Abstract: The exosome analysis method of the present invention comprises (a) bringing an exosome-containing sample into contact with a substrate that is modified with a compound having a hydrophobic chain and a hydrophilic chain to bind the exosome to the compound; (b) bringing the exosome into contact with a first molecule that specifically binds to a biomolecule existing on the surface of the exosome to form a first molecule-exosome complex on the substrate; and (c) detecting the first molecule-exosome complex on the substrate.

Abstract: Electrophoresis device including: a first flow passage extending in a first direction and through which a sample and a buffer solution flow; a sample collecting part provided at an end portion of the first flow passage and configured to collect the sample; electrodes disposed at both sides of the first flow passage in a second direction perpendicular to the first direction and configured to apply a voltage to the first flow passage in the second direction; second flow passages communicating with both sides of the first flow passage in the second direction, configured to accommodate the electrodes, and through which a second buffer solution flows; and partition walls fixed to communicating portions between the first and second flow passages with a predetermined bonding strength and configured to block movement of substances between the first and second flow passages. The partition walls are formed of a gel material having ion permeability.

Abstract: The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.

Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.

Abstract: Electrophoresis device including: a first flow passage extending in a first direction and through which a sample and a buffer solution flow; a sample collecting part provided at an end portion of the first flow passage and configured to collect the sample; electrodes disposed at both sides of the first flow passage in a second direction perpendicular to the first direction and configured to apply a voltage to the first flow passage in the second direction; second flow passages communicating with both sides of the first flow passage in the second direction, configured to accommodate the electrodes, and through which a second buffer solution flows; and partition walls fixed to communicating portions between the first and second flow passages with a predetermined bonding strength and configured to block movement of substances between the first and second flow passages. The partition walls are formed of a gel material having ion permeability.

Abstract: The present invention provides a fluidic device, an exosome analysis method, a biomolecule analysis method, and a biomolecule detection method, which can analyze even the content of an exosome in a series of flows by introducing a sample into the device. A fluidic device of the present invention is a fluidic device which detects a biomolecule contained in an exosome in a sample, and includes: an exosome purification unit which has a layer modified with a compound having a hydrophobic chain and a hydrophilic chain; a biomolecule purification unit; a biomolecule detection unit; a first flow path which connects the exosome purification unit to the biomolecule purification unit; and a second flow path which connects the biomolecule purification unit to the biomolecule detection unit.

Abstract: The exosome analysis method of the present invention comprises (a) bringing an exosome-containing sample into contact with a substrate that is modified with a compound having a hydrophobic chain and a hydrophilic chain to bind the exosome to the compound; (b) bringing the exosome into contact with a first molecule that specifically binds to a biomolecule existing on the surface of the exosome to form a first molecule-exosome complex on the substrate; and (c) detecting the first molecule-exosome complex on the substrate.

Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.