Abstract: The present invention relates to a new method of gene integration in cells comprising the steps of: preparation of a suspension of poly nucleotide fragments comprising the desired gene in a hypotonic solution; administration of said suspension to cells or tissues; and maintenance of the cells or tissues in vitro or in its normal physiological condition. The invention also relates to a method of generating transgenic animal.

Abstract: A pharmaceutically acceptable peroxovanadium(v) amine product, DmpzH[VO(02)2](Dmpz)], and its oral as well as injectable use for the treatment of Diabetes mellitus, wherein the said therapeutically stable compound is obtained by reacting V205 or vanadate with hydrogen peroxide and the amine, dimethylpyrazole (Dmpz), at pH 5.5 and temperature 0-4° C. DmpzH[VO(02)2](Dmpz)] is thus poised as a versatile insulin mimic adapted for targeting insulin signaling, stimulating adipogenesis, abrogating insulin stimulated down stream signals thereby lowering the incidence of insulin resistance and type-2 diabetes.

Abstract: The present invention relates to a method of in vitro isolation of buffalo ?-caesin promoter (buCSN2) along with the regions exon1, intron1 and exon2 from the genomic DNA in vitro (Bubalus bubalis) and its functional activity in using mammary cell line. The novel buffalo ?-caesin promoter along with exon1, intron1 and exon2 is isolated and cloned upstream of the Enhanced Green flourescence protein (EGFP) gene and sequenced. The transfection of the DNA construct resulted into production of EGFP protein in mammary cell lines, confirming bioactivity of this newly isolated buffalo promoter sequence. More specifically, the present invention relates to isolation, cloning, sequencing and functional analysis of the buffalo ?-casein promoter in vitro using mammary cell line.

Abstract: The present invention relates to a method of in vitro isolation of buffalo ?-caesin promoter (buCSN2) along with the regions of exon1, intron1 and exon2 from the genomic DNA in vitro (Bubalus bubalis) and its functional activity in using mammary cell line. The novel buffalo ?-caesin promoter along with exon1, intron1 and exon2 is isolated and cloned upstream of the Enhanced Green flourescence protein (EGFP) gene and sequenced. The transfection of the DNA construct resulted into production of EGFP protein in mammary cell lines, confirming bioactivity of this newly isolated buffalo promoter sequence. More specifically, the present invention relates to isolation, cloning, sequencing and functional analysis of the buffalo ?-casein promoter in vitro using mammary cell line.

Abstract: The present invention provides a novel method of generating knock down models by electroporation of shRNA construct into the testis. The present invention provides an ethically superior, non-surgical, user friendly rapid method for the generation of permanent lines of shRNA knock down non human vertebrates. This invention is ethically superior as it does not involve any loss of animal life and drastically minimizes the production time and use of animals. Current techniques for making knockout models are cumbersome, require trained personnel, costly infrastructure and require hundreds of eggs collected after killing several females. In contrast, this method neither involves any costly infrastructure nor requires trained personnel. The invention also relates to the quick incorporation of shRNA gene construct into the germline of a species so that shRNA is inheritable.