Abstract: To provide a method of easily immobilizing a compound with a wide variety of structures and functional groups onto a substrate without requiring modification and while maintaining the structures as is, a method of detecting this immobilized compound, and a compound screening method, and a screening substrate, a compound immobilizing agent, and an immobilizing kit used therein. The method of immobilizing a compound onto a substrate includes immobilizing the compound as a protein-compound complex onto the substrate via a protein for immobilizing the compound onto the substrate. Further, the compound screening method includes detecting a compound having affinity for a target compound immobilized onto the substrate by bringing a candidate compound into contact with this target compound. A screening substrate on which the immobilizing protein forms a complex with a compound is also provided.

Abstract: A method for readily and accurately differentiating an esophageal cancer patient's tumor between basaloid squamous cell carcinoma of the esophagus or another esophageal cancer is developed and provided. A method is provided for assisting differentiating contraction of basaloid squamous cell carcinoma of the esophagus, including measuring the expression levels of markers for basaloid squamous cell carcinoma of the esophagus in samples collected from a test subject and a healthy subject or a group of healthy subjects and determining whether the test subject is highly likely to be affected with basaloid squamous cell carcinoma of the esophagus based on the obtained measurement values.