Abstract: According to the present invention, purified, isolated and cloned nucleic acid polynucleotide encoding hypoxia-regulating genes and the proteins thereof and antibodies directed against the proteins which have sequences as set forth in SEQ ID No:1, SEQ ID No:2, SEQ ID No:3, SEQ ID No:4, SEQ ID No:5 and SEQ ID No:6 are provided. The present invention further provides transgenic animals and cell lines as well as knock-out organisms of these sequences. The present invention further provides methods of regulating angiogenesis or apoptosis or regulating response to hypoxic conditions in a patient in need of such treatment.

Abstract: The present invention relates in general to the field of cancer diagnostics and treatment. In particular, the present invention provides methods for identifying diagnostic and therapeutic molecules that bind to a carbohydrate antigen on the surface of a cancer cell and induce cell death, to pharmaceutical compositions comprising the molecules and uses thereof.

Abstract: According to the present invention, purified, isolated and cloned nucleic acid polynucleotide encoding hypoxia-regulating genes and the proteins thereof and antibodies directed against the proteins which have sequences as set forth in SEQ ID No:1, SEQ ID No:2, SEQ ID No:3, SEQ ID No:4, SEQ ID No:5 and SEQ ID No:6 are provided. The present invention further provides transgenic animals and cell lines as well as knock-out organisms of these sequences. The present invention further provides methods of regulating angiogenesis or apoptosis or regulating response to hypoxic conditions in a patient in need of such treatment.

Abstract: An expression system useful for the detection and isolation of a polypeptide capable of regulating a transduction pathway is provided. When expressed in a cell, the expression system provides a level of expression of a reporter molecule in cell which is indicative of regulation of the transduction pathway by a specific polypeptide of a plurality of polypeptides expressed by the cell.

Abstract: A method of detecting a polypeptide capable of regulating a transduction pathway and an expression library for use with such method are provided. The method is effected by introducing into cells endogenously expressing a trans acting regulator of the transduction pathway the expression library which includes a plurality of expression constructs each encoding a polypeptide and a reporter molecule expressible in the presence of a trans acting regulator of the transduction pathway. A level of expression within a predetermined range of the reporter is indicative of regulation of the transduction pathway by the polypeptide.

Abstract: A method of uncovering a putative functional analog of a peptide regulator of a biological pathway is disclosed. The method comprises: (a) generating a library including molecules representing: (i) constituents of the biological pathway; and/or (ii) portions of said constituents of the biological pathway; (b) contacting said molecules of said library with the peptide regulator to thereby obtain a complex composed of a molecule of said molecules of said library and the peptide regulator; (c) incubating said molecule and the peptide regulator of said complex in the presence of each of a plurality of distinct substances; and (d) identifying a substance of said plurality of distinct substances capable of competing with the peptide regulator for binding of said molecule to thereby uncover the putative functional analog of the peptide regulator of the biological pathway.

Abstract: A method for identifying genes regulated at the RNA level by cue-induced gene expression. The invention relates to the rapid isolation of differentially expressed or developmentally regulated gene sequences through analysis of mRNAs obtained from specific cellular compartments and comparing the changes in the relative abundance of the mRNA in these compartments as a result of applying a cue to the tested biological sample. The cellular compartments include polysomal and nonpolysomal fractions, nuclear fractions, cytoplasmic fractions, and spliceosomal fractions. Genes that are differentially expressed due to regulation on any one or more of a number of levels, may be characterized. Regulation levels include translational regulation, transcriptional regulation, mRNA stability regulation, and mRNA transport regulation.

Abstract: A method for identifying translationally regulated genes includes selectively stimulating translation of an unknown target mRNA with a stress inducing element wherein the target mRNA is part of a larger sample of mRNA. The mRNA sample is divided into pools of translated and untranslated mRNA which are differentially analyzed to identify genes that are translationally regulated by the stress inducing element. A method for identifying gene sequences coding for internal ribosome entry sites includes inhibiting 5' cap-dependant mRNA translation in a cell, collecting a pool of mRNA from the cells, and differentially analyzing the pool of mRNA to identify genes with sequences coding for internal ribosome entry sites.