raman scatter signals of the capillary tube's contents. For example, Raman scatter by an electrophoretic separation matrix may be used for alignment in a capillary electrophoresis system. Fluorescent material may be present and may also be used for alignment purposes, but is not necessary. The invention employs a parabolic reflector, having apertures through which the capillary tube and the laser beam are guided so that they intersect, preferably at right angles and at the focal point of the reflector. The Raman scatter signals of the material within the capillary tube are collected via a series of filters and this information is used to reposition, if necessary, a focusing lens that directs the excitation beam into the reflector and the capillary tube, so that the Raman scatter signals are maximized.
    Type: Grant
    Filed: March 23, 1995
    Date of Patent: March 25, 1997
    Assignee: Beckman Instruments, Inc.
    Inventors: Wilbur I. Kaye, Stephen L. Pentoney, Jr.
  • Patent number: 5545901
    Abstract: An apparatus and method are set for determining the centers of capillaries in a multicapillary electrophoresis array for subsequent rapid and efficient laser induced fluorescence interrogation. A galvometric scanner reflects the laser beam to scan the array during an alignment pre-scan. The beam interacts with the array to produce an intensity pattern indicative of the capillary centers. The scanner positions corresponding to the capillary centers determined during the pre-scan is recalled to direct the beam during the interrogation scan to the column centers.
    Type: Grant
    Filed: August 23, 1995
    Date of Patent: August 13, 1996
    Assignee: Beckman Instruments, Inc.
    Inventors: Stephen L. Pentoney, Jr., Clarence Y. Lew, David Rakestraw
  • Patent number: 5534707
    Abstract: An apparatus and method of aligning a capillary with respect to a radiation source. More particularly, the capillary is aligned with a laser beam for laser induced fluorescence detection. The scatter or transmitted light pattern of the laser beam with respect to the capillary tube is utilized to determine optimum alignment. photosensors may be implemented to detect the light pattern which represents optimum alignment. For dynamic alignment during electrophoresis, the photosensors provide feedback to a controller which controls a positioning mechanism for alignment of the capillary.
    Type: Grant
    Filed: March 29, 1994
    Date of Patent: July 9, 1996
    Assignee: Beckman Instruments, Inc.
    Inventor: Stephen L. Pentoney
  • Patent number: 5484571
    Abstract: A simple device for collecting a greater amount of fluorescent emission from a minute sample in a capillary column. An axially symmetrical paraboloid reflector is implemented to collect fluorescent emission from the capillary column. The reflector also serves as a simple bracket for positioning and aligning the capillary column. Fluorescent emission is collimated by the paraboloid reflector which allows more effective use of band pass filters in blocking scattered radiation from detection. The scattered and transmitted radiation can be used to facilitate alignment of the capillary with respect to the detection optics. The paraboloid reflector also facilitates the implementation of simultaneous multiple-channel detection.
    Type: Grant
    Filed: June 23, 1994
    Date of Patent: January 16, 1996
    Assignee: Beckman Instruments, Inc.
    Inventors: Stephen L. Pentoney, Jr., Wilbur Kaye
  • Patent number: 5298134
    Abstract: A cross or T-shaped device is disclosed for use in capillary electrophoresis or capillary chromatography. The device includes a first capillary tube and a second capillary tube connected to the first tube at a point between the two ends of the first tube so that the contents flowing in the second tube will mix with a fluid flowing in the first tube. The two tubes enclose spaces with cross-sectional dimensions less than about 200 microns. The two tubes are connected so that there is substantially no dead space at the connection. The device is made by boring a hole at a selected location in the first tube, introducing an elongated guide member into the hole, threading the member into the second tube until the second tube contacts the first tube. The second tube is then permanently connected to the first tube and the guide member is then removed to form a T-shaped device.
    Type: Grant
    Filed: May 20, 1993
    Date of Patent: March 29, 1994
    Assignee: Board of Trustees of the Leland Stanford Junior University
    Inventors: Richard N. Zare, Xiaohua Huang, Stephen L. Pentoney, Jr.