Patents by Inventor Tadaaki Ohgi

Tadaaki Ohgi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11027023
    Abstract: The present invention provides a natural type miRNA, which is a single-stranded nucleic acid containing X region and Y region, wherein the 3?-terminus of said X region and the 5?-terminus of said Y region are linked via a linker region of a non-nucleotide structure, the X region contains (a) a guide strand sequence or (b) a passenger strand sequence of a mature miRNA, when the X region contains (a), the Y region contains a passenger strand sequence of the mature miRNA, when the X region contains (b), the Y region contains a guide strand sequence of the mature miRNA, and the guide strand sequence and the passenger strand sequence form a double-stranded structure.
    Type: Grant
    Filed: December 25, 2015
    Date of Patent: June 8, 2021
    Assignee: BONAC CORPORATION
    Inventors: Eriko Aoki, Yasuhiko Yoshida, Shiori Kato, Tadaaki Ohgi
  • Patent number: 10934542
    Abstract: The invention provides an artificial match-type miRNA utilizing miRNA. In particular, the invention provides a single strand nucleic acid containing an X region and a Y region, wherein the 3?-terminal of the X region and the 5?-terminal of the Y region are linked via a linker region of a non-nucleotide structure, the X region contains a guide strand sequence of a mature miRNA, and the Y region contains a sequence completely complementary to the X region is an artificial match-type miRNA. The artificial match-type miRNA can suppress expression of the target gene.
    Type: Grant
    Filed: December 27, 2014
    Date of Patent: March 2, 2021
    Assignees: BONAC CORPORATION, TOKYO MEDICAL UNIVERSITY
    Inventors: Masahiko Kuroda, Shinichiro Ohno, Eriko Aoki, Yasuhiko Yoshida, Shiori Kato, Tadaaki Ohgi
  • Publication number: 20200038427
    Abstract: The present invention aims to provide a nucleic acid molecule having improved stability in serum and capable of exhibiting a gene expression regulation function after transfer into the cell. A cyclic nucleic acid molecule represented by the following formula (A): wherein each symbol is as described in the DESCRIPTION can be synthesized easily at a low cost, and can inhibit the translation of a protein encoded by the gene. Since the cyclic nucleic acid molecule of the present invention can inhibit the expression of a target gene as described above, for example, it is useful as a pharmaceutical product, a diagnostic agent, an agricultural chemical, and a tool for conducting research on agriculture, medical science, life science, and the like.
    Type: Application
    Filed: March 30, 2018
    Publication date: February 6, 2020
    Applicant: BONAC CORPORATION
    Inventors: Tadaaki OHGI, Chisato EMURA, Junichi YASUOKA, Yutaka EGUCHI
  • Publication number: 20190382758
    Abstract: The invention provides an artificial sgRNA and a CRISPR/Cas9 system by combining the artificial sgRNA and Cas9. Activity of the sgRNA can be retained even when a nucleotide linker region for forming a single strand by linking the 3?-terminal of crRNA and the 5?-terminal of tracrRNA in sgRNA is substituted with an amino acid derivative linker, when the linker region existing between stem-loop 1 and stem-loop 2 of tracrRNA and/or the loop portion of stem-loop 2 are/is substituted with an amino acid derivative linker, or when an amino acid derivative linker is added/inserted into the vicinity of the 5?-terminal and/or the 3?-terminal of sgRNA. Stability in vivo can be improved by introducing one or more amino acid derivative linkers into the sgRNA.
    Type: Application
    Filed: January 30, 2017
    Publication date: December 19, 2019
    Applicant: BONAC CORPORATION
    Inventors: Eriko AOKI, Tadaaki OHGI, Takashi KINOSHITA
  • Patent number: 10377788
    Abstract: The invention provides a production method of a glycoside compound or a salt thereof, which includes subjecting a thioether compound and an alcohol compound to a coupling reaction in the presence of a halogenating agent, a desiccant and a Lewis acid, and then distillation in the presence of at least one kind of additive selected from a sulfur-containing antioxidant and a maleimide group-containing compound to give a thioether compound and a step of subjecting a glycoside compound and a thioether compound to a coupling reaction in the presence of a halogenating agent, a desiccant and a Lewis acid to give a glycoside compound. By this method, a phosphoramidite preferable for the production (synthesis) of a nucleic acid can be produced more efficiently at a high purity.
    Type: Grant
    Filed: April 1, 2016
    Date of Patent: August 13, 2019
    Assignee: BONAC CORPORATION
    Inventors: Eriko Aoki, Takashi Kinoshita, Akihiro Itoh, Tadaaki Ohgi
  • Patent number: 10238752
    Abstract: The invention provides a single-stranded nucleic acid capable of inhibiting expression of a target gene having a delivery function. The nucleic acid contains, from the 5?-side to the 3?-side, a 5?-side region (Xc), a linker region (Lx), an inner region (Z), a linker region (Ly) and a 3?-side region (Yc) in this order, wherein the inner region (Z) is constituted by linkage of the inner 5?-side region (X) and the inner 3?-side region (Y), the 5?-side region (Xc) is complementary to the inner 5?-side region (X), the 3?-side region (Yc) is complementary to the inner 3?-side region (Y), at least one of the inner region (Z), the 5?-side region (Xc) and the 3?-side region (Yc) comprises an expression inhibitory sequence that inhibits expression of a target gene, and at least one of the 5?-terminus, the 3?-terminus, the linker region (Lx) and the linker region (Ly) is bound to a bio-related substance.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: March 26, 2019
    Assignee: Bonac Corporation
    Inventors: Tadaaki Ohgi, Eriko Aoki, Chisato Emura, Tomohiro Hamasaki
  • Patent number: 10233446
    Abstract: The invention provides a microRNA inhibitor that has two or more sequences complementary to the sequence of microRNA to be the target of inhibition, which two or more complementary sequences are linked via one or more linker residues.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: March 19, 2019
    Assignee: Bonac Corporation
    Inventors: Tadaaki Ohgi, Hisao Shirohzu, Hiroshi Suzuki, Tomohiro Hamasaki, Takayuki Mizutani
  • Publication number: 20180326091
    Abstract: The present invention provides a natural type miRNA, which is a single-stranded nucleic acid containing X region and Y region, wherein the 3?-terminus of said X region and the 5?-terminus of said Y region are linked via a linker region of a non-nucleotide structure, the X region contains (a) a guide strand sequence or (b) a passenger strand sequence of a mature miRNA, when the X region contains (a), the Y region contains a passenger strand sequence of the mature miRNA, when the X region contains (b), the Y region contains a guide strand sequence of the mature miRNA, and the guide strand sequence and the passenger strand sequence form a double-stranded structure.
    Type: Application
    Filed: December 25, 2015
    Publication date: November 15, 2018
    Applicant: BONAC CORPORATION
    Inventors: Eriko AOKI, Yasuhiko YOSHIDA, Shiori KATO, Tadaaki OHGI
  • Publication number: 20180119151
    Abstract: The present invention provides a single-stranded nucleic acid molecule having a delivery function and capable of inhibiting expression of a target gene. The single-stranded nucleic acid molecule of the present invention is a single-stranded nucleic acid molecule composed of a region (Xc), a linker region (Lx) and a region (X), wherein said region (Xc) is complementary to said region (X), at least one of said region (X) and said region (Xc) contains an expression inhibitory sequence that inhibits expression of the target gene, and a bio-related substance having a delivery function is bonded to at least one selected from the group consisting of the 5?-terminus, the 3?-terminus, and said linker region (Lx).
    Type: Application
    Filed: March 26, 2016
    Publication date: May 3, 2018
    Applicant: BONAC CORPORATION
    Inventors: Eriko AOKI, Shiori KATO, Tadaaki OHGI
  • Publication number: 20180079768
    Abstract: The invention provides a production method of a glycoside compound or a salt thereof, which includes subjecting a thioether compound and an alcohol compound to a coupling reaction in the presence of a halogenating agent, a desiccant and a Lewis acid, and then distillation in the presence of at least one kind of additive selected from a sulfur-containing antioxidant and a maleimide group-containing compound to give a thioether compound and a step of subjecting a glycoside compound and a thioether compound to a coupling reaction in the presence of a halogenating agent, a desiccant and a Lewis acid to give a glycoside compound. By this method, a phosphoramidite preferable for the production (synthesis) of a nucleic acid can be produced more efficiently at a high purity.
    Type: Application
    Filed: April 1, 2016
    Publication date: March 22, 2018
    Applicant: BONAC CORPORATION
    Inventors: Eriko AOKI, Takashi KINOSHITA, Akihiro ITOH, Tadaaki OHGI
  • Publication number: 20170306325
    Abstract: The invention provides a single-stranded nucleic acid capable of inhibiting expression of a target gene having a delivery function. The nucleic acid contains, from the 5?-side to the 3?-side, a 5?-side region (Xc), a linker region (Lx), an inner region (Z), a linker region (Ly) and a 3?-side region (Yc) in this order, wherein the inner region (Z) is constituted by linkage of the inner 5?-side region (X) and the inner 3?-side region (Y), the 5?-side region (Xc) is complementary to the inner 5?-side region (X), the 3?-side region (Yc) is complementary to the inner 3?-side region (Y), at least one of the inner region (Z), the 5?-side region (Xc) and the 3?-side region (Yc) comprises an expression inhibitory sequence that inhibits expression of a target gene, and at least one of the 5?-terminus, the 3?-terminus, the linker region (Lx) and the linker region (Ly) is bound to a bio-related substance.
    Type: Application
    Filed: April 25, 2017
    Publication date: October 26, 2017
    Applicant: Bonac Corporation
    Inventors: Tadaaki OHGI, Eriko AOKI, Chisato EMURA, Tomohiro HAMASAKI
  • Patent number: 9663784
    Abstract: The invention provides a single-stranded nucleic acid capable of inhibiting expression of a target gene having a delivery function. The nucleic acid contains, from the 5?-side to the 3?-side, a 5?-side region (Xc), a linker region (Lx), an inner region (Z), a linker region (Ly) and a 3?-side region (Yc) in this order, wherein the inner region (Z) is constituted by linkage of the inner 5?-side region (X) and the inner 3?-side region (Y), the 5?-side region (Xc) is complementary to the inner 5?-side region (X), the 3?-side region (Yc) is complementary to the inner 3?-side region (Y), at least one of the inner region (Z), the 5?-side region (Xc) and the 3?-side region (Yc) comprises an expression inhibitory sequence that inhibits expression of a target gene, and at least one of the 5?-terminus, the 3?-terminus, the linker region (Lx) and the linker region (Ly) is bound to a bio-related substance.
    Type: Grant
    Filed: May 25, 2013
    Date of Patent: May 30, 2017
    Assignee: Bonac Corporation
    Inventors: Tadaaki Ohgi, Eriko Aoki, Chisato Emura, Tomohiro Hamasaki
  • Publication number: 20160376589
    Abstract: The invention provides a microRNA inhibitor that has two or more sequences complementary to the sequence of microRNA to be the target of inhibition, which two or more complementary sequences are linked via one or more linker residues.
    Type: Application
    Filed: June 30, 2016
    Publication date: December 29, 2016
    Applicant: BONAC CORPORATION
    Inventors: Tadaaki OHGI, Hisao SHIROHZU, Hiroshi SUZUKI, Tomohiro HAMASAKI, Takayuki MIZUTANI
  • Patent number: 9528111
    Abstract: The invention provides a single-stranded nucleic acid molecule containing an expression inhibitory sequence that inhibits expression of a target gene, region (X), linker region (Lx), and region (Xc), wherein the linker region (Lx) is linked between the region (Xc) and the region (Xc), the region (Xc) is complementary to the region (X), at least one of the region (X) and the region (Xc) contains the expression inhibitory sequence, and the linker region (Lx) contains an atomic group derived from an amino acid. The single-stranded nucleic acid molecule can inhibit expression of the target gene.
    Type: Grant
    Filed: December 29, 2012
    Date of Patent: December 27, 2016
    Assignee: Bonac Corporation
    Inventors: Tadaaki Ohgi, Hiroshi Suzuki, Tomohiro Hamasaki, Eriko Aoki
  • Publication number: 20160319282
    Abstract: The invention provides an artificial match-type miRNA utilizing miRNA. A In particular, the invention provides a single strand nucleic acid containing an X region and a Y region, wherein the 3?-terminal of the X region and the 5?-terminal of the Y region are linked via a linker region of a non-nucleotide structure, the X region contains a guide strand sequence of a mature miRNA, and the Y region contains a sequence completely complementary to the X region is an artificial match-type miRNA. The artificial match-type miRNA can suppress expression of the target gene.
    Type: Application
    Filed: December 27, 2014
    Publication date: November 3, 2016
    Applicants: BONAC CORPORATION, TOKYO MEDICAL UNIVERSITY
    Inventors: Masahiko KURODA, Shinichiro OHNO, Eriko AOKI, Yasuhiko YOSHIDA, Shiori KATO, Tadaaki OHGI
  • Patent number: 9404108
    Abstract: The invention provides a microRNA inhibitor that has two or more sequences complementary to the sequence of microRNA to be the target of inhibition, which two or more complementary sequences are linked via one or more linker residues.
    Type: Grant
    Filed: March 4, 2013
    Date of Patent: August 2, 2016
    Assignee: Bonac Corporation
    Inventors: Tadaaki Ohgi, Hisao Shirohzu, Hiroshi Suzuki, Tomohiro Hamasaki, Takayuki Mizutani
  • Publication number: 20160108400
    Abstract: Provided is a novel nucleic acid molecule that is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes, in sequence from the 5? side to the 3? side: a 5? side region (Xc); an inner region (Z); and a 3? side region (Yc). The inner region (Z) is composed of an inner 5? side region (X) and an inner 3? side region (Y) that are linked to each other. The 5? side region (Xc) is complementary to the inner 5? side region (X). The 3? side region (Yc) is complementary to the inner 3? side region (Y). At least one of the inner region (Z), the 5? side region (Xc), and the 3? side region (Yc) includes the expression inhibitory sequence.
    Type: Application
    Filed: October 29, 2015
    Publication date: April 21, 2016
    Applicant: BONAC CORPORATION
    Inventors: Tadaaki Ohgi, Hirotake Hayashi, Hisao Shirohzu, Tomohiro Hamasaki, Akihiro Itoh, Hiroshi Suzuki
  • Patent number: 9315799
    Abstract: The present invention provides a process for preparing an isotope-containing phosphate compound easily. The process for preparing an isotope-containing phosphate compound according to the present invention includes the step of: oxidizing a trivalent phosphorus compound with an oxidizing agent containing an isotope to synthesize a pentavalent phosphate compound to which the isotope has been introduced. The present invention preferably is applied to the synthesis of nucleic acids such as DNA and RNA, for example. The isotope preferably is a stable isotope. The oxidizing agent preferably is H218O, 3H-1,2-benzodithiol 3-one 1,1-dioxide having 34S, or a diisopropylethylamine-borane complex having 10B, for example.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: April 19, 2016
    Assignee: BONAC Corporation
    Inventors: Tomohiro Hamasaki, Tadaaki Ohgi
  • Patent number: 9206422
    Abstract: Provided is a novel nucleic acid molecule that can be produced easily and efficiently and can inhibit the expression of a gene. The nucleic acid molecule is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes: a region (X); a linker region (Lx); and a region (Xc). The linker region (Lx) is linked between the regions (Xc) and (Xc). The region (Xc) is complementary to the region (X). At least one of the regions (X) and (Xc) includes the expression inhibitory sequence. The linker region (Lx) has a non-nucleotide structure including at least one of a pyrrolidine skeleton and a piperidine skeleton. According to this single-stranded nucleic acid molecule, it is possible to inhibit the expression of the target gene.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: December 8, 2015
    Assignee: BONAC Corporation
    Inventors: Tadaaki Ohgi, Hirotake Hayashi, Hisao Shirohzu, Tomohiro Hamasaki, Akihiro Itoh, Hiroshi Suzuki
  • Patent number: 9200278
    Abstract: Provided is a novel nucleic acid molecule that can be produced easily and efficiently and can inhibit the expression of a gene. The nucleic acid molecule is a single-stranded nucleic acid molecule including an expression inhibitory sequence that inhibits expression of a target gene. The single-stranded nucleic acid molecule includes: a region (X); a linker region (Lx); and a region (Xc). The linker region (Lx) is linked between the regions (Xc) and (Xc). The region (Xc) is complementary to the region (X). At least one of the regions (X) and (Xc) includes the expression inhibitory sequence. The linker region (Lx) has a non-nucleotide structure including at least one of a pyrrolidine skeleton and a piperidine skeleton. According to this single-stranded nucleic acid molecule, it is possible to inhibit the expression of the target gene.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: December 1, 2015
    Assignee: BONAC Corporation
    Inventors: Tadaaki Ohgi, Hirotake Hayashi, Hisao Shirohzu, Tomohiro Hamasaki, Akihiro Itoh, Hiroshi Suzuki